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Induction of Nitric Oxide Production by Bafilomycin A1 in Mouse Leukemic Monocyte Cell Line  

Hong, Jang-Ja (Laboratory of Pathophysiology, College of Pharmacy, Sookmyung Women's University)
Nakano, Yasuhiro (Laboratory of Pathophysiology, College of Pharmacy, Sookmyung Women's University)
Ohuchi, Kazuo (Laboratory of Biochemistry, Faculty of Pharmacy, Yasuda Women's University)
Kang, Young-Sook (Laboratory of Pathophysiology, College of Pharmacy, Sookmyung Women's University)
Publication Information
Biomolecules & Therapeutics / v.14, no.3, 2006 , pp. 143-147 More about this Journal
Abstract
In the mouse leukemic monocyte cell line RAW 264.7, the vacuolar-type $(H^+)$-ATPase (V-ATPase) inhibitor bafilomycin $A_1$ at 10 and 100 nM decreased cell growth and survival as determined by 3-(4,5-dimethyl(thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in a concentration-dependent manner. At such concentrations, bafilomycin $A_1$ induced nitric oxide (NO) production through the expression of inducible nitric oxide synthase (iNOS). The bafilomycin $A_1$-induced NO production was inhibited by the NOS inhibitor $N^G$-monomethyl-L-arginine acetate (L-NMMA). Our findings suggest that the V-ATPase inhibitor bafilomycin $A_1$ induces NO production through the expression of iNOS protein.
Keywords
RAW 264.7 cells; Bafilomycin $A_1$; Nitric oxide; V-ATPase inhibitor;
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