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http://dx.doi.org/10.4062/biomolther.2008.16.4.385

Anti-inflammatory Effects of the Methanol Extract of Polytrichum Commune via NF-κB Inactivation in RAW 264.7 Macrophage Cells  

Cho, Woong (Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University)
Park, Seung-Jae (Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University)
Shin, Ji-Sun (Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University)
Noh, Young-Su (Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University)
Cho, Eu-Jin (Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University)
Nam, Jung-Hwan (Highland Agricultural Institute, Rural Development Adminstration)
Lee, Kyung-Tae (Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University)
Publication Information
Biomolecules & Therapeutics / v.16, no.4, 2008 , pp. 385-393 More about this Journal
Abstract
As an attempt to search for bioactive natural products exerting anti-inflammatory activity, we evaluated the effects of the methanol extract of Polytrichum commune Hedw (PCM) (Polytrichaceae) on lipopolysaccharide (LPS)-induced nitric oxide (NO), prostaglandin $E_2$ ($PGE_2$) and pro-inflammatory cytokines release in murine macrophage cell line RAW 264.7. PCM potently inhibits the production of NO, $PGE_2$, tumor necrosis factor (TNF)-$\alpha$ and interleukin (IL)-6. Consistent with these results, PCM also concentration-dependently inhibited LPS-induced inducible NO synthase (iNOS) and cyclooxygase (COX)-2 at the protein levels, and iNOS, COX-2, TNF-$\alpha$ and IL-6 at the mRNA levels without an appreciable cytotoxic effect on RAW 264.7 macrophag cells. Furthermore, PCM inhibited LPS-induced nuclear factor-kappa B (NF-$\kappa$B) activation as determined by NF-$\kappa$B reporter gene assay, and this inhibition was associated with a decrease in the nuclear translocation of p65 and p50 NF-$\kappa$B. Taken together, these results suggest that PCM may play an anti-inflammatory role in LPS-stimulated RAW 264.7 macrophages through the inhibitory regulation of iNOS, COX-2, TNF-$\alpha$ and IL-6 via NF-$\kappa$B inactivation.
Keywords
Polytrichum commune; Inducible nitric oxide synthase (iNOS); Cyclooxygenase-2 (COX-2); Nuclear factor- kappa B (NF-$\kappa$B); Anti-inflammation;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 8  (Related Records In Web of Science)
Times Cited By SCOPUS : 6
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1 Vane, J.R. and Botting R. M. (2003). The mechanism of action of aspirin. Thromb Res. 110, 255-258   DOI   ScienceOn
2 Vane, J. R., Mitchell, J. A., Appleton, I., Tomlinson, A., Bishop-Bailey, D., Croxtall, J. and Willoughby, D. A. (1994). Inducible isoforms of cyclooxygenase and nitric-oxide synthase in inflammation. Proc. Natl. Acad. Sci. USA. 91, 2046-2050   DOI
3 Yun, H. Y., Dawson, T. T. and Dawson T. M. (1996). Neurobiology of nitric oxide. Crit. Rev. Neurobiol. 10, 291-316   DOI   ScienceOn
4 Prescott, S. M. and Fitzpatrick, F. A. (2000). Cyclooxygenase-2 and carcinogenesis. Biochim. Biophys. Acta. 1470, M69-M78
5 Doyle, S. L. and O'Neill, L. A. (2006). Toll-like receptors: from the discovery of NFkappaB to new insights into transcriptional regulations in innate immunity. Biochem. Pharmacol. 72, 1102-1113   DOI   ScienceOn
6 Rankin, J. A. (2004). Biological mediators of acute inflammation. AACN. Clin. Issues. 15, 3-17   DOI   ScienceOn
7 Smith, W., DeWitt, D. and Garavito, R. (2000). Cyclooxygenases: structural, cellular, and molecular biology. Annu. Rev. Biochem. 69, 145–182   DOI   ScienceOn
8 Aggarwal, B. B. and Natarajan, K. (1996). Tumor necrosis factors: developments during the last decade. Eur. Cytokine. Netw. 7, 93-124
9 Burg, N. D. and Pillinger, M. H. (2001). The neutrophil: function and regulation in innate and humoral immunity. Clin. Immunol. 99, W7-17   DOI   ScienceOn
10 Chen, C. Y., Peng, W.H., Tsai, K. D., and Hsu, S. L. (2007). Luteolin suppresses inflammation-associated gene expression by blocking NF-kappaB and AP-1 activation pathway in mouse alveolar macrophages. Life Sci. 81, 1602-14   DOI   ScienceOn
11 Fang, S. H., Rao, Y. K. and Tzeng, Y. M. (2008). Anti-oxidant and inflammatory mediator's growth inhibitory effects of compounds isolated from Phyllanthus urinaria. J Ethnopharmacol. 116, 333-340   DOI   ScienceOn
12 Guo, G. J. (2001). Directional migration of leukocytes: their pathological roles in inflammation and strategies for development of anti-inflammatory therapies. Cell. Res. 11, 85-88   DOI   ScienceOn
