Browse > Article

Monitoring of Antimicrobial Resistant Bacteria from Animal Farm Environments in Korea  

Kwon, Young-Il (Test and Research Center, Korea Consumer Protection Board)
Kim, Tae-Woon (Graduate School of Biotechnology, Kyung Hee University)
Kim, Hae-Yeong (Graduate School of Biotechnology, Kyung Hee University)
Chang, Yun-Hee (Department of Food and Nutrition, Myongji University)
Kwak, Hyo-Sun (Center for Food Safety Evaluation, Korea Food and Drug Administration)
Woo, Gun-Jo (Center for Food Safety Evaluation, Korea Food and Drug Administration)
Chung, Yun-Hee (Test and Research Center, Korea Consumer Protection Board)
Publication Information
Microbiology and Biotechnology Letters / v.35, no.1, 2007 , pp. 17-25 More about this Journal
Abstract
The kinds and quantity of antimicrobial agents used for cattle (animal industry) may be considerable, suggesting the possibility that pathogenic bacteria which cannot be extirpated by the existing antimicrobial agents could appear. Ten cattle, pig and chicken farms, respectively, were randomly selected from 5 provinces in Korea and the samples were collected from excrement, manure, underground water, farmers' hands and the neishboring environment. h total of 299 samples were examined and 197 of Escherichia coli, 13 of Campylobacter jejun/coli, 223 of Enterococcus faecium/faecalis and 42 of Staphylococcus aureus isolates were collected. All isolates were screened for antimicrobial resistance: 69.4% of E. coli (137/197 strains), 78.6% of S. aureus (33/42 strains), and 82.1% of E. faecium/faecalis (183/223 strains) were resistant to one antimicrobial agent and all of C. jejuni/coli Isolates showed the resistance to one antimicrobial agent. Meanwhile, the multiple resistance ratio for more than 4 lines of antimicrobial agent was 19.2% of E. coli (38/197 strains), 11.9% of S. aureus (5/42 strains), 15.4% of C. jejuni/coli (2/13 strains) and 6.2% of E. faecium/faecalis (14/223 strains). The antimicrobial resistance ratio of bacteria isolated from the cattle farm showed lower than that of bacteria isolated from the pig or chicken farm, which might be related to the quantify of antimicrobial agents consumed. And one strain of vancomycin resistant E..faecium (VREF) were isolated from the excrement of chicken and stream, respectively. Generally, the ratio of VREF collected in animal farm environments is lower than that of VREF collected in medical environment.
Keywords
Antimicrobial resistant bacteria; multi-drug resistant;
Citations & Related Records

Times Cited By SCOPUS : 4
연도 인용수 순위
1 Cloak, O. M. and P. M. Fratamico. 2002. A multiplex polymerase chain reaction for the differentiation of Campylobacter jujuni and Campylobacter coli from a swine processing facility and characterization of isolates by pulsed-field gel electrophoresis and antimicrobial resistance profiles. J. Food Prot. 65: 266-273   DOI   PUBMED
2 Delpassand E. S., M. V. Chari, C. E. Stager, J. D. Morrisett, J. I. Ford, and M. Romazi. 1995. Rapid identification of common human pathogens by high-resolution proton magnetic resonance spectroscopy. J. Clin. Microbiol. 33: 1258-1262   PUBMED
3 FDA. 2001. Antimicrobial Resistance A Growing Threat
4 Horn, K. G, C. A. Gedris, and K. M. Rodney. 1996. Selective isolation of vancomycin-resistant Enterococci. J. Clin. Microbiol. 34: 924-927   PUBMED
5 Institute of resistant bacteria. 2003. Susceptibility of bacteria for antimicrobials isolated in the year 2002. News, 11: 1-2
6 Kohner, P. C., R Patel, J. R. UbI, K. M. Garin, M. K. Hopkins, L. T. Wegener, and F. R. Cockerill. 1997. Comparison of agar dilution, broth microdilution, E-test, disk diffusion, and automated Vitek methods for testing susceptibilities of Enterococcus spp. to vancomycin. J. Clin. Microbiol. 35: 3258-3263   PUBMED
