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Effects of the Constituents of Paeonia lactiflora Root on Arachidonate and NO Metabolism  

Choi, Yong-Hwan (College of Pharmacy, Kangwon National University)
Gu, Lianyu (Natural Products Research Institute, College of Pharmacy, Seoul National University)
Kim, Yeong-Shik (Natural Products Research Institute, College of Pharmacy, Seoul National University)
Kang, Sam-Sik (Natural Products Research Institute, College of Pharmacy, Seoul National University)
Kim, Ju-Sun (Natural Products Research Institute, College of Pharmacy, Seoul National University)
Yean, Min-Hye (Natural Products Research Institute, College of Pharmacy, Seoul National University)
Kim, Hyun-Pyo (College of Pharmacy, Kangwon National University)
Publication Information
Biomolecules & Therapeutics / v.14, no.4, 2006 , pp. 216-219 More about this Journal
Abstract
In order to establish the anti-inflammatory cellular mechanism of the paeony root(Paeonia lactiflora, Pall, Paeoniaceae), the constituents including paeoniflorin, albiflorin, (+)-catechin, paeonol, benzoic acid and methyl gallate were evaluated for their effects on arachidonate and NO metabolism. Among the compounds tested, only paeonol weakly inhibited cyclooxygenase-2-mediated $PGE_2$ production from LPS-treated RAW 264.7 cells. (+)-Catechin and methyl gallate weakly inhibited inducible nitric oxide synthase-mediated NO production from the same cell line. In particular, methyl gallate significantly inhibited 5-lipoxygenase from RBL-l cells with an $IC_{50}$ of 8.4 ${\mu}M$. These results suggest that the inhibition of these components on arachidonate and NO metabolism may contribute at least in part to anti-inflammatory mechanism of the paeony root.
Keywords
Paeonia lactiflora; cyclooxygenase; nitric oxide synthase; lipoxygenase; methyl gallate;
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