13 Hinz, B. and Brune, K. (2002). Cyclooxygenase-2-10 years later. J. Pharmacol. Exp. Ther. 300, 367-375   DOI   ScienceOn
14 Iontcheva, I., Amar, S., Zawawi, K.H., Kantarci, A. and Van Dyke, T.E. (2004). Role for moesin in lipopolysaccharide-stimulated signal transduction. Infect. Immun. 72, 2312-2320   DOI
15 Huerre, M. R. and Gounon, P. (1996). Inflammation: pattern and new concepts. Res. Immunol. 147, 417-434   DOI   ScienceOn
16 Nathan, C. (2002). Points of control in inflammation. Nature 420, 846-852   DOI   ScienceOn
17 Ivanova, V., Kolarova, M., Aleksieva, K., Dornberger, K. J., Haertl, A., Moellmann, U., Dahse, H. M. and Chipev N. (2007). Sanionins: anti-inflammatory and antibacterial agents with weak cytotoxicity from the Antarctic moss Sanionia georgico-uncinata. Prep. Biochem. Biotechnol. 37, 343-352   DOI   ScienceOn
18 Kang, Y. J., Mbonye, U. R., DeLong, C. J., Wada, M. and Smith, W. L. (2007). Regulation of intracellular cyclooxygenase levels by gene transcription and protein degradation. Prog. Lipid. Res. 46, 108-125   DOI   ScienceOn
19 Kim, K. S., Lee, S. H., Kang, K. H. and Kim, B. K. (2005). Flavonoid galactosides from Artemisia apiacea. Nat. Prod. Sci. 11, 10-11
20 Nimptsch, J. and Pflugmacher, S. (2008). Decomposing leaf litter: the effect of allochthonous degradation products on the antioxidant fitness and photosynthesis of Vesicularia dubyana. Ecotoxicol. Environ. Saf. 69, 541-545   DOI   ScienceOn
21 Park, M. H., Song, H. S., Kim, K. H., Son, D. J., Lee, S. H., Yoon, D. Y., Kim, Y., Park, I. Y., Song, S., Hwang, B. Y., Jung, J. K. and Hong, J. T. (2005). Cobrotoxin inhibits NF-kappa B activation and target gene expression through reaction with NF-kappa B signal molecules. Biochemistry 44, 8326-8236   DOI   ScienceOn
22 Kim, J. B., Han, A. R., Park, E. Y., Kim, J. Y., Cho, W., Lee, J., Seo, E. K. and Lee, K.T. (2007). Inhibition of LPS-induced iNOS, COX-2 and cytokines expression by poncirin through the NF-kappaB inactivation in RAW 264.7 macrophage cells. Biol. Pharm. Bull. 30, 2345-2351   DOI   ScienceOn
23 Park, J. C., Park, J. G., Hur, J. S., Choi, M. R., Yoo, E. J., Kim, S. H., Son, J. C. and Kim, M. S. (2004). Inhibitory effects of methanol extract, phenolic acids and favonoids from the leaves of Eucalyptus darylmpleana against 1,1-diphenyl-2-picrylhydrazyl radical. Nat. Prod. Sci. 10, 244-247
24 Feldmann, M., Brennan, F. M., Chantry, D., Haworth, C., Turner, M., Katsikis, P., Londei, M., Abney, E., Buchan, G., Barrett, K., Corcoran, A., Kissonerghis, M., Zheng, R., Gruberck-Loebenstein, B., Barkley, D., Chu, C. Q., Field, M. and Maini R.N. (1991). Cytokine assays: role in evaluation of the pathogenesis of autoimmunity. Immunol. Rev. 119, 105-123   DOI
25 Gordon, S. (2007). The macrophage: past, present and future. Eur J Immunol. 37 Suppl 1, S9-17   DOI   ScienceOn
26 Krasnow, S. W., Zhang, L. Q., Leung, K. Y., Osborn, L., Kunkel, S. and Nabel, G. J. (1991). Tumor necrosis factor-alpha, inter leukin 1, and phorbol myristate acetate are independent activators of NF-kappa B which differentially activate T cells. Cytokine 3, 372-379   DOI   ScienceOn
27 Lee, M. S., Lim, S. C. and Park, H. J. (1994). Phthalate Ester and Flavonoids Isolated From Leaves of Erythronium japonicum. Kor. J. Medicinal Crop Sci. 2, 67-72   과학기술학회마을
28 Dinarello, C. A. (2004). Infection, fever, and exogenous and endogenous pyrogens: some concepts have changed. J. Endotoxin. Res. 10, 201-222   DOI
29 Esser, R., Berry, C., Du, Z., Dawson, J., Fox, A., Fujimoto, R. A., Haston, W., Kimble, E. F., Koehler, J., Peppard, J., Quadros, E., Quintavalla, J., Toscano, K., Urban, L., van Duzer, J., Zhang, X., Zhou, S. and Marshall, P. J. (2005). Preclinical pharmacology of lumiracoxib: a novel selective inhibitor of cyclooxygenase-2. Br. J. Pharmacol. 144, 538-550   DOI   ScienceOn
30 Moncada, S., Palmer, R. M. and Higgs, E. A. (1991). Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol. Rev. 43, 109-142
31 Inaba, H., Tagashira, M., Honma, D., Kanda, T., Kou, Y., Ohtake, Y. and Amano, A. (2008). Identification of hop polyphenolic components which inhibit prostaglandin E2 production by gingival epithelial cells stimulated with periodontal pathogen. Biol Pharm Bull. 31, 527-530   DOI   ScienceOn
32 Wang, C., Deng, L., Hong, M., Akkaraju, G. R., Inoue, J. and Chen, Z. J. (2001). TAK1 is a ubiquitin-dependent kinase of MKK and IKK. Nature 412, 346-351   DOI   ScienceOn
33 Hasan, A., Ahmed, L., Jay, M. and Voirin, V. (1995). Flavonoid glycosides and anthraquinone from Rumex chulepensis. Phytochemistry 39, 1211-1213   DOI   ScienceOn