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The Effects of Hyaluronic Acid-Carboxymethylcellulose Membrane (GUARDIX-$MB^{(R)}$) Barriers on Prevention of Post-operation Peritoneal Adhesions in Dogs  

Lee, Sang-Mook (College of Veterinary Medicine, Kyungpook National University)
Jang, Hwan-Soo (Department of Pharmacology, School of Medicine, Kyungpook National University)
Bae, Jae-Sung (Department of Physiology, School of Medicine, Kyungpook National University)
Kim, Jung-Eun (College of Veterinary Medicine, Kyungpook National University)
Jang, Kwang-Ho (College of Veterinary Medicine, Kyungpook National University)
Publication Information
Journal of Veterinary Clinics / v.25, no.6, 2008 , pp. 494-500 More about this Journal
Abstract
The aim of this study was to determine the effectiveness of hyaluronic acid-carboxymethylcellulose membrane (GUARDIX-$MB^{(R)}$) barriers on prevention against post-operative peritoneal adhesions. In this study, fourteen mongrel dogs were divided into two experimental groups: 0.1 % hyaluronic acid (0.1HA) group and hyaluronic acidcarboxymethylcellulose membrane (HA-CMC) group. In order to induce adhesions, the anti-mesenteric serosa of the ileum was exteriorized and then abraded in a standard manner by scraping with a scalpel blade to create homogenous petechial hemorrhagic surface over a $1\;{\times}\;1cm$ area. Solution of 0.1HA were simply coated over the abraded tissues, $1.5\;{\times}\;1.5cm$ HA-CMC membrane was placed over the abraded tissues, allowed to spread across the intra-abdominal organs before closure of the abdomen. On day 1 before and day 1, 4, 7, 14, and 21 after operation, venous blood specimens were collected for measurement of fibrinogen and total WBC. The adhesions were blindly assessed 3 weeks later by using a computerized tensiometer. The fibrinogen and total WBC values of two groups showed no statistical significances. The mean tensile strength (gram force, gf) of formed adhesions day 21 after surgery was $88.1\;{\pm}\;55.70gf$ in the 0.1 % HA group and $24.8\;{\pm}\;22.69gf$ in the HA-CMC group. The tensile strength values of adhesion separation HA-CMC membrane group was significantly lower than the 0.1HA group (p<0.05). Therefore, we suggest that HACMC membrane reduce peritoneal adhesions may be applicable to preventing post-operative intraperitoneal adhesions in dogs.
Keywords
hyaluronic acid-carboxymethylcellulose membrane; peritoneal adhesions; tensile strength; dogs;
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1 Abraham N. Morse. Robert A. Hammer. Jeffrey L. Cornella. Joseph C. Loftus. Validation of a Mouse Adhesion Reduction Model Using Seprafilm$^\circledR$. Journal of Gynecologic Surgery 2005; 21: 147-153   DOI   ScienceOn
2 Arnold PB, Green CW, Foresman PA, Rodeheaver GT. Evaluation of resorbable barriers for preventing surgical adhesions. Fertil Steril 2000; 73: 157-161   DOI   ScienceOn
3 Ara C, Kirimlioglu H, Karabulut AB, Coban S, Hascalik S, Celik O, Yilmaz S, Kirimlioglu V. Protective effect of melatonin against oxidative stress on adhesion formation in the rat cecum and uterine horn model. Life Sci. 2005;77:1341- 1350   DOI   ScienceOn
4 Berkkanoglu M, Zhang L, Ulukus M, Cakmak H, Kayisli UA, Kursun S, Arici A. Inhibition of chemokines prevents intraperitoneal adhesions in mice. Hum Reprod 2005; 20: 3047-3052   DOI   ScienceOn
5 Cohen Z, Senagore AJ, Dayton MT, Koruda MJ, Beck DE, Wolff BG, Fleshner PR, Thirlby RC, Ludwig KA, Larach SW, Weiss EG, Bauer JJ, Holmdahl L. Prevention of postoperative abdominal adhesions by a novel, glycerol/ sodium hyaluronate/carboxymethylcellulose-based bioresorbable membrane: a prospective, randomized, evaluator-blinded multicenter study. Dis Colon Rectum 2005; 48: 1130-1139   DOI
6 Ferland R, Mulani D, Campbell PK. Evaluation of a sprayable polyethylene glycol adhesion barrier in a porcine efficacy model. Hum Reprod 2001; 16: 2718-2723   DOI   ScienceOn