7 Ng S. P., C. O. Tsui, D. Roberts, P. Y. Chau, and M. H. Ng. 1996. Detection and serogroup differentiation of Salmonella spp. in food within 30 hours by enrichment-immunoassay with a T6 monoclonal antibody capture enzyme-linked immunosorbent assay. Appl. Environ. Microbiol. 62: 2294-2302   PUBMED
8 Pignato, S., A. M. Marino, M. C. Emanuele, V. Iannotta, S. Caracappa, and G. Giammanco. 1995. Evaluation of new culture media for rapid detection and isolation of Salmonella in foods. Appl. Environ. Microbiol. 61: 1996-1999   PUBMED
9 Song, J. H., J. W. Yang, J. H. Joung, S. J. Kang, and N. Y. Lee. 2000. Unique alterations in Penicillin-binding protein 2B of multidrug-resistant Streptococcus pneumoniae from Korea. Kor. J. Infect. Dis. 32: 108-114
10 Baele, M., P. Baele, M. Vaneechoutte, V. Storms, P. Butaye, L. A. Devriese, G Verschraegen, M. Gillis, and F. Haesebrouck. 2000. Application of tRNA intergenic spacer PCR for Identification of Enterococcus Species. J. Clin. Microbiol. 38: 4201-4207   PUBMED
11 Chung, Y. H., S. Y. Kim, and Y. H. Chang. 2003. Prevalence and antimicrobial susceptibility of Salmonella isolated from foods in Korea from 1993 to 2001. J. Food Prot. 66: 1154-1157   DOI   PUBMED
12 Dutka-Malen, S., S. Evers, and P. Courvalin. 1995. Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant Enterococci by PCR. J. Clin. Microbiol. 33: 24-27   PUBMED
13 Khan, S. A., M. S. Nawaz, A. A. Khan, R. S. Steele, and C. E. Cerniglia. 2000. Characterization of erythromycin-resistant methylase genes from multiple antimicrobial resistant Staphylococcus spp. isolated from milk samples of lactating cows. Am. J. Vet. Res. 61: 1128-1132   DOI   ScienceOn
14 Kim, J. S., Y. S. Lee, J. K. Lee, J. I. You, H. B. Kim,Y. H. Choi, and Y. J. Kim. 2000. The survey of vancomycin resistant Enterococcus isolated from clinic and animals. KNIH. 37: 40-52
15 National Committee for Clinical Laboratory Standards. 2001. Performance standards for antimicrobial disk susceptibility tests, 7th ed. Approved standard MA7. National Committee for Clinical Laboratory Standards. Wayne. PA
16 Pinto, B., R. Pierotti, G Canale, and D. Reali. 1999. Characterization of faecal streptococci as indicator of faecal pollution and distribution in the environment. Lett. Appl. Microbiol. 29: 258-263   DOI   ScienceOn
17 Bernard Dixon. 2000. Antimicrobials as growth promoters : risks and alternatives. ASM News. 66: 264-265
18 Jensen, M. W., W. C. Downs, J. D. Murray, T. R. Nicoll, S. D. Lefevre, and C. M. Meyers. 1987. Staphylococcosis of turkey. 1. portal of entry and tissue colonization. Avian Dis. 31: 64-69   DOI   ScienceOn
19 Boerlin, P., P. Kuhnert, D. Hussy, and M. Schaellibaum. 2003. Methods for identification of Staphylococcus aureus isolates in cases of bovine mastitis. J. Clin. Microbiol. 41: 767-771   DOI
20 Doorn, L-J. A. V. Haperen, A. Burnens, M. Huysmans, P. Vandamme, B. A. J. Giesendorf, M. J. Blaser, and W. G Quint. 1999. Rapid identification of thermotolerant Campylobacter jejuni, Campylobacter coli, Campylobacter lari, and Campylobacter upsaliensis from various geographic locations by a GrPase-based PCR-reverse hybridization assay. J. Clin. Microbiol. 37: 1790-1796   PUBMED
21 Hong, Y. M. E. Berrang, J. Liu, C. L. Hofacre, S. Sanchez, L. Wang, and J. J. Maurer. 2003. Rapid detection of Campylobacter coli, C. jejuni, and Salmonella enterica on poultry carcasses by using PCR-enzyme-linked immunosorbent assay. Appl. Environ. Microbiol. 69: 3492-3499   DOI
22 Rhodes, G., G. Huys, H. Swings, P. McGann, M. Hiney, P. Smith, and W. P. Roger. 2000. Distribution of oxytetracycline resistance plasmids between aeromonads in hospital and aquaculture environments: implication of Tnl in dissemination of the tetracycline resistance determinant Tet A. Appl. Environ. Microbiol. 66:3883-3890   DOI
