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http://dx.doi.org/10.4163/kjn.2010.43.6.561

Antihypertensive Properties of Dried Radish Leaves Powder in Spontaneously Hypertensive Rats  

Kim, Bo-Ram (Department of Food & Nutrition, Kookmin University)
Park, Ji-Ho (Department of Food & Nutrition, Kookmin University)
Kim, Sun-Hee (Department of Food & Nutrition, Kookmin University)
Cho, Kang-Jin (Department of Functional Food & Nutrition Division, Rural National Academy of Agricultural Science)
Chang, Moon-Jeong (Department of Food & Nutrition, Kookmin University)
Publication Information
Journal of Nutrition and Health / v.43, no.6, 2010 , pp. 561-569 More about this Journal
Abstract
The study aim was to investigate the antihypertensive effect after oral supplementation of dried radish leaves powder (DRLP). Angiotensin-converting enzyme (ACE) activity was measured by spectrophotometric assay. The systolic blood pressure (SBP) was measured in spontaneously hypertensive (SHR) and normotensive rats (Wistar) by the tail cuff method after a 4-week diet with DRLP at the level of 2.5% or 5%. The supplementation of DRLP decreased SBP of SHR although the 5% supplementation level did not show any more pronounced effect than the 2.5% level did. The decrease in the SBP observed for both 2.5% and 5% DRLP was accompanied by significant increases of the urinary Na and K excretion. The DRLP supplementation showed a potent ACE-inhibitory activity in pulmonary tissue from both hypertensive and normotensive rats. However, the DRLP supplementation did not affect the SBP in normotensive rats. These results indicated that DRLP exerted an antihypertensive effect in SHR due to the decreased ACE activity and increased urinary Na excretion.
Keywords
dried radish leaves powder; blood pressure; angiotensin converting enzyme activities; hypertension;
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1 Chobanian AV, Bakris GL, Black HR, Cushman WC, Green LA, Izzo JL Jr, Jones DW, Materson BJ, Oparil S, Wright JT Jr, Roccella EJ. Seventh report of the joint national committee on prevention, detection, evaluation, and treatment of high blood pressure. Hypertension 2003; 42(6): 1206-1252   DOI   ScienceOn
2 DiMicco JA, Abshire VM. Evidence of GABAergic inhibition of a hypothalamic sympathoexcitatory mechanism in anesthetized rats. Brain Res 1987; 402: 1-10   DOI   ScienceOn
3 Kwan CY. Vascular effects of selected antihypertensive drugs derived from traditional medicinal herbs. Clin Exp Pharmacol Physiol 1995; 22(1, Suppl): S297-S299.   DOI
4 Sgherri C, Cosi E, Navari-Izzo F. Phenols and antioxidative status of raphanus sativus grown in copper excess. Physiol Plant 2003; 118(1): 21-28   DOI   ScienceOn
5 Dillon GH, Shonis CA, Waldrop TG. Hypothalamic GABAergic modulation of respiratory responses to baroreceptor stimulation. Respir Physiol 1991; 85(3): 289-304   DOI   ScienceOn
6 Jouad H, Lacaille-Dubois MA, Lyoussi B, Eddouks M. Effects of the flavonoids extracted from Spergularia purpurea Pers. on arterial blood pressure and renal function in normal and hypertensive rats. J Ethnopharmacol 2001; 76(2): 159-163   DOI   ScienceOn
7 Azizi M, Webb R, Nussberger J, Hollenberg NK. Renin inhibition with aliskiren: where are we now, and where are we going? J Hypertens 2006; 24(2): 243-256   DOI   ScienceOn
