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Analysis of Constituents in Sipjundaebo-tangs Fermented by Lactic Acid Bacteria  

Yang, Min-Cheol (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine)
Jeong, Sang-Won (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine)
Ma, Jin-Yeul (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine)
Publication Information
Microbiology and Biotechnology Letters / v.39, no.4, 2011 , pp. 350-356 More about this Journal
Abstract
The purpose of this study was to investigate changes in the contents of constituents in Sipjundaebo-tang (SJ) and its fermentations (FSJ) with 8 species of lactic acid bacteria. Eight strains of lactic acid bacteria, Lactobacillus casei KFRI129, L. plantarum KFRI 144, L. curvatus KFRI 166, L. hilgardii KFRI 229, L. delbruekil subsp. lactis KFRI 442, L. casei KFRI 692, L. gasseri KCTC 3163, and Bifidobacterium breve KFRI 744, were utilized for the fermentation of Sipjundaebo-tang. As a result we identified three constituents which increased and two constituents which decreased. The increased constituents were isolated by chromatographic techniques and then their structure elucidated using NMR and MS. The decreased constituents were confirmed by comparing standard compounds. These compounds were quantitatively analyzed using the HPLC/DAD system. The increased constituents were identified to be cinnamyl alcohol (1), liquiritigenin (3), and nodakenetin (5), while the decreased constituents were liquiritin (2) and nodakenin (4). Generally, liquiritin (2) and nodakenin (4) were noted as having decreased in fermented Sipjundaebo-tangs (FSJs), while cinnamyl alcohol (1), liquiritigenin (3), and nodakenetin (5) were seen to have increased. Sipjundaebo-tang fermented by L. plantarum KFRI144 exhibited the most remarkable changes in all of fermentations. The eight lactic acid bacteria all demonstrated differing decomposable rates on the five maker compounds in fermented Sipjundaebo-tang (FSJ).
Keywords
Sipjundaebo-tang; fermentation; cinnamyl alcohol; liquiritigenin; nodakenin; HPLC/DAD;
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1 Yang, M. J., Y. J. Kim, and T. S. Park. 2003. Effect of dietary supplementation of sypjeondaebotang or jahyulyanggeuntang on iron bioavailability in rats. The Korean Nutrition society. 36: 262-269.
2 You, Y. H., J. H. Koh, S. O. Chung, W. J. Jun, and K. M. Kim. 2009. Effects of fermented Ssanghwatang on swimming capacity in mice. Food. Sci. Biotechnol. 18: 275-277.
3 Lee, H. S. 2002. Inhibitory activity of Cinnamomum cassia bark-derived component against rat lens aldose reductase. J. Pharm. Pharm. Sci. 5: 226-230.
4 Lee, H. S., B. S. Kim, and M. K. Kim. 2002. Suppression effect of Cinnamomum cassia bark-derived component on nitric oxide synthase. J. Agric. Food. Chem. 50: 7700-7703.   DOI   ScienceOn
5 Lee, S. H., D. S. Shin, J. S. Kim, K. B. Oh, and S. S. Kang. 2003. Antibacterial coumarins from Angelica gigas roots. Arch. Pharm. Res. 26: 449-452.   DOI
6 Lee, S. Y., H. W. Kim, G. Y. Kim, C. H. Choi, Y. C. Yun, and H. W. Jung. 2009. Protective effects and anti-oxidative effects of sipjeon-daebo-tang and gami-sipjeon-daebo-tang in c6 glioma cell. Kor. J. Oriental Physiology & Pathology. 23: 1292-1298.
7 Mersereau, J. E., N. Levy, R. E. Staub, S. Baggett, T. Zogovic, S. Chow, A. R. William, T. Mary, C. Isaac, F. B. Leonard, and C. L. Dale. 2008. Liquiritigenin is a plantderived highly selective estrogen receptor beta agonist. Mol. Cell. Endocrinol. 283: 49-57.   DOI
8 Choi, J. H., J. N. Choi, S. Y. Lee, S. J. Lee, K. H. Kim, and Y. K. Kim. 2010. Inhibitory activity of diacylglycerol acyltransferase by glabrol isolated from the roots of licorice. Arch. Pharm. Res. 33: 237-242.   DOI
9 Jung, B. G., J. H. Ko, S. J. Cho, H. B. Koh, S. R. Yoon, D. U. Han, and B. J. Lee. 2010. Immune-enhancing effect of fermented Maesil (Prunus mume Siebold & Zucc.) with probiotics against Bordetella bronchiseptica in mice. J. Vet. Med. Sci. 72: 1195-1202.   DOI   ScienceOn
10 Kim, D. K., J. S. Eun, H. Jeon, and J. M. Song. 1998. Effect of sipjeon-daebo-tang on thymocytes of anti-tumor drugs administered mice. J. Herbology. 13: 129-135.
11 Kim, H. S. 1976. A study on the effect of SIM ZUN DAE BO TANG administered in rabbit with hepatic disfunction on the rate of recovery. Kor. J. Orient. Int. Med. 1: 78-84.
12 Shin, D. W. 2010. Hannyhak-GangLon II. p. 327. Shinilbooks Press, Seoul, Korea.
13 Kim, Y. W., R. J. Zhao, S. J. Park, J. R. Lee, I. J. Cho, C. H. Yang, S. J. Kim, and S. C. Kim. 2008. Anti-inflammatory effects of liquiritigenin as a consequence of the inhibition of NF-kappaB-dependent iNOS and proinflammatory cytokines production. Br. J. Pharmacol. 154: 165-173.   DOI   ScienceOn
14 Kwak, C. S., M. S. Lee, and S. C. Park. 2007. Higher antioxidant properties of Chungkookjang, a fermented soybean paste, may be due to increased aglycone and malonylglycoside isoflavone during fermentation. Nutr. Res. 27: 719- 727.   DOI   ScienceOn
15 Heo, J. 1993. Hangul Donguibogam. p. 817. Minjoong- Seogak, Seoul, Korea.
16 Zhang, S. P., Y. J. Zhou, Y. Liu, and Y. Q. Cai. 2009. Effect of liquiritigenin, a flavanone existed from Radix glycyrrhizae on pro-apoptotic in SMMC-7721 cells. Food. Chem. Toxicol. 47: 693-701.   DOI   ScienceOn
17 Okabe, Y., T. Shimazu, and H. Tanimoto. 2011. Higher bioavailability of isoflavones after a single ingestion of aglycone-rich fermented soybeans compared with glucosiderich non-fermented soybeans in Japanese postmenopausal women. J. Sci. Food. Agric. 91: 658-663.   DOI   ScienceOn
18 Park, J. H., H. J. Kim. and M. J. Lee. 2009. The role of gut microbiota in obesity and utilization of fermented herbal extracts. Journal of Society of Korean Medicine for Obesity Research. 12: 1-14.
19 Park, J. H., J. U. Jung, K. T. Kweon, M. J. Seo, E. K. Seo, Y. K. Park, and J. H. Lee. 2010. Nodakenin and decursin contents of fermented Angelicae Gigantis Radix by 4 species strain. J. Herbology. 25: 7-10.
20 Pouchert, C. and J. Behnke. 1993. The Aldrich Library of $^{13}C$ and $^{1}H$ FT NMR spectra edition 1 volume2. p 392. Aldrich Chemical Co., St. Louis, USA