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http://dx.doi.org/10.7783/KJMCS.2013.21.6.466

Effects of Supercritical Fluid Extract, Shikonin and Acetylshikonin from Lithospermum erythrorhizon on Chondrocytes and MIA-Induced Osteoarthritis in Rats  

Kim, Geum Soog (Department of Herbal Crop Research, NIHHS, RDA)
Kim, Hwa Jin (YD Life Science Research Institute, YD Life Science Co. Ltd.)
Lee, Dae Young (Department of Herbal Crop Research, NIHHS, RDA)
Choi, Seung Min (YD Life Science Research Institute, YD Life Science Co. Ltd.)
Lee, Seung Eun (Department of Herbal Crop Research, NIHHS, RDA)
Noh, Hyung Jun (Department of Herbal Crop Research, NIHHS, RDA)
Choi, Jong Gil (YD Life Science Research Institute, YD Life Science Co. Ltd.)
Choi, Soo Im (YD Life Science Research Institute, YD Life Science Co. Ltd.)
Publication Information
Korean Journal of Medicinal Crop Science / v.21, no.6, 2013 , pp. 466-473 More about this Journal
Abstract
This study investigates the effect of supercritical fluid extract (CMPB803-C) of Lithospermum erythrorhizon, shikonin and acetylshikonin isolated from Lithospermum erythrorhizon on IL-$1{\beta}$-induced chondrocytes and monosodium iodoacetate (MIA)-induced osteoarthritis in rat. Shikonin ($50{\mu}m$) and acetylshikonin ($3{\mu}M$) treatment reduced significantly the mRNA expression and enzyme activity of matrix metalloproteinase (MMP)-1, -3 and -13 in IL-$1{\beta}$-induced SW1353 chondrosarcoma cells. The chondro-protective effects of CMPB803-C and acetylshikonin were than analyzed in a rat OA model using a single intra-articular injection of MIA (1mg) in the right knee joint. CMPB803-C (200mg/kg) or acetylshikonin (5mg/kg) was orally administered daily for two weeks starting after 1 week of MIA injection. In the histological observation, CMPB803-C and acetylshikonin clearly improved OA lesions being comparable to or better that control group. Our results demonstrated that CMPB803-C and acetylshikonin as active compound of Lithospermum erythrorhizon have a strong chondro-protective effect in OA rats, which likely attributes to its anti-inflammatory activity and inhibition of MMPs production.
Keywords
Lithospermum erythrorhizon; Shikonin; Acetylshikonin; Osteoarthritis; MIA; MMP;
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Times Cited By KSCI : 5  (Citation Analysis)
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