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http://dx.doi.org/10.7732/kjpr.2012.25.2.184

Cytoprotective Effect of Ethanol Extract from Maesil (Prunus mume Sieb. et Zucc.) on Alloxan-induced Oxidative Damage in Pancreatic-cell, HIT-T15  

Kim, In-Hye (Functional food & Nutrition Division, National Academy of Agricultural Science, Rural Development Administration)
Kim, Jong-Bae (Department of Food and Nutrition, Hanyang University)
Cho, Kang-Jin (Functional food & Nutrition Division, National Academy of Agricultural Science, Rural Development Administration)
Kim, Jae-Hyun (Functional food & Nutrition Division, National Academy of Agricultural Science, Rural Development Administration)
Om, Ae-Son (Department of Food and Nutrition, Hanyang University)
Publication Information
Korean Journal of Plant Resources / v.25, no.2, 2012 , pp. 184-192 More about this Journal
Abstract
The present study was designed to examine the potential antidiabetic and antioxidant effect of ethanol extract from $Prunus$ $mume$ fruit (PME) against alloxan-induced oxidative stress in pancreatic ${\beta}$-cells, HIT-T15. To evaluate the antidiabetic effect of PME, 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliu bromide (MTT) cell proliferation assay, lactate dehydrogenase (LDH) release assay, $NAD^+$/NADH ratio and insulin secretion were assessed. We also measured its antioxidant effect against alloxan-induced oxidative stress in the cells by assessing the levels of the antioxidant enzymes including superoxide dismutase (SOD), glutathione S-transferase (GST), glutathione reductase (GR) and glutathione peroxidase (GPx). The results of this analysis showed that alloxan significantly decreased cell viability, increased LDH leakage, and lowered $NAD^+$ /NADH ratio and insulin secretion in HIT-T15 cells. However, PME significantly increased the viability of alloxan-treated cells and lowered LDH leakage. The intracellular $NAD^+$ /NADH ratio and insulin secretion were also increased by 1.5~1.9-fold and 1.4-fold, respectively, after treatment with the PME. The HIT-T15 cells treated with alloxan showed significant decreases in the activities of antioxidant enzymes, while PME significantly elevated the levels of antioxidant enzymes. Based on these results, we suggest that PME could have a protective effect against the cytotoxicity and dysfunction of pancreatic ${\beta}$-cells in the presence of alloxan-induced oxidative stress.
Keywords
Prunus mume; Antidiabetes; Antioxidant; Alloxan; HIT-T15 cell;
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1 Ramkumar K.M, C. Manjula, L. Sankar, S. Suriyanarayanan and P. Rajaguru. 2009. Potentail in vitro antioxidant and protective effects of Gymnema montanum H. on alloxaninduced oxidative damage in pancreatic $\beta$-cells, HIT-T15. Food Chem. Toxicol. 47:2246-2256   DOI
2 Rho, H.W., J.N. Lee, H.R. Kim, B.H. Park and J.W. Park. 2000. Protective mechanism of glucose against alloxaninduced $\beta$-cell damage: Pivotal role of ATP. Exp. Mol. Med. 21:12-17.