8 Arendshorst WJ, Chatziantoniou C, Daniels FH. Role of angiotensin in the renal vasoconstriction observed during the development of genetic hypertension. Kidney Int 1990; 30 (Suppl): S92- S96
9 Correa FMA, Viswanathan M, CIUFFO GM, Tsutsumi K, Saavedra JM. Kidney angiotensin II receptors and converting enzyme in neonatal and adult wistar-kyoto and spontaneously hypertensive rats. Peptides 1995; 16(1): 19-24   DOI   ScienceOn
10 Niwa A, Israel A, Saavedra JM. Pindolol decreases plasma angiotensin- converting enzyme activity in young spontaneously hypertensive rats. Eur J Pharmacol 1985; 110(1): 133-136   DOI   ScienceOn
11 Hagiwara Y, Kubo T. γ-Aminobutyric acid in the lateral septal area is involved in mediation of the inhibition of hypothalamic angiotensin II-sensitive neurons induced by blood pressure increases in rats. Neurosci Lett 2007; 419(3): 242-246   DOI   ScienceOn
12 Adachi N, Tomonaga S, Tachibana T, Denbow DM, Furuse M. (-)-Epigallocatechin gallate attenuates acute stress responses through GABAergic system in the brain. Eur J Pharmacol 2006; 531(1-3): 171-175   DOI
13 Qinn ZN, Song LH, Gu ZL, Chen BQ, Zhang KP, Li Hz, Peng YK. An experimental observation on the diuretic effect of an extract of Luobuma (Apocynum 6enetum) leaves. Zhong Yao Tong Bao 1988; 13(10): 44-46
14 Kim D, Yokozawa T, Hattori M, Kadota S, Namba T. Effects of aqueous extracts of Apocynum 6enetum leaves on spontaneously hypertensive, renal hypertensive and NaCl-fed-hypertensive rats. J Ethnopharmacol 2000; 72(1-2): 53-59   DOI
15 Chun OK, Chung SJ, Song WO. Estimated dietary flavonoid intake and major food sources of U.S. adults. J Nutr 2007; 137(5): 1244-1252
16 Lee HS, Chang MJ, Kim SH. Effects of poly-${\gamma}$-glutamic acid o serum and brain concentrations of glutamate and GABA in dietinduced obese rats. Nutr Res Pract 2010; 4(1): 23-29   과학기술학회마을   DOI
17 Neels HM, van Sande ME, Scharpe SL. Sensitive colorimetric assay for angiotensin converting enzyme in serum. Clin Chem 1983; 29(7): 1399-1403
18 Clarke G, O'Mahony S, Malone G, Dinan TG. An isocratic high performance liquid chromatography method for the determination of GABA and glutamate in discrete regions of the rodent brain. J Neurosci Methods 2007; 160(2): 223-230   DOI   ScienceOn
19 Vermeij TA, Edelbroek PM. Simultaneous high-performance liquid chromatographic analysis of pregabalin, gabapectin and vigabatrin in human serum by precolumn derivatization with o-phataldialdehyde an fluorescence detection. J Chromatogr B Analyt Technol Biomed Life Sci 2004; 810(2): 297-303
20 Peach MJ. Renin-angiotensin system: biochemistry and mechanisms of action. Physiol Rev 1977; 57(2); 313-370
21 Soubrier F, Wei L, Hubert C, Clauser E, Alhenc-Gelas F, Corvol P. Molecular biology of the angiotensin I-converting enzyme: II. Structure-function. Gene polymorphism and clinical implications. J Hypertens 1993; 11(6): 599-604   DOI   ScienceOn
22 de Gasparo M, Catt KJ, Inagami T, Wright JW, Unger TH. International Union of pharmacology. XXIII. The angiotensin receptors. Pharmacol Rev 2000: 52: 415-472
23 Edemann D. Touyz Rm, Li JS, Deng LY, Schiffrin EL. Altered angiotensin II-induced small artery contraction during the development of hypertension in spontaneously hypertensive rats. Am J Hypertens 1999; 12(7): 716-723   DOI   ScienceOn
24 Remuzzi G, Perico N, Macia M, Ruggenenti P. The role of renin- angiotensin-aldosterone system in the progression of chronic kidney disease, Kidney Int 2005: 99 (Suppl.): S57-S65
