[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5352/JLS.2006.16.2.192

The Effects of Diesel Exhaust Particles on the Alveolar Macrophages for Inducible Nitric Oxide Synthase Induction and Nitric Oxide with Nitrotyrosilated-protein Formation

Lim Young (Catholic University College of Medicine)
Choe Myung-Ok (Catholic University College of Medicine)
Lee Kweon-Haeng (Catholic University College of Medicine)
Kim Kyung-A (Catholic University College of Medicine)
Kim Kil-Soo (Kyungpook national University College of Veterinary Medicine)
Lee Myoung-Heon (National Veterinary Research and Quarantine Service)
Li Tian-Zhu (Konkuk University College of Veterinary Medicine)
Lee Soo-Jin (Konkuk University College of Veterinary Medicine)
Choe Nong-Hoon (Konkuk University College of Veterinary Medicine)

Publication Information

Journal of Life Science / v.16, no.2, 2006 , pp. 192-198 More about this Journal

Abstract

Epidemiological studies have demonstrated an association between exposure to diesel exhaust particles (DEP) and adverse cardiopulmonary effects. Despite the epidemiological proof, the pathogenesis of DEP-related pulmonary diseases remain poorly understood. So, comprehensive in vivo and in vitro researches are required to know the effects of DEP on diverse lung diseases. Alveolar macrophages (AM) and airway epithelial cells are known as important cellular targets in DEP-induced lung diseases. Other studies have shown that nitric oxide (NO) is involved in particle matter induced lung injury. The present study was undertaken to determine whether DEP has an synergistic effects on lipopolysaccharide (LPS)-induced NO formation and inducible nitric oxide synthase (iNOS) with nitrotyrosilated-protein formation in cultured primary alveolar macrophages. The formation of NO was determined through the Griess reaction in the cultured medium and iNOS with nitrotyrosilated-proteins are analyzed by immunohistochemical staining and Western analysis. The results indicate that DEP exposure does not induce NO formation by itself, however DEP showed significant synergistic effects on LPS-induced NO formation. So, our results suggest that DEP inhalation could aggravate inflammatory lung disease through NO formation.

Keywords

Diesel exhaust particles; alveolar macrophages; nitric oxide; nitrotyrosilated-protein; lipopolysaccharide;

Citations & Related Records

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