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http://dx.doi.org/10.5352/JLS.2007.17.1.076

Inhibitory Effect of Methanol Extracts and Solvent Fractions from Meju on Mutagenicity and Growth of Human Cancer Cells  

Lim, Sun-Young (Division of Marine Environment & Bioscience, Korea Maritime University)
Park, Kun-Young (Department of Food Science and Nutrition, Pusan National University)
Lee, Sook-Hee (Department of Food Science and Nutrition, Pusan National University)
Choi, Jae-Soo (Division of Food and Life Science, Pukyung National University)
Publication Information
Journal of Life Science / v.17, no.1, 2007 , pp. 76-81 More about this Journal
Abstract
Inhibitory effects of methanol extracts and several solvent fractions from meju on mutagenicity in vitro genotoxicity (SOS chromotest) and growth of human cancer cells (AGS gastric adenocarcinoma and Hep 3B hepatocellular cancinoma cells) were studied. The treatment of meju methanol extracts $(100{\mu}g/assay)$ to SOS chromotest system inhibited N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) induced mutagenicity by 36%. However, the ethylacetate and dichloromethane fractions from meju methanol extracts showed the stronger antimutagenic effects (91% and 91%, respectively) in SOS chromotest. In sulforhodamine B (SRB) assay, the treatments of ethylacetate and dichloromethane fractions (2 mg/assay) significantly inhibited the growth of AGS and Hep 3B cancer cells by 64% and 71%, respectively. These results indicated that meju had inhibitor)r effects on MNNG in SOS mutagenic system and growth of human cancer cells, suggesting that its antimutagenic effect may be relative to activity of doenjang.
Keywords
Meju; SOS chromotest; antimutagnicity; cancer cells; sulforhodamine B (SRB) assay;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Skehan, P., R. Storeng, D. Scudiero, A. Monks, J. McMahon, D. Vistica, J. T. Warren, H. Bokesch, S. Kenney and M. R. Boyd. 1990. New colorimetric cytotoxity assay for anticancer-drug screening. J. Natl. Cancer Inst. 82, 1107-1112   DOI
2 Song, S. K., K. H. Kim and H. S. Kim. 2001. Cytotoxic effects and compounds of lipid fractions from soybean products on cancer cells. J. Kor. Soc. Food Sci. Nutr. 30, 1266-1271   과학기술학회마을
3 Park, K. Y., S. Y. Lim and S. H. Rhee. 1997. Antimutagenic and anticarcinogenic effects of doenjang. J. Kor. Assoc. Cancer Prevention 1, 99-107
4 Park, S. K., K. I. Seo, S. H, Choi, J. S. Moon and Y. H. Lee. 2000. Quality assessment of commercial doenjang prepared by traditional method. J. Kor. Soc. Food Nutr. 29, 211-217   과학기술학회마을
5 Park, Y. W., K. S. Hwang, S. W. Park and S. H. Kim. 1959. A study on soybean past (part III); Change of free amino acid contents during aging process. Kwayonshuibo 4, 31-36
6 Peterson, G. 1995. Evaluation of the biochemical target of genistein in tumor cells. J. Nutr. 125, 784S-789S
7 Quillardet, P., C. D. Bellecomide and M. Hofnung. 1985. The SOS chromotest. a colorimetric bacterial assay for genotoxins: validation study with 83 compunds. Mutat. Res. 147, 79-95   DOI   ScienceOn
8 Quillardet, P. and M. Hofnung. 1985. The SOS chromotest. a colorimetric bacterial assay for genotoxins. Mutat. Res. 147, 65-78   DOI   ScienceOn
9 Quillardet, P., O. D. Huisman, R. Ari and M, Hofnung. 1982. SOS chromotest. a direct assay of induction of an SOS function in Escherichia coli K-12 to measure genotoxicity. Proc. Natl. Acad. Sci. USA 79, 5971-5975   DOI
10 Rueff, J. A., L. H. Borba, T. Chaveca, M. I. Gomes and M. Halpern. 1986. Genetic toxicology of flavonoids : The role of metabolic conditions in the induction of reverse mutation. SOS function and sister-chromatid exchanges. Mutagenesis 1, 179-183   DOI   ScienceOn
