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http://dx.doi.org/10.7845/kjm.2013.3030

Genetic Properties and Antimicrobial Resistance of Campylobacter jejuni Isolates from Diarrhea Patients in Gyeonggi-do  

Hur, Eun-Seon (Team of Microbiology, Gyeonggi Institute of Health and Environment)
Park, Po-Hyun (Team of Microbiology, Gyeonggi Institute of Health and Environment)
Kim, Jong-Hwa (Team of Microbiology, Gyeonggi Institute of Health and Environment)
Son, Jong-Sung (Team of Microbiology, Gyeonggi Institute of Health and Environment)
Yun, Hee-Jeong (Team of Microbiology, Gyeonggi Institute of Health and Environment)
Lee, Yea-Eun (Team of Microbiology, Gyeonggi Institute of Health and Environment)
Choi, Yun-Sook (Team of Microbiology, Gyeonggi Institute of Health and Environment)
Yoon, Mi-Hye (Team of Microbiology, Gyeonggi Institute of Health and Environment)
Lee, Jong-Bok (Team of Microbiology, Gyeonggi Institute of Health and Environment)
Publication Information
Korean Journal of Microbiology / v.49, no.3, 2013 , pp. 228-236 More about this Journal
Abstract
Campylobacter jejuni is one of important food-borne pathogens causing human gastroenteritis. We isolated 42 strains of C. jejuni from diarrhea patients and 4 food-poisoning outbreaks in 2010, Gyeonggi-do. In this study, 42 strains were tested for genetic characteristics, the serotype distribution and antimicrobial resistant rate. The presence of hipO (100%), cdtB (100%), and mutated gyrA (95.2%) genes was detected in C. jejuni by polymerase chain reaction (PCR). Detection of mutated gyrA gene correlated with ciprofloxacin resistance. Forty isolates had mutated gyrA gene and were actually resistant to ciprofloxacin. Furthermore, comparing the gyrA DNA sequence data, ciprofloxacin-resistant isolates had a mutation of the DNA sequence from ACA (threonine) to ATA (isoleucine). But 41 strains (97.6%) of patient isolates were susceptible to erythromycin and azithromycin. A total of 35.7% among 42 C. jejuni isolates were identified into 4 different serotypes. The serotype distribution of C. jejuni strains were shown to be HS2(B), HS3(C), HS4(D), HS19(O). To investigate the genotypes of C. jejuni isolated in Gyeonggi province, repetitive sequence polymerase chain reaction (rep-PCR) analysis and SmaI-digested pulsed-filed gel electrophoresis (PFGE) profile analysis were performed. From the PFGE analysis of 42 C. jejuni strains, 12 clusters of PFGE profile were obtained. On the other hand, 11 clusters of rep-PCR profile were obtained from 42 strains of C. jejuni.
Keywords
Campylobacter jejuni; gyrA; antimicrobial susceptibility; PFGE; rep-PCR;
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1 Wilson, M.K., Lane, A.B., Law, B.F., Miller, W.G., Joens, L.A., Konkel, M.E., and White, B.A. 2009. Analysis of the pan genome of Campylobacter jejuni isolates recovered from poultry by pulsed-field gel electrophoresis, multilocus sequence typing (MLST), and repetitive sequence polymerase chain reaction (rep-PCR) reveals different discriminatory capabilities. Microb. Ecol. 58, 843-855.   DOI
2 Zaman, R. 1992. Campylobacter enteritis in Saudi Arabia. Epidemiol. Infect. 108, 51-58.   DOI
3 Zhao, C., Ge, B., De Villena, J., Sudler, R., Yeh, E., Zhao, S., White, D.G., Wagner, D., and Meng, J. 2001. Prevalence of Campylobacter spp., Escherichia coli, and Salmonella serovars in retail chicken, turkey, pork, and beef from the Greater Washington, D.C., area. Appl. Environ. Microbiol. 67, 5431-5436.   DOI   ScienceOn
4 Zirnstein, G., Li, Y., Swaminathan, B., and Angulo, F. 1999. Ciprofloxacin resistance in Campylobacter jejuni isolates: detection of gyrA resistance mutations by mismatch amplification mutation assay PCR and DNA sequence analysis. J. Clin. Microbiol. 37, 3276-3280.