3 Seo, K.S., C.K. Huh and Y.D. Kim. 2008. Changes of biologically active components in Prunus mume fruit. Korean J. Food. Preserv. 15:269-273 (in Korean).   과학기술학회마을
4 Seneviratne C.J., R.W. Wong, U., Hgg, Y. Chen, T.D. Herath, P.L. Samaranayake and R. Kao. 2011. Prunus mume extract exhibits antimicrobial activity against pathogenic oral bacteria. Int. J. Paediatr. Dent. 21:299-305.   DOI
5 Winterbourn, C.C. W.B. Cowden and H.C. Sutton. 1989. Auto-oxidation of dialuric acid, divicine and isouramil. Superoxide dependent and independent mechanisms. Biochem. Pharmacol. 38:611-618.   DOI
6 Winterbourn, C.C. and R. Munday. 1989. Glutathionemediated redox cycling of alloxan. Mechanisms of superoxide dismutase inhibition and of metal-catalyzed OH. formation. Biochem. Pharmacol. 38:271-277.   DOI
7 Yun, H.J., I. Kim, S.H. Kwon, J.S. Kang and A.S. Om. 2011. Protective effect of Chlorella vulgaris against leadinduced oxidative stress in rat brains. J. Health. Sci. 57:245-254.   DOI
8 Malaisse W.J., F. MALAISSE-Lagae, A. Sener and D.G. Pipeleers. 1982. Determinants of the selective toxicity of alloxan to the pancreatic $\beta$ cell. Proc. Natl. Acad. Sci. 79:927-930.   DOI   ScienceOn
9 Montserrat, M., A. Moreles, A. Colell, C. Garcia-Ruiz and J.C. Fernandez-Checa. 2009. Mitochondiral glutathione, a key survival antioxidant. ANTIOXID. REDOX. SIGN. 11:2685-2700.   DOI
10 Moon, E.J., Y.E. Jo, T.C. Park, Y.K. Kim, S.H. Jung, H.J. Kim, D.J. Kim, Y.S. Chung and K.W. Lee. 2008. Clinical characteristics and direct medical costs of type 2 diabetic patients. Korean Diabetes. J. 32:358-365 (in Korean).   과학기술학회마을   DOI   ScienceOn
11 Park, I.B. and S.H. Baik. 2009. Epidemilogic characteristics of diabetes mellitus in Korea: Current status of diabetic patients using Korean Health Insurance Database. Korean Diabetes. J. 33:357-362 (in Korean).   DOI
12 Munday, R. 1988. Dialuric acid autoxidation-Effects of transition metals on the reaction rate and on the generation of 'active oxygen' species. Biochem. Pharmacol. 37:409-413.   DOI
13 Okamoto, H. 1985. Molecular basis of experimental diabetes: Degeneration, oncogenesis and regeneration of pancreatic $\beta$-cells of islet of Langerhans. Bioassay 2:15-21.   DOI
14 Park, T.S., T.H. Lee and H.R. Kim. 1999. Protective mechanism of glucose against alloxan-induced HIT-T15 cell damage. Korean Diabetes. J. 23:530-540 (in Korean).
15 Park, Y.M., J.B. Jeong, J.H. Seo, J.H. Lim, H.J. Jeong and E.W. Seo. 2011. Inhibitory effect of red bean (Phaseolus angularis) hot water extracts on oxidative DNA and cell damage. Korean J. Plant Res. 24:130-138 (in Korean).   과학기술학회마을   DOI
16 Jones, D.P., L. Eklow and S. Orrenius. 1981. Metabolism of hydrogen peroxide in isolated hepatocytes: relative contribution of catalase and gluthathione peroxidase in decomposion of endogenously generated $H_{2}O_{2}$. Arch. Biochem. Biophys. 210:505-516.   DOI   ScienceOn
17 Jung H.K., Y.S. Jeong, C.D. Park, C.H. Park and J.H. Hong. 2010. Effect of the ethanol extract from citrus peels on oxidative damage in alloxan-induced HIT-T15 cell. J. Korean Soc. Food. Sci. Nutr. 39:1102-1106 (in Korean).   과학기술학회마을   DOI
18 Kim Y.S. 2008. Chracteristics of diabetes in Korean population. J. Korean Med. Assoc. 51:799-805 (in Korean).   DOI
19 Lee, J.H., M.S. Na and M.Y. Lee. 2004. Effects of ethanol extract of Prunus mume on the antioxidative system and lipid peroxidation on ethanol-induced hepatotoxicity in rat liver. Korean J. Food. Preserv. 11:71-78 (in Korean).   과학기술학회마을
20 Kim, S.G. and D.S. Choi. 2009. Epidemiology and current status of diabetes in Korea. Hanyang Medical Reviews 29:122-129 (in Korean).
