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http://dx.doi.org/10.15616/BSL.2017.23.2.128

Effects of Streptozotocin, Bisphenol A and Diethylstilbestrol on Production of Reactive Oxygen Species and Lipid Peroxidation in the Boar Sperm  

Lee, A-Sung (College of Animal Life Sciences, Kangwon National University)
Lee, Sang-Hee (Institute of Animal Resources, Kangwon National University)
Lee, Seunghyung (College of Animal Life Sciences, Kangwon National University)
Yang, Boo-Keun (College of Animal Life Sciences, Kangwon National University)
Publication Information
Biomedical Science Letters / v.23, no.2, 2017 , pp. 128-132 More about this Journal
Abstract
Streptozotocin (STZ), bisphenol A (BPA), and diethylstilbestrol (DES) are known as endocrine disruptors, occurs oxidative stress in animal cells. Generally, oxidative stress induces reactive oxygen species (ROS) and lipid peroxidation of sperm and lead to decreased viability and fertility in pigs. Therefore, we investigated the influence of STZ, BPA and DES on ROS production and lipid peroxidation on boar sperm. Collected sperm were incubated with semen extender containing $10{\mu}M\;STZ$, $10{\mu}M\;BPA$ and $20{\mu}M\;DES$ for 3, 6 and 9 hours. Intracellular ROS and lipid peroxidation of sperm were analyzed by 2', 7'-dichlorofluorescein diacetate and malondialdehyde methods. The results show that, intracellular ROS was not significantly different among the all treatments, but lipid peroxidation was significantly increased in STZ group at 3 hour after incubation with boar sperm (P<0.05). These results suggest that STZ stimulates lipid peroxidation more than ROS production and may exert a negative effect on sperm fertility.
Keywords
Streptozotocin; Bisphenol A; Diethylstilbestrol; Reactive oxygen species; Lipid eroxidation; Sperm;
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