7 Jang KH, Kwon YS, Kim JE, Kwon EJ, Oh TH, Lee KW, Jang IH. Effect of carboxymethyl chitosan on postoperative intraperitoneal adhesion formation in the rat. Korean J Vet Res 2000; 40: 635-643
8 Ozel H, Avsar FM, Topaloglu S, Sahin M. Induction and assessment methods used in experimental adhesion studies. Wound Repair Regen 2005; 13: 358-364   DOI   ScienceOn
9 Reijnen MM, Bleichrodt RP, van Goor H. Pathophysiology of intra-abdominal adhesion and abscess formation, and the effect of hyaluronan. Br J Surg 2003; 90: 533-541   DOI   ScienceOn
10 Seeger JM, Kaelin LD, Staples EM, Yaacobi Y, Bailey JC, Normann S, Burns JW, Goldberg EP. Prevention of postoperative pericardial adhesions using tissue-protective solutions. J Surg Res 1997; 68: 63-66   DOI   ScienceOn
11 Sulaiman H, Dawson L, Laurent GJ, Bellingan GJ, Herrick SE. Role of plasminogen activators in peritoneal adhesion formation. Biochem Soc Trans 2002; 30: 126-131   DOI
12 Tarhan OR, Barut I, Sutcu R, Akdeniz Y, Akturk O. Pentoxifylline, a methyl xanthine derivative, reduces peritoneal adhesions and increases peritoneal fibrinolysis in rats. Tohoku J Exp Med 2006; 209: 249-255   DOI   ScienceOn
13 Kayaoglu HA, Ozkan N, Hazinedaroglu SM, Ersoy OF, Koseoglu RD. An assessment of the effects of two types of bioresorbable barriers to prevent postoperative intraabdominal adhesions in rats. Surg Today 2005; 35: 946-950   DOI
14 LeBlanc KA, Stout RW, Kearney MT, Paulson DB. Comparison of adhesion formation associated with Pro-Tack (US Surgical) versus a new mesh fixation device, Salute (ONUX Medical). Surg Endosc 2003; 17: 1409-1417   DOI
15 Vrijland WW, Tseng LN, Eijkman HJ, Hop WC, Jakimowicz JJ, Leguit P, Sta, Swank DJ, Haverlag R, Bonjer HJ, Jeekel H. Fewer intraperitoneal adhesions with use of hyaluronic acid-carboxymethylcellulose membrane: a randomized clinical trial. Ann Surg 2002; 235: 193-199   DOI   ScienceOn
16 Kang YH, Cheong JT, Yeon SC. Prevention of uterine adhesion by sodium carboxymethylcellulose in dogs. Korean J Vet Clin Med 2000; 17: 381-387   과학기술학회마을
17 Kwon YS, Jang KH. Effects of carboxymethyl chitosan fabric and low molecular weight heparin on reducing adhesin formation in the rat. Korea Vet Res 2003; 43: 703-708
18 Jang YK, Kim HH, Lee HC, Yeon SC, Lee HJ. Reduction of postoperative adhesions by a synthetic solution of sodium carboxymethylcellulose and dextran or interceed following intestinal anastomosis in dogs. Korean J Vet Clin Med 2004; 21: 349-354   과학기술학회마을
19 Sawada T, Hasegawa K, Tsukada K, Kawakami S. Adhesion preventive effect of hyaluronic acid after intraperitoneal surgery in mice. Hum Reprod 1999; 14: 1470-1472   DOI
20 Cornum R, Bell J, Gresham V, Brinkley W, Beall D, MacPhee M. Intraoperative use of the absorbable fibrin adhesive bandage: long term effects. J Urol 1999; 162: 1817-1820   DOI   ScienceOn
21 Han TS, Lee JM, Yun YM, Kang 쇼, Shin TK, Kang YH, Kim NJ, Kim HS, Lee KK, Cheong JT. Sodium carboxymethylcellulose and hyaluronic acid on prevention of intra-abdominal adhesion in rats. Korean J Vet Clin Med 2003; 20: 323-327   과학기술학회마을
22 Tarhan OR, Eroglu A, Cetin R, Y Nce A, Bulbul M, Altuntas YR. Effects of seprafilm on peritoneal fibrinolytic system. ANZ J Surg 2005; 75: 690-692   DOI   ScienceOn
23 Jung DK, Cheong JT, Lee KK, Kim HS, Choi NJ, Yeon SC, Seo KM. The effect of sodium carboxymethylcellulose on prevention of post-operative pleural and pericardial adhesions in dogs. Korean J Vet Clin Med 2000; 17: 368- 375   과학기술학회마을
24 Cheong JT, Lee KK, Jang KH. Prevention of abdominal Re-adhesions by sodium carboxymethylcelluloes in dogs with abdominal adhesions. Korean J Vet Clin Med 1997; 14: 161-167
25 Schafer M, Krahenb hl L, Buchler MW. Comparison of adhesion formation in open and laparoscopic surgery. Dig Surg 1998; 15: 148-152   DOI   ScienceOn
26 Klein ES, Asculai SS, Ben-Ari GY. Effects of hyaluronic acid on fibroblast behavior in peritoneal injury. J Surg Res 1996; 61: 473-476   DOI   ScienceOn
