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http://dx.doi.org/10.7585/kjps.2014.18.4.396

Isolation and Characterization of Actinomycete Strain BK185 Possessing Antifungal Activity against Ginseng Root Rot Pathogens  

Kim, Byung-Yong (Agricultural Microbiology Division, National Academy of Agricultural Science)
Bae, Mun-Hyung (National Products Research Institute, College of Pharmacy, Seoul National University)
Ahn, Jae-Hyung (Agricultural Microbiology Division, National Academy of Agricultural Science)
Weon, Hang-Yeon (Agricultural Microbiology Division, National Academy of Agricultural Science)
Kim, Sung-Il (Institute of Ginseng and Medicinal Plants Research, Gangwon Agricutlural Research and Extension Services)
Kim, Wan-Kyu (Agricultural Microbiology Division, National Academy of Agricultural Science)
Oh, Dong-Chan (National Products Research Institute, College of Pharmacy, Seoul National University)
Song, Jaekyeong (Agricultural Microbiology Division, National Academy of Agricultural Science)
Publication Information
The Korean Journal of Pesticide Science / v.18, no.4, 2014 , pp. 396-403 More about this Journal
Abstract
Ginseng (Panax ginseng C. A. Meyer) is an economically valuable pharmaceutical crop in Korea. In order to find promising biocontrol agents for soil-borne fungal pathogens which infect ginseng roots, we have isolated actinomycete, BK185 from soil. The isolate was investigated for the antifungal activity against to ginseng rot pathogens prior to testing genetic and chemical properties. The strain was identified as Streptomyces sp. using phylogenetic analysis based on 16S rRNA gene sequence. The most closely related species was S. sporoclivatus and S. geldanamycininus with high similarities (>99%). The isolate, BK185 showed positive reaction for PCR detection targeting biosynthetic gene clusters of PKS (Type-I polyketide synthase) and NRPS (Non-ribosomal polypeptide synthetase) genes. Major metabolite from the BK185 was analyzed by The LC/MS and identified to geldamycin, which was known to contained broad antibacterial, antifungal or anticancer activities. The results provide evidences that the strain, BK185 can be promising biocontrol agent for ginseng organic farming.
Keywords
ginseng rot; antifungal activity; Streptomyces; BK185; geldanamycin;
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Times Cited By KSCI : 5  (Citation Analysis)
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