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http://dx.doi.org/10.11625/KJOA.2021.29.2.241

Effects of Additional Levels of Phyllostachys bambusoides on Ruminal Fermentation Characteristics and Methane Emission in in vitro  

Jo, Seong-Uk (경상국립대학교 응용생명과학부(BK21))
Lee, Shin-Ja (경상국립대학교 농업생명과학연구원&중점연구소)
Lee, Ye-Jun (경상국립대학교 응용생명과학부(BK21))
Kim, Hyun-Sang (경상국립대학교 응용생명과학부(BK21))
Eom, Jun-Sik (경상국립대학교 응용생명과학부(BK21))
Choi, You-Young (경상국립대학교 응용생명과학부(BK21))
Bae, Eun-Ji (국립산림과학원 산림바이오소재연구소)
Lee, Sung-Sill (경상국립대학교 응용생명과학부(BK21), 농업생명과학연구소 & 중점연구소)
Publication Information
Korean Journal of Organic Agriculture / v.29, no.2, 2021 , pp. 241-256 More about this Journal
Abstract
The current study was to evaluate the antioxidant activity of Phyllostachys bambusoides (PHB) as a feed additives and investigate whether its antioxidant activity could be helpful for increasing rumen fermentation characteristics and methane reduction. The antioxidant activity results showed that total polyphenols and flavonoids contents were 43.54 ± 8.68 mg CE/g and 17.13 ± 0.45 mg QE/g, respectively, and the IC50 values for 1,1-diphenyl-2-prcrylhydrazyl (DPPH) and 2,2'-azino-bis (3- ethylbenzthiazoline-6- sulphonic acid) (ABTS) radical scavenging activity were 163.13 ± 19.25 ㎍/mL and 97.07 ± 4.46 ㎍/mL, respectively. Two heads of cannulated Hanwoo (450 ± 30 kg), consuming timothy hay and a commercial concentrate (60:40, w/w) twice daily (at 09:00 and 17:30) at 2% of body weight, with free access to water and a mineral block, were used as rumen fluid donors. An in vitro incubation experiment was performed after 6, 12, 24, 48, and 72 hr with PHB added at concentration of 2, 4, and 6% of timothy hay basis. Total gas emission decreased as the amount of PHB addition increased at 6 and 24 hr of incubation. However, PHB addition did not affect total volatile fatty acid production, and methane and carbon dioxide emission also decreased as the amount of addition increased at 48 hr of incubation. Therefore, PHB was expected to be used as methane reducing additives in the ruminants.
Keywords
antioxidant activity; bamboo leaf; ruminant; methane emission; volatile fatty acid;
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