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http://dx.doi.org/10.7856/kjcls.2016.27.3.437

Single Oral Dose Toxicity Test and Four Weeks Repeated Oral Dose Determination Test of Crude Antifungal Compounds Produced by Bacillus subtilis SN7 in Rats  

Chang, Hae-Choon (Dept. of Food and Nutrition, Chosun University)
Koh, Sang-Bum (Korea Testing & Research Institute, Health Care Research Laboratory)
Lee, Jae-Joon (Dept. of Food and Nutrition, Chosun University)
Publication Information
The Korean Journal of Community Living Science / v.27, no.3, 2016 , pp. 437-449 More about this Journal
Abstract
To provide information on the safety of crude antifungal compounds produced by Bacillus subtilus SN7 isolated from Meju, we carried out an acute (single) oral dose toxicity test and 4 week repeated oral dose determination test on crude antifungal compounds in male and female Sprague Dawley rats. In the acute toxicity test, rats were treated with crude antifungal compounds produced by Bacillus subtilus SN7 orally at increasing dose levels (500, 1,000, and 2,000 mg/kg) and observed for 2 weeks. In the repeated-dose 28-day oral dose determination study, rats were orally administered doses of 500, 1,000, and 2,000 mg/kg daily for 4 weeks. There were no test article-related deaths or abnormal clinical signs in the two studies. In the 4 week repeated oral dose determination test, there were also no significant differences in clinical signs, body and organ weight changes, or any other hematological and biochemical parameters between the control and treated groups. The results suggest that the crude antifungal compounds produced by Bacillus subtilus SN7 up to a dosage level of 2,000 mg/kg are not toxic in male and female rats.
Keywords
Bacillus subtilus SN7; crude antifungal compounds; acute toxicity; safety; 4 week repeated dose determination; safety;
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Times Cited By KSCI : 7  (Citation Analysis)
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