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http://dx.doi.org/10.7314/APJCP.2014.15.24.10585

Clinicopathological Significance of BRCA1 Promoter Hypermethylation in Thai Breast Cancer Patients  

Saelee, Pensri (Research Division, National Cancer Institute)
Chaiwerawattana, Arkom (Surgery Division, National Cancer Institute)
Ogawa, Kumiko (Pathology Division, National Institute of Health Sciences)
Cho, Young-Man (Pathology Division, National Institute of Health Sciences)
Tiwawech, Danai (Research Division, National Cancer Institute)
Suktangman, Vimol (Pathology Division, National Institute of Health Sciences)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.24, 2015 , pp. 10585-10589 More about this Journal
Abstract
Breast cancer susceptibility gene 1 (BRCA1), mapped on chromosome 17q21, is implicated in the mechanisms of cellular DNA repair. Inactivation of this gene is involved in the development of many human cancers, including breast cancer. This study aimed to investigate the prognostic value of BRCA1 promoter hypermethylation and expression in breast cancer cases. Sixty-one breast cancers were examined for BRCA1 hypermethylation by methylation-specific polymerase chain reaction (PCR), and 45 paired normal breast tissues were analyzed for altered BRCA1 mRNA levels by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). Aberrant methylation status in BRCA1 was detected in 15 of 61 cases (24.6%), while reduced expression was found in 7 of 45 (15.6%). BRCA1 hypermethylation was statistically associated with tumor grade III (p=0.04), a high frequency of stage IIB (p=0.02), and triple-negative phenotype (OR= 3.64, 95%CI =1.1-12.3, p=0.03). Our findings indicated that BRCA1 promoter hypermethylation is a useful prognostic marker for breast cancer.
Keywords
Breast cancer; BRCA1; DNA methylation; methylation-specific PCR; gene expression;
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