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http://dx.doi.org/10.4490/algae.2014.29.4.355

Fucoxanthin derivatives from Sargassum siliquastrum inhibit matrix metalloproteinases by suppressing NF-κB and MAPKs in human fibrosarcoma cells  

Nguyen, Van-Tinh (Department of Biomedical Engineering, and Centre for Marine-Integrated Biomedical Technology (BK21 Plus) Pukyong National University)
Qian, Zhong-Ji (College of Food Science and Technology, Guangdong Ocean University)
Lee, Bonggi (College of Pharmacy, Pusan National University)
Heo, Soo-Jin (Global Bioresources Research Center, Korea Institute of Ocean Science and Technology)
Kim, Kil-Nam (Marine Bio Research Team, Korea Basic Science Institute (KBSI))
Jeon, You-Jin (Department of Marine Life Sciences, Jeju National University)
Park, Won Sun (Department of Physiology, Kangwon National University School of Medicine)
Choi, Il-Whan (Department of Microbiology, Inje University College of Medicine)
Jang, Chul Ho (Department of Otolaryngology, Chonnam National University Medical School)
Ko, Seok-Chun (Institute of Marine Biotechnology, Pukyong National University)
Park, Sun-Joo (Department of Chemistry, Pukyong National University)
Kim, Yong-Tae (Department of Food Science and Biotechnology, Kunsan National University)
Kim, GeunHyung (Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University)
Lee, Dae-Sung (Marine Biodiversity Institute of Korea)
Yim, Mi-Jin (Marine Biodiversity Institute of Korea)
Je, Jae-Young (Department of Marine-bio Convergence Science, Pukyong National University)
Jung, Won-Kyo (Department of Biomedical Engineering, and Centre for Marine-Integrated Biomedical Technology (BK21 Plus) Pukyong National University)
Publication Information
ALGAE / v.29, no.4, 2014 , pp. 355-366 More about this Journal
Abstract
Fucoxanthin is known to be an effective cell proliferation inhibitor with anti-tumor and anti-angiogenic activities. However, there is a lack of data regarding the biological effects of cis isomers of fucoxanthin. To assess the potential therapeutic properties of 9'-cis-(6'R) fucoxanthin (FcA), and 13-cis and 13'-cis-(6'R) fucoxanthin complex (FcB) isolated from Sarggassum siliquastrum, we investigated their inhibitory effects on matrix metalloproteinases (MMPs) in phorbol 12-myristate 13-acetate (PMA)-induced human fibrosarcoma (HT1080) cells. FcA and FcB reduced MMP-2 and MMP-9 protein and mRNA levels, as well as the migration of these cells, in a dose-dependent manner. Additionally, FcA and FcB increased levels of MMPs inhibition factors such as tissue inhibitor of metalloproteinase-1. FcA and FcB significantly inhibited the transcriptional activity of nuclear factor ${\kappa}B$ (NF-${\kappa}B$) and by inhibiting c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinases. Our results demonstrate that suppression of the NF-${\kappa}B$, JNK, and p38 signaling pathways may inhibit PMA-induced MMP-2 and MMP-9 activity. Therefore, FcA and FcB may be useful in noninvasive therapeutic strategies against fibrosarcoma metastasis.
Keywords
fucoxanthin; human fibrosarcoma cells (HT1080); matrix metalloproteinases (MMPs); mitogen-activated protein kinase (MAPK); nuclear factor-kappaB (NF-${\kappa}B$); Sargassum siliquastrum;
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