Browse > Article
http://dx.doi.org/10.7732/kjpr.2022.35.3.391

The Beneficial Effect of Platycodon grandiflorum on DSS-induced Colitis through Regulation of HIF-1α in Mice  

Yang, Mi-Ok (Wonkwang Institute of Nature Healthcare Studies)
Myung, Noh-Yil (Department of Oriental Medicine and Healthcare, Wonkwang Digital University)
Publication Information
Korean Journal of Plant Resources / v.35, no.3, 2022 , pp. 391-398 More about this Journal
Abstract
Ulcerative colitis (UC) is a typical inflammatory colon disorder. Platycodon grandiflorum (PG) is known to exert various beneficial effects including anti-oxidative and anti-bacterial properties and improvements in liver function. However, the improving effect and mechanism of PG on intestinal inflammation are not fully understood. The present research was designed to investigate the effect of PG on the clinical signs of DSS-induced colitis in mice. The ameliorative effects of PG on inflammatory cytokine expression and the activation of hypoxia-inducible-factor (HIF)-1α in DSS-treated colon tissue were also determined. Our results showed that mice treated with DSS displayed the main clinical symptoms of colitis, including weight loss, bloody stools, decrease in colon length and diarrhea and PG treatment significantly improved the clinical features induced by DSS in mice. PG inhibited the increase in the levels of inflammatory cytokines caused by DSS in colon tissues. We also showed that the anti-inflammatory mechanism of PG involved suppressing the activation of HIF-1α in DSS-treated colon tissues. Collectively, the findings of this study indicate the prospect of developing new drugs from PG for UC treatment.
Keywords
Dextran sulfate sodium; Hypoxia-inducible-factor (HIF)-$1{\alpha}$; Inflammatory cytokine; Platycodon grandiflorum; Ulcerative colitis;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Park, Y.S., Y.S. Yoon and H.S. Ahn. 2007. Platycodon grandiflorum extract represses up-regulated adipocyte fatty acid binding protein triggered by a high fat feeding in obese rats. World J. Gastroenterol. 7:3493-3499.   DOI
2 Mueller, C. 2002. Tumour necrosis factor in mouse models of chronic intestinal inflammation. Immunology 105:1-8.   DOI
3 Sandborn, W.J. and S.R. Targan. 2002. Biologic therapy of inflammatory bowel disease. Gastroenterology 122:1592-1608.   DOI
4 Scaldaferri, F., S. Vetrano, M. Sans, V. Arena, G. Straface, E. Stigliano, A. Repici, A. Sturm, A. Malesci, J. Panes, S. Yla-Herttuala, C. Fiocchi and S. Danese. 2009. VEGF-A links angiogenesis and inflammation in inflammatory bowel disease pathogenesis. Gastroenterology 136:585-595.   DOI
5 Taylor, C.T. and S.P. Colgan. 2007. Hypoxia and gastrointestinal disease. J. Mol. Med. 85:1295-1300.   DOI
6 Wirtz, S., V. Popp, M. Kindermann, K. Gerlach, B. Weigmann, S. Fichtner-Feigl and M.F. Neurath. 2017. Chemically induced mouse models of acute and chronic intestinal inflammation. Nat. Protoc. 12(7):1295-1309.   DOI
7 Yashiro, M. 2014. Ulcerative colitis-associated colorectal cancer. World J. Gastroenterol. 28:16389-16397.   DOI
8 Glover, L.E. and S.P. Colgan. 2011. Hypoxia and metabolic factors that influence inflammatory bowel disease pathogenesis. Gastroenterology 140:1748-1755.   DOI
9 Ardizzone, S. and P.G. Bianchi. 2005. Biologic therapy for inflammatory bowel disease. Drugs 65:2253-2286.   DOI
