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http://dx.doi.org/10.5352/JLS.2008.18.8.1083

Analysis of the Diversity of Methicillin Resistant Staphylococcus aureus (MRSA) Strains Isolated from Diarrhea Patients in Busan Area  

Choi, Seong-Hwa (Busan Metropolitan City Institute of Health & Environment)
Park, Eun-Hee (Busan Metropolitan City Institute of Health & Environment)
Park, Yon-Koung (Busan Metropolitan City Institute of Health & Environment)
Kim, Joung-A (Busan Metropolitan City Institute of Health & Environment)
Kim, Nam-Ho (Busan Metropolitan City Institute of Health & Environment)
Lee, Young-Suk (Busan Metropolitan City Institute of Health & Environment)
Bin, Jae-Hun (Busan Metropolitan City Institute of Health & Environment)
Park, Ho-Kuk (Busan Metropolitan City Institute of Health & Environment)
Publication Information
Journal of Life Science / v.18, no.8, 2008 , pp. 1083-1089 More about this Journal
Abstract
We investigated the molecular epidemiological characteristic of methicillin resistant Staphylococcus aureus (MRSA) strains isolated from stool samples in Busan from 2004 to 2006. Among 142 isolates of S. aureus, 49 isolates (34.5%) were confirmed as MRSA. With the antimicrobial susceptibility tests, 37 isolates (75.5%) showed multiple resistance to more than 10 antibiotics, but all isolates were sensitive to vancomycin. All of MRSA had enterotoxin A in 30.6%, B 4.1%, C 8.3%, D, C/G, A 2.0% and None 51%. PFGE of SmaI-digested chromosomal DNA was performed on 49 sporadic MRSA isolates. Restriction fragment patterns consisted of 8 to 14 fragments ranged in size from 48.5 to 630.5 kbp. We could divided the isolates into 7 groups ($I{\sim}VII$) by analyzing PFGE patterns. Group I subdivided as 2 subgroups and 17 (34.7%) strains belong to the group I. Dendrogram of PFGE patterns showed that MRSA strains in Busan were heterogeneous but we could find out minor homogeneity in hospital.
Keywords
Methicillin resistant Staphylococcus aureus (MRSA); antimicrobial susceptibility; mecA gene; enterotoxin; PFGE;
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1 Kim, B. S., Y. S. Lee, J. I. Yoo, J. K. Lee, J. O. Cha and E. S. Shin. 2001. Analysis of mec regulation gene and PFGE on Staphylococcus aureus isolates from tertiary hospitals in Korea. The Report of NIH 38, 43-51
2 Woodford, N. PCR for the detection of antibiotic resistance genes Laboratory of Hospital infections SOP No. L-4109, 3-98
3 Cha, J. O., J. K. Lee, Y. H. Jung, J. I. Yoo, Y. K. Park, B. S. Kim and Y. S. Lee. 2006. Molecular analysis of Satapylococcus aureus isolates associated with staphylcococcal food poisoning in South Korea. J. Appli. Micro. 101, 864-871   DOI   ScienceOn
4 Chong, Y. M., K. S. Lee, Y. S. Park, D. S. Jeon, M. H. Lee and M. Y. Kim. 1998. Korean nationwide surveillance of antimicrobial resistance of bacteria in 1997. Yonsei Med. J. 39, 569-577   DOI   ScienceOn
5 Clinical and Laboratory Standards Institute. 2006. Performance Standards for Antimicrobial Disk Susceptibility Tests. pp. 1-28, Approved Standard-Ninth Edition, Clinical and Laboratory Standards Institute, USA
6 Jorgensen, J. H. 1991. Mechanisims of methicillin resistnace in staphylococcus aureus and method for laboratory detection. Infect. Control Hosp. Epidemiol. 12, 14-19   DOI   ScienceOn