25 Sutter MC, Wang YX. Recent cardiovascular drugs from Chinese medicinal plants. Cardiovasc Res 1993; 27(11): 1891-1901   DOI   ScienceOn
26 Singewald N, Schneider C, Philippu A. Disturbances in blood pressure homeostasis modify GABA release in the locus coeruleus. Neuroreport 1994; 5: 1709-1712   DOI   ScienceOn
27 Waeber B, Bruner HR. A look through the new therapeutic window. J hypertens 1998; 16(Suppl): S11-S16
28 Luo LF, Wu WH, Zhou YJ, Yan J, Yang GP, Ouyang DS. Antihypertensive effect of eucommia ulmoides oliv. extracts in spontaneously hypertensive rats. J Ethnopharmacol 2010; 129(2): 238-243.   DOI   ScienceOn
29 Gillis CN. Panax ginseng pharmacology: A nitric oxide link? Biochem Pharmacol 1997; 54(1): 1-8   DOI   ScienceOn
30 Schmidtlein H, Herrmann K. On phenolic acids of vegetables. I. hydroxycinnamic acids and hydroxybenzoic acids of brassicaspecies and leaves of other cruciferae. Z Lebensm Unters Forsch 1975; 159(3): 139-148   DOI   ScienceOn
31 Ku KH, Lee KA, Kim YE. Physiological activity of extracts from radish (raphanus sativus L.) leaves. J Korean Soc Food Sci Nutr 2008; 37(3): 390-395   과학기술학회마을   DOI
32 Han JS, Kim JS, Kim MS, Choi YH, Minamide T, Huh SM. Changes on mineral contents of vegetable by various cooking methods. Korean J Soc Food Sci 1999; 15: 382-387
33 Ghayur MN, Gilani AH. Gastrointestinal stimulatory and uterotonic activities of dietary radish leaves extract are mediated through multiple pathways. Phytother Res 2005; 19(9): 750-755   DOI   ScienceOn
34 Gilani AH, Ghayur MN. Pharmacological basis for the gut stimulatory activity of raphanus sativus leaves. J Ethnopharmacol 2004; 95(2-3): 169-172   DOI   ScienceOn
35 Ministry of Health and Welfare, KHANES IV 2nd year report; 2008, p.50
36 Marinova D, Ribarova F, Atanassova M. Total phenolics and total flavonoids in Bulgarian fruits and vegetables. J Univ Chem Technol Metallurgy 2005; 40(3): 255-260
37 Kearney PM, Whelton M, Reynolds K, Muntner P, Whelton PK, He J. Global burden of hypertension: Analysis of worldwide data. Lancet 2005; 365: 217-223
38 Ezzati M, Lopez AD, Rodgers A, Vander Hoorn S, Murray CJ, Comparative Risk Assessment Collaborating Group. Selected major risk factors and global and regional burden of disease. Lancet 2002; 360: 1347-1360   DOI
39 Ames RP. Insights from Laragh's review course: the role of the renin-angiotensin system in blood pressure regulation. Am J Hypertens 2001; 15(7): 653-654
40 Saito Y, Nakamura K, Kawato K, Imayasu S. Angiotensin I converting enzyme inhibitors in sake and its by-products. Nippon Nogeikagaku Kaishi 1992; 66: 1081-1087   DOI   ScienceOn
41 Okamoto A, Hanagata H, Matsumoto E, Kawamura Y, Koizumi Y, Yanagida F. Angiotensin I converting enzyme inhibitory activities of various fermented foods. Biosci Biotechnol Biochem 1995; 59: 1147-1149   DOI   ScienceOn
42 Hagiwara Y, Kubo T.γ-Aminobutyric acid in the lateral septal area is involved in mediation of the inhibition of hypothalamic angiotensin II sensitive neurons induced by blood pressure increases in rats. Neurosci Lett 2007; 419: 242-246   DOI   ScienceOn
43 Shonis CA, Waldrop TG. Augmented neuronal acitivity in the hypothalamus of spontaneously rats. Brain Res Bull 1993; 30: 45-52   DOI   ScienceOn
44 Shonis CA, Peano CA, Dillon GH, Waldrop TG. Cardiovascular response to blockade of GABA synthesis in the hypothalamus of the spontaneously hypertensive rat. Brain Res Bull 1993; 31: 493- 499   DOI   ScienceOn