27 Bristow RE, Montz FJ. Prevention of adhesion formation after radical oophorectomy using a sodium hyaluronatecarboxymethylcellulose (HA-CMC) barrier. Gynecol Oncol 2005; 99: 301-308   DOI   ScienceOn
28 Ozmen MM, Ozalp N, Zulfikaroglu B, Abbasoglu L, Kacar A, Seckin S, Koc M. Histoacryl blue versus sutured left colonic anastomosis: experimental study. ANZ J Surg 2004; 74: 1107-1110   DOI   ScienceOn
29 Yamaner S, Kalayci M, Barbaros U, Balik E, Bulut T. Does hyaluronic acid-carboxymethylcellulose (HA-CMC) membrane interfere with the healing of intestinal suture lines and abdominal incisions? Surg Innov 2005; 12: 37-41   DOI
30 Jang HY, Yoon HY, Kim JY, Han HJ, Lee BR, Won HJ, Jeong SW. Evaluation of sodium carboxymethylcellulose for prevention of adhesion in intestinal anastomosis in dogs. Korean J Vet Res 2005; 45: 417-421
31 Lee YW, Lee YM. Evaluation on effectiveness for preventing pest surgical adhesion of sodium hyaluronatesodium carboxymethylcelluose (HA/CMC) membrane in rat cecum/peritonium model. Membrane journal 2005; 15: 213- 223   과학기술학회마을
32 Becker JM, Stucchi AF. Intra-abdominal adhesion prevention: are we getting any closer? Ann Surg 2004; 240: 202-204   DOI   ScienceOn
33 Oh DS, Manning MM, Emmanuel J, Broyles SE, Stone HH. Repair of full-thickness defects in alimentary tract wall with patches of expanded polytetrafluoroethylene. Ann Surg 2002; 235: 708-711   DOI   ScienceOn
34 Mirastschijski U, Johannesson K, Jeppsson B, Agren MS. Effect of a matrix metalloproteinase activity and TNF-alpha converting enzyme inhibitor on intra-abdominal adhesions. Eur Surg Res 2005; 37: 68-75   DOI   ScienceOn
35 Vural B, Canturk NZ, Esen N, Solakoglu S, Canturk Z, Kirkali G, Sokmensuer C. The role of neutrophils in the formation of peritoneal adhesions. Hum Reprod 1999; 14: 49-54   DOI   ScienceOn
36 Tingstedt B, Nehez L, Axelsson J, Lindman B, Andersson R. Increasing anastomosis safety and preventing abdominal adhesion formation by the use of polypeptides in the rat. Int J Colorectal Dis 2006; 21: 566-572   DOI
37 Miyamoto K, Masuda K, Inoue N, Okuma M, Muehleman C, An HS. Anti-adhesion properties of a thrombin-based hemostatic gelatin in a canine laminectomy model: a biomechanical, biochemical, and histologic study. Spine 2006; 31: 91-97   DOI   ScienceOn
38 Wang XC, Gui CQ, Zheng QS. Combined therapy of allantoin, metronidazole, dexamethasone on the prevention of intra-abdominal adhesion in dogs and its quantitative analysis. World J Gastroenterol 2003; 9: 568-571   DOI
39 Fazio VW, Cohen Z, Fleshman JW, van Goor H, Bauer JJ, Wolff BG, Corman M, Beart RW Jr, Wexner SD, Becker JM, Monson JR, Kaufman HS, Beck DE, Bailey HR, Ludwig KA, Stamos MJ, Darzi A, Bleday R, Dorazio R, Madoff RD, Smith LE, Gearhart S, Lillemoe K, Gohl J. Reduction in adhesive small-bowel obstruction by Seprafilm adhesion barrier after intestinal resection. Dis Colon Rectum 2006; 49: 1-11   DOI
40 Frederik J. T. van Oosterom, Jan J. B. van Lanschot, Johannes Oosting, Huug Obertop Hyaluronic acid/carboxymethylcellulose membrane surrounding an intraperitoneal or subcutaneous jejunojejunostomy in rats. Eur J Surg 2000; 166: 654-658   DOI
41 Bae JS, Jang KH, Kwon YS, Jang HS, Kim JE, Park SI, Lim JH, Li WX and Lee SJ. The effect of hyaluronic acid and vitamin E combination on preventing postoperative intraperitoneal adhesion formation in dogs. Korean J Vet Clin 2003; 20: 42-48
42 Hellebrekers BW, Emeis JJ, Kooistra T, Trimbos JB, Moore NR, Zwinderman KH, Trimbos-Kemper TC. A role for the fibrinolytic system in postsurgical adhesion formation. Fertil Steril 2005; 83: 122-129   DOI   ScienceOn
43 Jeremy Thompson. Pathogenesis and Prevention of Adhesion Formation. Digestive Surgery 1998; 15: 153-157   DOI   ScienceOn
44 Reijnen MM, Skrabut EM, Postma VA, Burns JW, van Goor H. Polyanionic polysaccharides reduce intra-abdominal adhesion and abscess formation in a rat peritonitis model. J Surg Res 2001; 101: 248-253   DOI   ScienceOn