21 Lee, J.H., J.W. Park, J.S. Kim, B.H. Park and H.W. Rho. 2008. Protective effect of Amomi semen extract on alloxan -induced pancreatic $\beta$-cell damage. Phytother. Res. 22:86 -90.   DOI   ScienceOn
22 Lee, S.Y., U.Y. Kang, S.H. Park, S.K. Jung, K.W. Kang, T.C. Jeong, H.S. Kim, H.G. Jeong, B.H. Kim and S.K. Kim. 2009. Evaluation of hepatic antioxidant defense systems in rats treated with tetrabromobisphenol-A. J. Environ. Toxicol. 24:303-309 (in Korean).   과학기술학회마을
23 Lenzen, S. 2008. The mechanisms of alloxan- and streptozotocin -induced diabetes. Diabetologia 51:216-226.   DOI
24 Lupi, R. and S. Del-Prato. 2008. Beta-cell apoptosis in type 2 diabetes: quantitative and functional consequences. Diabetes. Metab. 34:S56-64.   DOI
25 Chung, Y.J., C.P. Park, Y.K. Jeong and Y.H. Choi. 2011. Apoptosis induction by methanol extract on Prunus mume fruits in human leukemia U937 cells. J. Life. Sci. 21: 1109-1119 (in Korean).   과학기술학회마을   DOI
26 Cohen, G. and R.E. Heikkila. 1974. The generation of hydrogen peroxide, superoxide radical, and hydroxyl radical by 6-hydroxydopamine, dialuric acid, and related cytotoxic agents. J. Biol. Chem. 249:2447-2452.
27 Enomoto, S., K, Yanaoka, H. Utsunomiya, T. Niwa, K. Inada, H. Deguchi, K. Ueda, C. Mukoubayashi, I. Inoue, T. Maekita, K. Nakazawa, M. Iguchi, K. Arii, H. Tamai, N. Yoshimura, M. Fujishiro, M. Oka and M. Ichinose. 2010. Inhibitory effects of Japanese apricot (Prunus mume Siebold et Zucc.; Ume) on Helicobacter pylorirelated chronic gastritis. European J. Clin. Nutr.64:714-719.   DOI   ScienceOn
28 De La Pea, V.A., P. Diz Dios and R. Tojo Sierra. 2007. Relationship between lactate dehydrogenase activity in saliva and oral health status. Arch. Oral. Biol. 52:911-915.   DOI
29 Doh, E.S., J.P. Chang, K.J. Kil, M.S. Choi, J.K. Yang, C.W. Yun, S.M. Jeong, Y.H. Jung and G.H. Lee. 2011. Antioxidant activity and cytotoxicity of fermented Allium victorialis L. extract. Korean J. Plant Res. 24:30-39 (in Korean).   DOI
30 Donath M.Y., J.A. Ehses, K. Maedler, D.M. Schumann, H. Ellingsgaard, E. Eppler and M. Reinecke. 2005. Mechanisms of $\beta$-cell death in type 2 diabetes. Diabetes 54:S108-S113.   DOI   ScienceOn
31 Gao, M., Y. Li and J. Yang. 2011. Protective effect of Pedicularis decora Franch root extracts on oxidative stress and hepatic injury in alloxan-induced diabetic mice. J. Med. Plants Res. 5:5848-5856.
32 Han, J.T., Y.S. Lee, K.N. Kim and N.I. Baek. 2001. Rutin, Antioxidant Compound Isolated from the Fruit of Prunus memu. J. Korean. Soc. Agric. Chem. Biotechnol. 44:35-37 (in Korean).
33 Choi, D. and M. Woo. 2010. Executioners of apoptosis in pancreatic β-cells: not just for cell death. American J. Physiol. Endocrinol. Metab. 298:E735-741.   DOI
34 Butler, A.E., J. Janson, S. Bonner-Weir, R. Ritzel, R.A. Rizza and P.C. Butler. 2003. Beta-cell deficit and increased beta-cell apoptosis in humans with type 2 diabetes. Diabetes 52:102-110.   DOI