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http://dx.doi.org/10.5352/JLS.2006.16.1.095

The Protective Effects of Salicornia herbacea L. against Liver Toxicity  

Ha, Bae-Jin (Department of Bioscience and Biotechnology, Silla University)
Lee, Sang-Hun (Department of Bioscience and Biotechnology, Silla University)
Publication Information
Journal of Life Science / v.16, no.1, 2006 , pp. 95-100 More about this Journal
Abstract
This study was carried out to investigate the effects of Salicornia herbacea L. (SH) on carbon tetrachloride $(CCl_4)-induced$ hepatotoxicity. Sprague-Dawley rats were intraperitoneally administered the SH at 100 mg/kg per day for two weeks. Then single dose of $CCl_4$ (3.3 ml/kg) was injected into rats. Twelve hours later, they were anesthesized with ether and dissected. $SH-CCl_4-administered$ group showed $65.56\%\;and\;59.04\%$ of inhibitory effects in aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities, compared to $CCl_4-treated$ group (p<0.05). Malonedialdehyde (MDA) levels of $SH-CCl_4-administered$ group in liver homogenate and mitochondria were significantly inhibited by $53.74\%,\;89.86\%$, and respectively, compared to $CCl_4-treated$ group (p<0.05). Superoxide dismutase (SOD) activities of $SH-CCl_4-administered$ group in liver homogenate and mitochondria were significantly inhibited by $42.51\%,\;and\;38.42\%$, respectively, compared to $CCl_4-treated$ group (p<0.05). The histological examinations showed that the liver cell necrosis and centrilobular congestive aggregation induced by $CCl_4$ were clearly eliminated by the administration of SH. These results suggest that SH could have the protective effects against hepatotoxicity.
Keywords
Salicornia herbacea; hepatotoxicity; MDA; SOD;
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1 Jo, Y.C., Ahn, J. H., Chon, S.M., Lee, K.S., Bae, T. J., and Kang D.S. 2002, Studies on pharmacological effects of glasswort(salicornia herbacea L.), Korean J. Med. Crop Sci. 10, 93-99
2 Shimizu K, 2000, Effects of salt treatments on the production and chemical composition of salt wort (Salicornia herbacea L.), rhodesgrass and alfalfa, Jpn J. Trop. Agr. 44, 61-67
3 이창복, 1988, 대한식물도감, 향문사, 317
4 Stewart, R. D., Boettner, E. A., Southworth, R. R., et al., 1963, Acute carbon tetrachloride intoxication. JAMA, 994-997
5 이홍규, 1985, 대한식물도감, 향문사, 990
6 최원경: Streptozotocin 유발 당뇨쥐에 있어서 항산화적 방어기구 및 metallothionein 합성에 미치는 비타민 E의 영향. 효성여자대학교 박사학위논문
7 Recknigel, R.O., 1967, Carbon tetrachloride hepatotoxicity. Pharmacol. Rev. 19, 45-208
8 Freeman BA, Crapo JD.,1982, Biology of disease: Free radicals and tissue injury. Lab. Invest. 47, 412
9 Bompart G. J., Prevot D. S., Bascands, J. S., 1990, Rapid automated analysis of glutathione reductase, peroxidase and Stranaferase activity : Application to Iisplatin-induced toxicity, Clin. Biochem. 23, 501-504   DOI   ScienceOn
10 Choon-Sik Jeong, Ki-Wha Jung and Jeong-Suk Jeong. 1999. Hepatoprotective Effect of Subfractions of Carthamus tinctorius L. Semen on the Reversal of Biotransformation Enzyme Activities in $CCl_4-induced$ Hepatotoxic Rats. J. Fd Hyg. Safety. 14(2), 172-178
11 Halliwell B.: Oxidants and human disease: Some new concepts. FASEJ 1(358), 1987
12 Kulesar-Gergely, J. and A. Kluesar, 1997, Studies in the effects of ursodeoxycholic acid in rats with actue carbon tetrachloride injury. Arzneim-Forsch./Drus Res. 47, 659-661
13 Lee J.T. and An B.J., 2002, Deffection of physical activity of salicornia herbacea, Kor. J. Herbology. 17(2), 61-69
14 Logani, M.K. and Davis, R.E., 1979, Lipid peroxidation, Lipids 15, 485   DOI   ScienceOn
15 Lowry, O. H., Rosenbrough. N. J., Farr, A. S. and Randall, R. J. 1951. Protein Measurenment with Folin phenol reagent. J. Biol. Chem. 193, 256-261
16 Park, S. H. and Kim, K.S., 2004, Isolation and Identification of Antioxidant Flavonoids from Salicornia herbacea L., J. Korean Soc. Appl. Biol. Chem, 47(1), 120-123   과학기술학회마을
17 Pecknagel, R.D. and Glende, E. A., 1973, carbon tetrachloride hepatotoxicity, CRC Crit. Rev. Toxicol. 2, 263-297   DOI
18 Plaa, G. L. and Hewitt, W. R., 1982, Principles and Methods of Toxicology, Raben Press 407-445
19 Pohl, L., Schulick, R. and George J., 1968, Reductive oxygenation mechanism of metabolism for carbon tetrachloride to phosgene by cytochrome P450. Mol. Pharmacol. 25, 318-324
20 Beauchamp, C. and Fridovich, I. 1971. Superoxide dismutase : improved assays and an assay applicable to acrylamide gel. Anal. Biochem. 44, 276-287   DOI   ScienceOn
21 Tainer, J. A., Getzoff, E. D., Richardson, J. S., and Richardson, D. C. 1983. Structure and mechanism of Cu, Zn, superoxide dismutase. Nature 306, 274-287   DOI   ScienceOn
22 Takaharu Nomura and Kiyonori Yamaoka. 1999. LOW-DOSE ${\gamma}-RAY IRRADIATION$,REDUCES OXIDATIVE DAMAGE INDUCED BY CCl4 IN MOUSE LIVER. Free Radical Biology & Medicine. 27(11/12), 1324-1333   DOI   ScienceOn
23 Ohkawa, A. and Ohishi, N. and Yagi, K. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95, 351-358   DOI   ScienceOn
24 Rosen, D. R., etal. 1993. Mutations with familial amyotrophic lateral sclerosis. Nature 362, 59-62   DOI   ScienceOn
25 Min J.G., Lee D.S., Kim T.J., Park, J.H., Cho T.Y., Park D.I., 2002, Chemical composition of Salicornia herbacea L., J. Food Sci. Nutr. 7, 105-107   DOI   ScienceOn