23 Kuhnert P., J. Nicolet, and J. Frey. 1995. Rapid and accurate identification of Escherichia coli K-12 strains. Appl. Environ. Microbiol. 61: 4135-4139   PUBMED
24 Rice B. E., C. Lamichhane, S. W. Joseph, and D. M. Rollins. 1996. Development of a rapid and specific colony-lift immunoassay for detection and enumeration of Campylobacter jejuni, C. coli, and C. lari. Clin. Diagn. Lab. Immunol. 3: 669-677   PUBMED
25 Moschetti, G., G. Blaiotta, F. Villani, S. Coppola, and E. Parente. 2001. Comparison of statistical methods for identification of Streptococcus thermophilus, Enterococcus faecalis, and Enterococcus faecium from randomly amplified polymorphic DNA patterns. Appl. Environ. Microbiol. 67: 2156-2166   DOI   ScienceOn
26 Saha, S. K., G. L. Darmstadt, A. H. Baqui, M. Hanif, M. Ruhulamin, M. Santosham, T. Nagatake, and R. E. Black. 2001. Rapid Identification and antimicrobial susceptibility testing of Salmonella enterica Serovar Typhi isolated from blood: implications for therapy. J. Clin. Microbiol. 39: 3583-3585   DOI   ScienceOn
27 Holliday, M. G, M. Ford, J. D. Perry, and F. K. Goul. 1999. Rapid identification of Staphylococcus aureus by using fluorescent staphylocoagulase assays. J. Clin. Microbiol. 37: 1190-1192   PUBMED
28 Saenz, Y., M. Zarazaga, M. Lantero, M. J. Gastanares, F. Baquero, and C. Torres. 2000. antimicrobial resistance in Campylobacter strains isolated from animals, foods, and humans in Spain in 1997-1998. Antimicrob. Agents Chemother. 1: 267-271
29 Lee, K. Y. 2002, Monitoring of antimicrobial resistant bacteria in domestic. pp. 23-25. National Institute of Toxicological Research
30 Joseph S. W., J. R. Hayes, L. L. English, L. E. Carr, and D. D. Wagner. 2001. Implications of multiple antimicrobial-resistant enterococci associated with the poiltry environment. Food Addit. Contam. 18: 1118-1123   DOI
31 Wolfgang, W. 1998. Medical consequences of antimicrobial use in agriculture. Science. 13279: 996-997
32 Jung, S. C. (National Veterinary Research and Quarantine Service) 2003. Report on control of antimicrobial resistant bacteria in Korea. pp. 198-203. Construction of control system of antimicrobials for domestic animals. Korea Food and Drug Administration
33 Waage, A. S., T. Vardund, V. Lund, and G Kapperud. 1999. Detection of small numbers of Campylobacter jejuni and Campylobacter coli cells in environmental water, sewage, and food samples by a seminested PCR Assay. Appl. Environ. Microbiol. 65: 1636-1643   PUBMED
34 Chapin K. and M. Musgnug. 2003. Evaluation of three rapid methods for the direct identification of Staphylococcus aureus from positive blood cultures. J. Clin. Microbiol. 41: 4324-4327   DOI
35 Nawaz, M. S., A. A. Khan, S. A., Khan, D. D. Paine, J. V. Pothuluri., and C. E. Cerniglia. 1998. Biochemical and molecular characterization of erythromycin resistant avian Staphylococcus spp. isolated from chickens. Poult. Sci. 78: 1191-1197
36 Song, J. H. 1999. Emerging infectious disease due to microbial adaptation: emergence and spread of antimicrobial resistance. Kor. J. Infect. Dis. 31: 79-87
37 Stender, H., A. J. Broomer, K. Oliveira, H. Perry-O'Keefe, J. J. Hyldig-Nielsen, A. Sage, and J. Coulll. 2001. Rapid detection, identification, and enumeration of Escherichia coli cells in municipal water by chemiluminescent In Situ hybridization. Appl. Environ. Microbiol. 67: 142-147   DOI   ScienceOn
38 Cheng, S., F. K. McCleskey, M. J. Gress, J. M. Petroziello, R. L. Namdari, K. Beninga, A. Salmen, and V. G. DelVecchio. 1997. A PCR assay for identification of Enterococcus faecium. J. Clin. Microbiol. 35: 1248-1250   PUBMED