10 Cottone, M., S. Renna, I. Modesto and A. Orlando. 2011. Is 5-ASA still the treatment of choice for ulcerative colitis? Curr. Drug Targets 12:1396-1405.   DOI
11 Eichele, D.D. and K.K. Kharbanda. 2017. Dextran sodium sulfate colitis murine model: An indispensable tool for advancing our understanding of inflammatory bowel diseases pathogenesis. World J. Gastroenterol. 23:6016-6029.   DOI
12 Hendrickson, B.A., R. Gokhale and J.H. Cho. 2002. Clinical aspects and pathophysiology of inflammatory bowel disease. Clin. Microbiol. Rev. 15:79-94.   DOI
13 Kim, Y.S. J.S. Kim, S.U. Choi, J.S. Kim, H.S. Lee, S.H. Roh, Y.C. Jeong, Y.K. Kim and S.Y. Ryu. 2005. Isolation of a new saponin and cytotoxic effect of saponins from the root of Platycodon grandiflorum on human tumor cell lines. Planta Med. 71:566-568.   DOI
14 Nunes, N.S., S. Kim, M. Sundby, P. Chandran, S.R. Burks, A.H. Paz and J.A. Frank. 2018. Temporal clinical, proteomic, histological and cellular immune responses of dextran sulfate sodium-induced acute colitis. World J. Gastroenterol. 24(38):4341-4355.   DOI
15 Park, J.H., L. Peyrin-Biroulet, M. Eisenhut and J.I. Shin. 2017. IBD immunopathogenesis: A comprehensive review of inflammatory molecules. Autoimmun. Rev. 16(4):416-426.   DOI
16 Sands, B.E. and G.G. Kaplan. 2007. The role of TNFalpha in ulcerative colitis. J. Clin. Pharmacol. 47(8):930-941.   DOI
17 Van Uden, P., N.S. Kenneth and S. Rocha. 2008. Regulation of hypoxia-inducible factor-1α by NF-κB. Biochem. J. 412: 477-484.   DOI
18 Danese, S., M. Sans and C. Fiocchi. 2004. Inflammatory bowel disease; the role of environmental factors. Autoimmun. Rev. 3:394-400.   DOI
19 Baumgart, D.C. and S.R. Carding. 2007. Inflammatory bowel disease: Cause and immunobiology. Lancet 369:1627-1640.   DOI
20 Choi, J.N. and W.H. Lee. 2018. An investigation of characteristics of Chinese bellflower (Platycodon grandiflorum A.) cultivated soil. Korean J. Plant Res. 31(6):660-666.   DOI
21 Domenech, E. 2006. Inflammatory bowel disease: Current therapeutic options. Digestion 73:67-76.   DOI
22 Furuta, G.T., J.R. Turner, C.T. Taylor, R.M. Hershberg, K. Comerford, S. Narravula, D.K. Podolsky and S.P. Colgan. 2001. Hypoxia-inducible factor 1-dependent induction of intestinal trefoil factor protects barrier function during hypoxia. J. Exp. Med. 193(9):1027-1034.   DOI
23 Ogata, H. and T. Hibi. 2003. Cytokine and anti-cytokine therapies for inflammatory bowel disease. Curr. Pharm. Des. 9:1107-1113.   DOI
24 Karhausen, J., G.T. Furuta, J. E. Tomaszewski, R. S. Johnson, S.P. Colgan and V.H. Haase. 2004. Epithelial hypoxia-inducible factor-1 is protective in murine experimental colitis. J. Clin. Investig. 8:1098-1106.
25 Kim, Y.P., E.B. Lee, S.Y. Kim, D. Li, H.S. Ban, S.S. Lim, K.H. Shin and K. Ohuchi. 2001. Inhibition of prostaglandin E2 production by platycodin D isolated from the root of Platycodon grandiflorum. Planta Med. 67:362-364.   DOI
26 Li, Y., C. de Haar, M Chen, J. Deuring, M.M. Gerrits, R. Smits, B. Xia, E.J. Kuipers and J. van der Woude. 2010. Disease-related expression of the IL-6/STAT3/SOCS3 signaling pathway in ulcerative colitis and ulcerative colitis-related carcinogenesis. Gut. 59:227-235.   DOI
27 Papadakis, K.A. and S.R. Targan. 2000. Role of cytokines in the pathogenesis of inflammatory bowel disease. Annu. Rev. Med. 51:289-298.   DOI