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Karyotype Analysis and rDNA Physical Mapping in Rye (Secale cereale L.)  

Lee, Joon Soo (Research Institute of Technology R&D Headquarter, KGC)
Seo, Bong Bo (Department of Biology, College of Natural Science, Kyungpook National University)
Kim, Min (Department of Horticultural Science, College of Life & Environmental Sciences, Daegu University)
Publication Information
Korean Journal of Breeding Science / v.42, no.2, 2010 , pp. 163-168 More about this Journal
Abstract
This study was carried out to determine the chromosomal localization of the 5S and 18S-26S ribosomal DNA(rDNA) genes by means of fluorescence in situ hybridization(FISH) techniques, and the constitutive heterochromatin detected by means of Gimsa C-banding technique in rye(Secale cereale L.). The somatic chromosomes number was 2n=14. The karyotype consists of four pairs of metacentrics(chromosomes 1, 2, 3, and 7) and three pairs of submetacentrics(chromosomes 4, 5, and 6). Secondary constrictions appeared in the short arm of chromosome 1. The 5S rDNA genes have been located on two pairs of chromosomes 1 and 5, and 18S-26S rDNAs genes have been located on one pair of chromosome 1. 5S rDNA genes were detected on the distal region of the secondary constrictions in nucleolus organizer regions(NOR) in chromosome 1, and other detected on the intercalary region in the short arm of chromosome 5.
Keywords
Rye; C-banding; rDNA; FISH;
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