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http://dx.doi.org/10.7314/APJCP.2013.14.5.3109

Anti-tumor Efficacy of a Hepatocellular Carcinoma Vaccine Based on Dendritic Cells Combined with Tumor-derived Autophagosomes in Murine Models

Su, Shu (Cancer Research and Biotherapy Center, the Affiliated Nanjing Second Hospital, Medical School of Southeast University)
Zhou, Hao (Department of Microbiology and Immunology, Medical School of Southeast University)
Xue, Meng (Department of Microbiology and Immunology, Medical School of Southeast University)
Liu, Jing-Yu (Department of Microbiology and Immunology, Medical School of Southeast University)
Ding, Lei (Department of Microbiology and Immunology, Medical School of Southeast University)
Cao, Meng (Department of Microbiology and Immunology, Medical School of Southeast University)
Zhou, Zhen-Xian (Cancer Research and Biotherapy Center, the Affiliated Nanjing Second Hospital, Medical School of Southeast University)
Hu, Hong-Min (Cancer Research and Biotherapy Center, the Affiliated Nanjing Second Hospital, Medical School of Southeast University)
Wang, Li-Xin (Cancer Research and Biotherapy Center, the Affiliated Nanjing Second Hospital, Medical School of Southeast University)

Publication Information

Asian Pacific Journal of Cancer Prevention / v.14, no.5, 2013 , pp. 3109-3116 More about this Journal

Abstract

The majority of hepatocellular carcinoma (HCC) patients have a poor prognosis with current therapies, and new approaches are urgently needed. We have developed a novel therapeutic cancer vaccine platform based on tumor cell derived autophagosomes (DRibbles) for cancer immunotherapy. We here evaluated the effectiveness of DRibbles-pulsed dendritic cell (DC) immunization to induce anti-tumor immunity in BALB/c mouse HCC and humanized HCC mouse models generated by transplantation of human HCC cells (HepG2) into BALB/c-nu mice. DRibbles were enriched from H22 or BNL cells, BALB/c-derived HCC cell lines, by inducing autophagy and blocking protein degradation. DRibbles-pulsed DC immunization induced a specific T cell response against HCC and resulted in significant inhibition of tumor growth compared to mice treated with DCs alone. Antitumor efficacy of the DCs-DRibbles vaccine was also demonstrated in a humanized HCC mouse model. The results indicated that HCC/DRibbles-pulsed DCs immunotherapy might be useful for suppressing the growth of residual tumors after primary therapy of human HCC.

Keywords

Hepatocellular carcinoma; dendritic cells; autophagy; immunotherapy; tumor antigen; cross-presentation;

Citations & Related Records

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