Browse > Article
http://dx.doi.org/10.5352/JLS.2009.19.3.349

Comparison of Metabolic Fingerprintings between Biofilm and Aeration Tanks of RABC System for Food Wastewater Treatment  

Lee, Dong-Geun (Department of Pharmaceutical Engineering, Silla University)
Yoo, Ki-Hwan (Department of Pharmaceutical Engineering, Silla University)
Sung, Gi-Moon (Department of Pharmaceutical Engineering, Silla University)
Park, Seong-Joo (Department of Microbiology, Graduate School, Daejon University)
Lee, Jae-Hwa (Department of Pharmaceutical Engineering, Silla University)
Ha, Bae-Jin (Department of Pharmaceutical Engineering, Silla University)
Ha, Jong-Myung (Department of Pharmaceutical Engineering, Silla University)
Lee, Sang-Hyeon (Department of Pharmaceutical Engineering, Silla University)
Publication Information
Journal of Life Science / v.19, no.3, 2009 , pp. 349-355 More about this Journal
Abstract
Metabolic fingerprinting of microbial communities was investigated with Biolog GN2 plates using samples of biofilm and aeration tanks from an RABC (rotating activated Bacillus contactor) system - an advanced wastewater treatment system for the food wastewater of pig slaughterhouses. Aerobic and anaerobic results revealed the following four aspects. First, simple matching and pairs t-test of daily variation showed more defined qualitative and quantitative relatedness of active microbial communities than that of mere optical densities. Second, metabolic potentials were higher in biofilm than in aeration tanks (p<0.01), meaning higher activity of biofilm. Third, two aeration tanks showed the highest similarity (78%) and similar metabolic power (p=0.287). However, actively used carbon sources were different among samples, signifying change of active communities at each wastewater treatment step. Finally, aerobic and anaerobic metabolic fingerprinting patterns were different for the same samples representing activities of microaerophilic and/or anaerobic communities. These results suggest that daily variation and anaerobic incubation would help in the comparison of metabolic fingerprintings.
Keywords
Metabolic fingerprinting; biofilm; Biolog; RABC;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 R$\"{}$ling, W. F. M., B. M. van Breukelen, M. Braster, M. T. Goeltom, J. Groen, and H. W. van Verseveld. 2000. Analysis of microbial communities in a landfill leachate polluted aquifer using a new method for anaerobic physiological profiling and 16S rDNA based fingerprinting. Microb. Ecol. 40, 177-188
2 Ros, M., M. Goberna, J. A. Pascual, S. Klammer, and H. Insam. 2008. 16S rDNA analysis reveals low microbial diversity in community level physiological profile assays. J. Microbiol. Methods 72, 221-226   DOI   ScienceOn
3 Shishidoa, M., S. Kazunori, H. Yokoyama, N. Mommaa, and S. Miyashita. 2008. Changes in microbial communities in an apple orchard and its adjacent bush soil in response to season, land-use, and violet root rot infestation. Soil Biol. Biochem. 40, 1460-1473   DOI   ScienceOn
4 Viti, C. and L. Giovannetti. 2005. Characterization of cultivable heterotrophic bacterial communities in Cr-polluted and unpolluted soils using Biolog and ARDRA approaches. Appl. Soil Ecol. 28, 101-112   DOI   ScienceOn
5 Weber, K. P., J. A. Grove, M. Gehder, W. A. Anderson, and R. L. Legge. 2007. Data transformations in the analysis of community-level substrate utilization data from microplates. J. Microbiol. Methods 69, 461-469   DOI   ScienceOn
6 Choi, D. B., C.-B. Lee, and W.-S. Cha. 2005. Optimization for phosphorus removal by loess ball using Chromobacterium. J. Life Sci. 15, 584-589   DOI   ScienceOn
7 Garland, J. L. and A. L. Millis. 1991. Classification and characterization of heterotrophic microbial communities on the basis of patterns of community level sole carbon source utilization. Appl. Environ. Microbiol. 57, 2311-2359
8 Lee, D.-G., J.-H. Lee, S.-H. Lee, B. J. Ha, and J.-M. Ha. 2006. Efficiency of different disinfectants against biofilm on carbon steel pipe and carbon utilizing ability of biofilm. J. Life Sci. 16, 579-583   DOI   ScienceOn
9 Gamo, M. and T. Shoji. 1999. A method of profiling microbial communities based on a most-probable-number assay that uses BIOLOG plates and multiple sole carbon sources. Appl. Environ. Microbiol. 65, 4419-4424
10 Kim, E. H., Y. J. Jo, S. J. Park, G. S. Sin, S. B. Im, and J. G. Jeong. 2004. Advanced wastewater treatment process using rotating activated Bacillus contactor (RABC). J. Korean Soc. Wat. Qual. 20, 190-195
11 Mojica, K., D. Elseya, and M. J. Cooney. 2007. Quantitative analysis of biofilm EPS uronic acid content. J. Microbiol. Methods 71, 61-65   DOI   ScienceOn
12 Park, S. J., J. C. Yoon, K.-S. Shin, E. H. Kim, S. Yim, Y.-C. Cho, G. M. Sung, D.-G. Lee, S. B. Kim, D.-U. Lee, S.-H. Woo, and B. Koopman. 2007. Dominance of endospore- forming bacteria on a rotating activated Bacillus contactor biofilm for advanced wastewater treatment. J. Microbiol. 45, 113-121
13 Puigaguta, J., H. Salvadó, D. García, F. Granesc, and J. García. 2007. Comparison of microfauna communities in full scale subsurface flow constructed wetlands used as secondary and tertiary treatment. Wat. Res. 41, 1645-1652   DOI   ScienceOn
14 Anderson, S. A., C. H. Sissons, M. J. Coleman, and L. Wong. 2002. Application of carbon source utilization patterns to measure the metabolic similarity of complex dental plaque biofilm microcosms. Appl. Environ. Microbiol. 68, 5779-5783   DOI   ScienceOn