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http://dx.doi.org/10.7314/APJCP.2014.15.1.475

Apoptosis Induction in MV4-11 and K562 Human Leukemic Cells by Pereskia sacharosa (Cactaceae) Leaf Crude Extract  

Asmaa, Mat Jusoh Siti (Department of Haematology, School of Medical Sciences, Universiti Sains Malaysia)
Al-Jamal, Hamid Ali Nagi (Department of Haematology, School of Medical Sciences, Universiti Sains Malaysia)
Ang, Cheng Yong (Department of Haematology, School of Medical Sciences, Universiti Sains Malaysia)
Asan, Jamaruddin Mat (Department of Immunology, School of Medical Sciences, Universiti Sains Malaysia)
Seeni, Azman (Advanced Medical and Dental Institute, Universiti Sains Malaysia)
Johan, Muhammad Farid (Department of Haematology, School of Medical Sciences, Universiti Sains Malaysia)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.1, 2014 , pp. 475-481 More about this Journal
Abstract
Background: Pereskia sacharosa is a genus of cacti widely used in folk medicine for cancer-related treatment. Anti-proliferative effects have been studied in recent years against colon, breast, cervical and lung cancer cell lines, with promising results. We here extended study of anti-proliferative effects to a blood malignancy, leukemia. Materials and Methods: Two leukemic cell lines, MV4-11 (acute myeloid leukemia) and K562 (chronic myeloid leukemia), were studied. $IC_{50}$ concentrations were determined and apoptosis and cell cycle regulation were studied by flow cytometric analysis. The expression of apoptosis and cell-cycle related regulatory proteins was assessed by Western blotting. Results: P sacharosa inhibited growth of MV4-11 and K562 cells in a dose-dependent manner. The mode of cell death was via induction of intrinsic apoptotic pathways and cell cycle arrest. There was profound up-regulation of cytochrome c, caspases, p21 and p53 expression and repression of Akt and Bcl-2 expression in treated cells. Conclusions: These results suggest that P sacharosa induces leukemic cell death via apoptosis induction and changes in cell cycle checkpoint, thus deserves further study for anti-leukemic potential.
Keywords
Pereskia sacharosa; leukemia; intrinsic pathway; cell cycle arrest; FLT3-ITD; BCR-ABL;
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