5 Piddock, L.J. 1995. Quinolone resistance and Campylobacter spp. J. Antimicrob. Chemother. 36, 891-898.   DOI
6 Purdy, D., Buswell, C.M., Hodgson, A.E., McAlpine, K., Henderson, I., and Leach, S.A. 2000. Characterisation of cytolethal distending toxin (CDT) mutants of Campylobacter jejuni. J. Med. Microbiol. 49, 473-479.   DOI
7 Ruiz, J., Goni, P., Marco, F., Gallardo, F., Mirelis, B., Jimenez De Anta, T., and Vila, J. 1998. Increased resistance to quinolones Campylobacter jejuni: a genetic analysis of gyrA gene mutations in quinolone-resistant clinical isolates. Microbiol. Immunol. 42, 223-226.   DOI
8 Snchez, R., Fernndez-Baca, V., Daz, M.D., Muoz, P., Rodrguez-Crixems, M., and Bouza, E. 1994. Evolution of susceptibilities of Campylobacter spp. to quinolones and macrolides. Antimicrob. Agents Chemother. 38, 1879-1882.   DOI
9 Takahashi, M., Koga, M., Yokoyama, K., and Yuki, N. 2005. Epidemiology of Campylobacter jejuni isolated from patients with Guillain-Barre and Fisher syndromes in Japan. J. Clin. Microbiol. 43, 335-339.   DOI   ScienceOn
10 Talukder, K.A., Aslam, M., Islam, Z., Azmi, I.J., Dutta, D.K., Hossain, S., Nur, E.K.A., Nair, G.B., Cravioto, A., and Sack, D.A. 2008. Prevalence of virulence genes and cytolethal distending toxin production in Campylobacter jejuni isolates from diarrheal patients in Bangladesh. J. Clin. Microbiol. 46, 1485-1488.   DOI   ScienceOn
11 Vanhoof, R., Vanderlinden, M.P., Dierickx, R., Lauwers, S., Yourassowsky, E., and Butzler, J.P. 1978. Susceptibility of Campylobacter fetus subsp. jejuni to twenty nine antimicrobial agents. Antimicrob. Agents Chemother. 14, 553-556.   DOI   ScienceOn
12 Walder, M. 1979. Susceptibility of Campylobacter fetus subsp. jejuni to twenty antimicrobiol agents. Antimicrob. Agents Chemother. 16, 37-39.   DOI   ScienceOn
13 Wang, G., Clark, C.G., Taylor, T.M., Pucknell, C., Barton, C., Price, L., Woodward, D.L., and Rodgers, F.G. 2002. Colony multiplex PCR assay for identification and differentiation of Campylobacter jejuni, Campylobacter coli, Campylobacter lari, Campylobacter upsaliensis, and Campylobacter fetus subsp. fetus. J. Clin. Microbiol. 40, 4744-4747.   DOI   ScienceOn
14 Endtz, H.P., Ruijs, G.J., van Klingeren, B., Jansen, W.H., van der Reyden, T., and Mouton, R.P. 1991. Quinolone resistance in Campylobacter isolated from man and poultry following the introduction of fluoroquinolones in veterinary medicine. J. Antimicrob. Chemother. 27, 199-208.   DOI
15 Gaunt, P.N. and Piddock, L.J. 1996. Ciprofloxacin resistant Campylobacter spp. in humans: an epidemiological and laboratory study. J. Antimicrob. Chemother. 37, 747-757.   DOI   ScienceOn
16 Gootz, T.D. and Martin, B.A. 1991. Characterization of high-level quinolone resistance in Campylobacter jejuni. Antimicrob. Agents Chemother. 35, 840-845.   DOI   ScienceOn
17 Johnson, W.M. and Lior, H. 1988. A new heat-labile cytolethal distending toxin (CLDT) produced by Campylobacter spp. Microb. Pathog. 4, 115-126.   DOI   ScienceOn
18 Lee, J.K., Kim, K.Y., Koo, M.S., Yong, D.E., and Kim, E.C. 2002. Detection of Campylobacter jejuni by multiplex PCR and patterns of pulsed-field gel electrophoresis. Kor. J. Clin. Microbiol. 5, 35-41.
19 Kim, J.M., Hong, J., Bae, W., Koo, H.C., Kim, S.H., and Park, Y.H. 2010. Prevalence, antibiograms, and transferable tet(O) plasmid of Campylobacter jejuni and Campylobacter coli isolated from raw chicken, pork, and human clinical cases in Korea. J. Food Prot. 73, 1430-1437.   DOI
20 Kim, S.M., Kim, E.C., Choi, M.R., So, H.A., Shim, E.S., Kim, E.S., Park, S.C., Seong, C.N., and Chong, Y. 2008. Cytolethal distending toxin production, genotypes and antimicrobial susceptibility of Campylobacter jejuni isolates from diarrhea patients and chickens. J. Bacteriol. Virol. 38, 207-219.   DOI   ScienceOn
21 Lindmark, B., Rompikuntal, P.K., Vaitkevicius, K., Song, T., Mizunoe, Y., Uhlin, B.E., Guerry, P., and Wai, S.N. 2009. Outer membrane vesicle-mediated release of cytolethal distending toxin (CDT) from Campylobacter jejuni. BMC Microbiol. 9, 220.   DOI   ScienceOn
22 Park, E.H., Kim, J.A., Choi, S.H., Bin, J.H., Cheigh, H.S., Suk, D.H., Lee, S.C., and Kim, Y.H. 2007. Isolation and antimicrobial susceptibility of Campylobacter Jejuni from diarrhea patients. J. Life Sci. 17, 811-815.   과학기술학회마을   DOI   ScienceOn
23 Choi, K.M., Lee, J.H., Park, Y.K., Kim, N.R., Soo, H.A., Jang, Y.M., Kim, C.H., Na, S.E., Lim, S.C., and Park, M.Y. 2008. Genomic characterization and antibiotic resistance of Campylobacter jejuni isolated from poultry, bovine and human in Jeonbuk province. The report of Jeollabukdo institute of Health & Environment Research, 99-119.
24 Aarestrup, F.M. and Wegener, H.C. 1999. The effects of antibiotic usage in food animals on the development of antimicrobial resistance of importance for humans in Campylobacter and Escherichia coli. Microbes Infect. 1, 639–644.   DOI   ScienceOn
25 Altekruse, S.F., Stern, N.J., Fields, P.I., and Swerdlow, D.L. 1999. Campylobacter jejuni-an emerging foodborne pathogen. Emerg. Infect. Dis. 5, 28-35.   DOI   ScienceOn
26 Charvalos, E., Peteinaki, E., Spyridaki, I., Manetas, S., and Tselentis, Y. 1996. Detection of ciprofloxacin resistance mutations in Campylobacter jejuni gyrA by nonradioisotopic single-strand conformation polymorphism and direct DNA sequencing. J. Clin. Lab. Anal. 10, 129-133.   DOI
27 Dassanayake, R.P., Zhou, Y., Hinkley, S., Stryker, C.J., Plauche, G., Borda, J.T., Sestak, K., and Duhamel, G.E. 2005. Characterization of cytolethal distending toxin of Campylobacter species isolated from captive macaque monkeys. J. Clin. Microbiol. 43, 641-649.   DOI   ScienceOn