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http://dx.doi.org/10.7314/APJCP.2014.15.2.643

Effects of Tissue Factor, PAR-2 and MMP-9 Expression on Human Breast Cancer Cell Line MCF-7 Invasion  

Lin, Zeng-Mao (Breast Disease Center, Peking University First Hospital)
Zhao, Jian-Xin (Breast Disease Center, Peking University First Hospital)
Duan, Xue-Ning (Breast Disease Center, Peking University First Hospital)
Zhang, Lan-Bo (Breast Disease Center, Peking University First Hospital)
Ye, Jing-Ming (Breast Disease Center, Peking University First Hospital)
Xu, Ling (Breast Disease Center, Peking University First Hospital)
Liu, Yin-Hua (Breast Disease Center, Peking University First Hospital)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.2, 2014 , pp. 643-646 More about this Journal
Abstract
Objective: This study aimed to explore the expression of tissue factor (TF), protease activated receptor-2 (PAR-2), and matrix metalloproteinase-9 (MMP-9) in the MCF-7 breast cancer cell line and influence on invasiveness. Methods: Stable MCF-7 cells transfected with TF cDNA and with TF ShRNA were established. TF, PAR-2, and MMP-9 protein expression was analyzed using indirect immunofluorescence and invasiveness was evaluated using a cell invasion test. Effects of an exogenous PAR-2 agonist were also examined. Results: TF protein expression significantly differed between the TF cDNA and TF ShRNA groups. MMP-9 protein expression was significantly correlated with TF protein expression, but PAR-2 protein expression was unaffected. The PAR-2 agonist significantly enhanced MMP-9 expression and slightly increased TF and PAR-2 expression in the TF ShRNA group, but did not significantly affect protein expression in MCF-7 cells transfected with TF cDNA. TF and MMP-9 expression was positively correlated with the invasiveness of tumor cells. Conclusion: TF, PAR-2, and MMP-9 affect invasiveness of MCF-7 cells. TF may increase MMP-9 expression by activating PAR-2.
Keywords
Breast cancer; tissue factor; protease activated receptor-2; matrix metalloproteinase-9;
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