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http://dx.doi.org/10.7314/APJCP.2013.14.4.2295

Caveolin-1, Through its Ability to Negatively Regulate TLR4, is a Crucial Determinant of MAPK Activation in LPS-challenged Mammary Epithelial Cells  

Wang, Xiao-Xi (College of Life Sciences, Liaoning Normal University)
Wu, Zheng (College of Life Sciences, Liaoning Normal University)
Huang, Hui-Fang (College of Life Sciences, Liaoning Normal University)
Han, Chao (The First Affiliated Hospital, Dalian Medical University)
Zou, Wei (College of Life Sciences, Liaoning Normal University)
Liu, Jing (The First Affiliated Hospital, Dalian Medical University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.14, no.4, 2013 , pp. 2295-2299 More about this Journal
Abstract
Background: To explore the role of caveolin-1(CAV-1) gene silencing on MAPK activation in lipopolysaccharide (LPS)-challenged human mammary epithelial cells. Methods: We established a MCF-10ACE of CAV-1 gene silencing from human mammary epithelial cell line MCF-10A by RNAi technology. DNA Microarray were used to detect the expression of inflammation-associated genes in MCF10ACE. Western blotting was used to examine the activation of MAPK in lipopolysaccharide(LPS)-challenged MCF-10A and MCF-10ACE. Moreover, immunofluorescence and Western bloting were performed to detect the co-localization of CAV-1 and toll-like receptor 4 (TLR4) in human mammary epithelial cells. Results: MCF-10ACE exhibited significant increases in inflammation-associated gene expression, especially IL-6 (~7-fold) and IL6R (~17-fold). In addition, LPS-induced p38 MAPK and JNK MAPK activation was significantly increased in MCF-10ACE. Furthermore, CAV-1 co-localized with TLR4 and appeared a negative correlation trend. Conclusion: CAV-1 gene silencing promotes MAPK activation via TLR4 signaling in human mammary epithelial cells response to LPS.
Keywords
CAV-1; LPS-MAPK pathway; TLR4; mastitis; breast cancer;
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