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Neutralizing Effects of Antiserum by Repeated Subcutaneous Administration of Recombinant Human Growth Hormone (rhGH)  

Song, Yeon-Jung (Institute of Bioscience ＆ Biotechnology, Daewoong Pharmaceutical Co.)
Park, Shin-Hye (Institute of Bioscience ＆ Biotechnology, Daewoong Pharmaceutical Co.)
Park, Seung-Kook (Institute of Bioscience ＆ Biotechnology, Daewoong Pharmaceutical Co.)
Yeon, Je-Deuk (Institute of Bioscience ＆ Biotechnology, Daewoong Pharmaceutical Co.)
Publication Information
Biomolecules & Therapeutics / v.10, no.3, 2002 , pp. 175-179 More about this Journal
Abstract
Human growth hormone (hGH) forms antibody by repeated administration. The present study investigated to confirm formation of antibody by repeated subcutaneous administration of hGH for two months in rats and dogs. In this result, hGH-injected sera were significantly higher than control sera by 1:1,000,000 of dilution factor. After antibody formed sera (anti-hGH sera) and control sera were added to 30 $\mu\textrm{g}$/ml hGR, the complex incubated for overnight at $30^{\circ}C$. Anti-hGH sera decreased hGH contents about 90% compared to control sera. Also, body weight gain conducted decreased about 67% compared to control sera in hypophysectomised rat. Inconclusion, repeated administration of hGH formed antibody because hGH was foreign protein to rats and dogs. And formed antibody of hGH was blocked and decreased many efficacy of hGH, the antibody was proved to be neutralizing antibody. Thus, because neutralizing antibodies were decreased pharmacological effects of hGH, administration more than two months were no significance.
Keywords
Human growth hormone (hGH); antibody; neutralizing activity; hypophysectomised rat; body weight gain;
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