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Detection Method for Unapproved Genetically Modified Rose Plants in Korea Using Duplex Polymerase Chain Reaction  

Kim, Jae-Hwan (Institute of Life Science & Resources, Kyung Hee University)
Park, Young-Doo (Institute of Life Science & Resources, Kyung Hee University)
Kim, Hae-Yeong (Institute of Life Science & Resources, Kyung Hee University)
Publication Information
Horticultural Science & Technology / v.28, no.4, 2010 , pp. 672-677 More about this Journal
Abstract
A duplex PCR method was developed to detect a transformation vector pSPB130 used in the development of a genetically modified (GM) rose plant. To detect a GM rose plant, the anthocyanin synthase ($ANS$) was used as an endogenous reference gene of rose in PCR detection. The primer pair RHANS-KF/KR producing 107 bp amplicon was used to amplify the $ANS$ gene and no amplified product was observed in any of the 9 different plants used as a template. The primer pair GMRH-KF/KR was designed to amplify the junction sequence between 35S promoter and flavonoid 3',5'-hydroxylase ($F3^{\prime}5^{\prime}H$) gene in pSPB130. The detection limit of the duplex PCR method is approximately 0.5%. This result indicates that this duplex PCR method could be useful for monitoring unauthorized GM rose in Korea.
Keywords
flavonoid 3',5'-hydroxylase; pSPB130; primer; transformation vector;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
Times Cited By SCOPUS : 0
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