Browse > Article
http://dx.doi.org/10.6564/JKMRS.2017.21.3.085

Identification of Enzymatic Catalysis of PncA using 1H-NMR  

Yi, Jong-Jae (College of Pharmacy, CHA University)
Kim, Won-Je (College of Pharmacy, Seoul National University)
Rhee, Jin-Kyu (Department of Food Science and Engineering, Ewha Womans University)
Lim, Jongsoo (Discovery Technology Team, Dong-A ST Research Institute)
Lee, Bong-Jin (College of Pharmacy, Seoul National University)
Son, Woo Sung (College of Pharmacy, CHA University)
Publication Information
Journal of the Korean Magnetic Resonance Society / v.21, no.3, 2017 , pp. 85-89 More about this Journal
Abstract
Pyrazinamidase (PncA) from Mycobacterium tuberculosis is the hydrolytic enzyme (hydrolase) that can hydrolyze substrate PZA to active form pyrazoic acid (POA). To investigate hydrolytic reaction of M. tuberculosis PncA, 1D NMR spectra were monitored at various molar ratios of PncA and PZA. The line-width of PZA was changed as PncA was added into PZA with different molar ratios. These results suggested that determination of PncA enzymatic activity could potentially serve as an indirect measure of PZA susceptibility.
Keywords
Mycobacterium tuberculosis; Enzyme; Substrate; PncA; PZA; POA; NMR;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 World Health Organization, WHO report 2006 (2006)
2 British Thoracic Society, Br. J. Dis. Chest. 78, 330 (1984)   DOI
3 Y. Zhang, and D. Mitchison, Int. J. Tuberc. Lung. D. 7, 6 (2003)
4 A. Somoskovi, L. M. Parsons, and M. Salfinger, Respir. Res. 2, 164 (2001)   DOI
5 M. S. Tarshis, and W.A. Weed, Am. Rev. Tuberc. 67, 391 (1953)
6 D. W. Mc, and R. Tompsett, Am. Rev. Tuberc. 70, 748 (1954)
7 K. Konno, F. M. Feldmann, and W. McDermott, Am. Rev. Respir. Dis. 95, 461 (1967)
8 Y. Zhang, A. Scorpio, H. Nikaido, and Z. Sun, J. Bacteriol. 181, 2044 (1999)
9 C. Raynaud, M. A. Laneelle, and R. H. Senaratne, Microbiology 145, 1359 (1999)   DOI
10 Y. Zhang, and D. Young, J. Antimicrob. Chemoth. 34, 313 (1994)   DOI
11 A. Scorpio, and Y. Zhang, Nat. Med. 2, 662 (1996)   DOI
12 N. Lemaitre, I. Callebaut, F. Frenois, V. Jarlier, and W. Sougakoff, Biochem. J. 353, 453 (2001)
13 X. Du, W. Wang, R. Kim, H. Yakota, H. Nguyen, and S. Kim, Biochemistry 40, 14166 (2001)   DOI
14 J. Yi, J. K. Yoo, J. K. Kim, and W. S. Son, J. Kor. Magn. Reson. Soc. 17, 30 (2013)   DOI
15 W. Kim, J. Rhee, J. Yi, B. Lee, and W. S. Son, J. Kor. Magn. Reson. Soc. 18, 36 (2014)   DOI
16 D. Strack, Phytochemistry 57, 144 (2001)   DOI