11 Lim, S. Y., S. H. Rhee and K. Y. Park. 2005. Effect of solvent fractions from methanol extract of doenjang on inhibition of growth and DNA synthesis of human cancer cells. Kor. J. Life Sci. 15, 685-691   과학기술학회마을   DOI   ScienceOn
12 Shin, Z. I., C. W. Ahn, H. S. Nam, H. J. Lee, H. J. Lee and T. H. Moon. 1995. Fractionation of angiotensin converting enzyme (ACE) inhibitory peptides from soybean paste. Kor. J. Food Sci. Technol. 27, 230-234   과학기술학회마을
13 Lee, J. S. and H. S. Cheigh. 1997. Antioxidative characteristics of isolated crude phenolics from soybean fermented foods (doenjang). J. Kor. Sco. Food Sci. Nutr. 26, 376-382   과학기술학회마을
14 Lim, S. Y., K. Y. Park and S. H. Rhee. 2004. Inhibitory effect of methanol extracts and solvent fractions from doenjang on mutagenicity using in vitro SOS chromotest and in vivo Drosophila mutagenic system. J. Kor. Soc. Food Sci. Nutr. 33, 1432-1438   DOI   ScienceOn
15 Monks, A., D. Scudiero, P. Skehan, R. Shoemarker, K. Paull, D. Vistica, C. Hose, J. Langley, P. Cronise, A. Vaigro-Wolff, M. Gray-Goodrich, H. Campbell, J. Mayo and M. Boyd. 1991. Feasiblity of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines. J. Natl. Cnacer lnst. 83, 757-766   DOI
16 Ohta, T., N. Nakamura, M. Moriya. T. Shirai and T. Kada. 1984. The SOS function-inducing activity of chemical mutagens in Escherichia coli. Muta. Res. 131, 101-109   DOI
17 Park, K. J. and K. J. Kim. 1970. Studies on the manufacturing of soysauce (part I). In Research Report. Central Industrial Research Institute. Vol. 20, p 89-93
18 Baik, C.W. and S. S. Ham. 1990. Antimutagenic effects of browning products reacted with polyphenol oxidase extracted from apple by using SOS chromotest. Kor. J. Food Sci. Technol. 22, 618-624   과학기술학회마을
19 Park, K. Y., S. H. Moon, H. S. Baik and H. S. Cheigh. 1990. Antimutagenic effect of doenjang (Korean fermented soy paste) toward aflatoxin $B_1$. J. Kor. Sco. Food Nutr. 19, 156-162   과학기술학회마을
20 Park, K. Y., S. H. Moon and S. H. Rhee. 1995. Antimutagenic effect of doenjang (Korean soy paste) - Inhibitory effect of doenjang stew and soup on the mutagenicity induced by aflatoxin $B_1$. Environ. Muta. Carinogen. 14, 145-1521
21 Choi, G. S., S. Y. Lim and J. S. Choi. 1998. Antioxidant and nitrile scavenging effect of soybean, meju and doenjang, Kor. J. Life Sci. 8, 473-478   과학기술학회마을
22 Han, Y. S. and B. D. Park. 1957. Studies on the manufacturing of soysauce (part I); 'Aspergillus oryzae from ordinary Meju on Gokja' In Research Report. Central Industrial Research Institute. Vol. 7, p 51-56
23 Shin, S. H., E. C. Jhee, N. S. Rapp, I. S. Hong, S. H. Chang and D. J. Seel. 1989. Mutagenicity and antimutagenicity of meju, hot sauce and other Korean foods by salmonella/mammalian-microsome test. : Abstract p301 preseted at 5th Federation of Asian and Oceanian Biochemists, August, 13-18, Seoul, Korea
24 Kim, S. Y., K. D. Lee, M. H. Kim and C. K. Yoo. 1968. Investigation on amino acid contents of soybean paste. Modern Medicine 9, 183-190
25 Lee, C. H. 1973. Studies on the amino acid composition of Korean fermented soysause, meju products and the evaluation of the protein quality. Kor. J. Food Sci. Technol. 5, 210-215   과학기술학회마을
26 Choi, S. Y., M. J. Cheigh, J. J. Lee, H. J. Kim, S. S. Hong, K. S. Chung and B. K. Lee. 1999. Growth suppression effect traditional fermented soybean paste on various tumor cells. J. Kor. Soc. Food Sci. Nutr. 28, 458-463   과학기술학회마을
27 Lim, S. Y., K. Y. Park and S. H. Rhee. 1999. Anticancer effects of doenjang in in vitro sulforhodamine B (SRB) assay. J. Kor. Soc. Food Sci. Nutr. 28, 240-245   과학기술학회마을