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http://dx.doi.org/10.5423/RPD.2010.16.2.136

Suppression Effect on Soft-rot by Bacteriocin-producing Avirulent Pectobacterium carotovorum subsp. carotovorum Pcc21-M15  

Roh, Eun-Jung (Microbial Safety Division, National Academy of Agricultural Science, RDA)
Lee, Seung-Don (Research Policy Bureau, RDA)
Heu, Sung-Gi (Microbial Safety Division, National Academy of Agricultural Science, RDA)
Publication Information
Research in Plant Disease / v.16, no.2, 2010 , pp. 136-140 More about this Journal
Abstract
Pectobacterium carotovorum subsp. carotovorum causes soft rot disease in diverse plants. Carocin D is bacteriocin that is produced by Pectobacterium carotovorum subsp. carotovorum Pcc21 strain. Nonpathogenic mutant P. carotovorum subsp. carotovorum Pcc21-M15 strain was obtained by mutagenesis with Tn5 insertion and screened pathogenesity. P. carotovorum subsp. carotovorum Pcc21-M15 and E. coli (pRG3431), carocin D gene-transformed E. coli, produce carocin D against P. carotovorum subsp. carotovorum Pcc3. Pathogenic P. carotovorum subsp. carotovorum Pcc3 and mixture with Pcc21-M15 or E. coli (pRG3431) were treated with lettuces. Pcc21-M15 and E. coli (pRG3431) effectively suppressed the development of soft-rot disease. While symptoms in 90% of Pcc3-treated lettuces were observed after 3 days, only 25% of Pcc3 and Pcc21-M15-treated lettuces were observed to be infected after 6 days. These results suggest that the nonpathogenic strain P. carotovorum subsp. carotovorum. Pcc21-M15 and E. coli (pRG3431) are effective to soft-rot disease suppression.
Keywords
Bacteriocin; Carocin D; Nonpathogenic mutant; Pectobacterium; Soft rot;
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