7 Park, E. H., G. Y. Jeoung. 2004. Distribution of enterotoxin type in Stapylococcus aurues isolates from stool samples. Rep. Busan. Inst. Heatlth & Environ. 14, 28-43
8 Park, S. J., Y. S. Jeoung and S. Y. Lee. 1977. Antimicrobial susceptibility study of isolates from clinic examination. Korean J. Pathol. 11, 119-125
9 Han, S. J., P. M. Jung, H. G. Kim, E. H. Hwang and I. W. Seong. 1999. Multiple intestinal ulcerations and perforations secondary to methicillin-resistant Staphylococcus aureus enteritis in infants. J. Pediatr. Surg. 34, 381-386   DOI   ScienceOn
10 Park, S. G., Y. O. Hwang, J. H. Jung and K. M. Lee. 2001. Biological characteristics of Staphylococcus aureus isolated from food-borne patients in Seoul. J. Fd. Hyg. Safety 16, 159-167
11 Tenover, F. C., R. V. Arbeit and P. A. Goering. 1995. Interpreting chromosomal DNA restriction patterns produced by pulsed- field gel electrophoresis: criteria for bacterial strain typing. J. Clin. Microb. 33, 2233-2239
12 Lee, Y. S., H. B. Kim, J. I. Yoo, S. J. Yang. C. M. Sa, Y. H. Choi and B. S. Kim. 1999. Molecular epidemiology of methicillin resistant Staphylococcus aureus in Korea. The Report of NIH 36, 67-76
13 Haley, R. W., N. B. Cushin, F. C. Tenober, T. L. Bannerman, D. Dryer, J. Ross, P. J. Sanchez and J. D. Siegel. 1995. Eradication of endemic methicillin-resistant Staphylococcus aureus infections from a neonatal intensive care unit. J. infect Dis. 171, 614-624   DOI   ScienceOn
14 Hiramatsu, K., T. Ito, H. Hanaki. 1999. Mechanism of methicillin and vancomycin resistance in Staphylococcus aureus. Balliere's Clin. Infect Dis. 5, 221-242
15 Lee, Y. S., J. O. Cha, Y. H. Jung, Y. H. Choi, Y. S. Yang and H. B. Kim. 2000. Molecular epidemiology study on Satpylococcus aureus isolates from blood specimen in Korea. The Report of NIH 37, 53-60
16 Lencastre, D. H., M. Chung, H. Westh. 2000. Archaic Strains of methicillin-resistant Staphylococcus aureus: Molecular and microbiological properties of isolates from the 1960's in Denmark. Microb. Drug Resist 6, 1-10   DOI   ScienceOn
17 Hartstein, A. l., M. A. Dennys, V. H. Morth-land, A. M. LeMonte, M. A. Pfaller. 1995. Control of methicillin-resistant Staphylococcus aureus in a hospital and an intensive care unit. Infect Control Hosp. Epidemiol. 16, 405-411   DOI   ScienceOn
18 Moon, J. Y., E. J. Lee and Y. B. Kim. 2004. Rapid detection of methicillin-resistant Staphylococcus aureus by multiplex PCR. J. Bac. and Viro. 34, 91-100
19 Francis, A. W. 1995. Staphylococcus aureus (including toxic shock syndrom). pp. 1754-1755, Mandell Douglas and Bennetts Principles and Infections Disease. 4th eds., Churchill Livingstone
20 Lee, K. W., E. C. Kim, W. G. Lee, S. H. Jeong, Y. J. Park, D. G. Yong, J. H. Song and H. K. Lee. 2006. Develpment of control system of antimicorbial resistence in clinical isolates of bacteria. Annual Report of NARMP 4, 111-145
21 Jeoung, H. Y., S. J. Jang, S. D. Lee, S. H. Park, C. S. Min, S. Y. Lee, K. H. Lee, J. E. Lee, M. S. Lee and K. W. Lee. 2002. Monitoring on the bacterial resistance to antibiotics. The Annual Report of KFDA 6, 222-229
22 Chambers, H. F. 1997. Methicillin resistance in staphylococci- molecular and biochemical basis and clinical implications. Clin. Microbiol. Rev. 10, 781-791