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http://dx.doi.org/10.6564/JKMRS.2015.19.1.036

Backbone NMR Assignments of an Uncharacterized Protein, SF1002 from Shigella flexneri 5a M90T  

Lee, Yoo-Sup (Department of Molecular Science and Technology, Ajou University)
Yoon, Won-Su (Department of Molecular Science and Technology, Ajou University)
Chung, Il (College of Pharmacy, Ajou University)
Chung, Ka Young (School of Pharmacy, Sungkyunkwan University)
Won, Hyung-Sik (Department of Biotechnology, Research Institute (RIBHS) and College of Biomedical and Health Science, Konkuk University)
Seo, Min-Duk (Department of Molecular Science and Technology, Ajou University)
Publication Information
Journal of the Korean Magnetic Resonance Society / v.19, no.1, 2015 , pp. 36-41 More about this Journal
Abstract
The causative agent of shigellosis, Shigella flexneri, is a Gram-negative anaerobic bacterial pathogen that causes one of the most infectious bacterial dysenteries in humans. It originates infection by invading cells of the colonic epithelium using a type III secretion system. Despite S. flexneri is closely linked with the human disease, structural study is very deficient. Here, we have initiated NMR study of SF1002 which is the uncharacterized protein from S. flexneri strain 5a M90T. Based on a series of triple resonance spectra, sequence-specific assignments of the backbone amide resonances of SF1002 could be completed. This NMR study would contribute to the structural genomics of S.flexneri.
Keywords
Shigella flexneri; backbone NMR assignments; structural genomics;
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1 J. Wei, M. B. Goldberg, V. Burland, M. M. Venkatesan, W. Deng, G. Fournier, G. F. Mayhew, G. Plunkett III, D. J. Rose, A. Darling, B. Mau, N. T. perna, S. M. Payne, L. J. Runyen-Janecky, S. Zhou, D. C. Schwartz, F. R. Blattner, Infect. Immun. 71, 2275 (2003)
2 Q. Jin et al., Nucleic Acids Res. 30, 4432 (2002)   DOI
3 M. L. Bennish, B. J. Wojtyniak, Rev. Infect. Dis. 13, S245 (1991)   DOI
4 S. Ashkenaze, I. Levy, V. Kazaronovski, Z. Samra, J. Antimicrb. Chemother. 51, 427 (2003)   DOI
5 P. J. Sansonetti, PLos Med. 3, 1465 (2006)
6 Y.-S. Lee, K.-S. Ryu, Y. Lee, S. Kim, K. W. Lee, H.-S. Won, j. Kor. Magn. Reson. Soc. 15, 137 (2011)   DOI   ScienceOn
7 Y.-S. Lee, H.-S. Ko, K.-S. Ryu, Y.-H. Jeon, H.-S. Won, j. Kor. Magn. Reson. Soc. 14, 117 (2010)   DOI
8 N. T. Onodera, J. Ryu, T. Durbic, C. Nislow, J. M. Archibald, R. Rohde, J. Bacteriol. 194, 3022 (2012)   DOI
9 Y. Shen, F. Delaglio, G. Cornilescu, A. Bax, J. Biomol. NMR 44, 213 (2009)   DOI   ScienceOn
10 D. S. Wishart, B. D. Sykes, J. Biomol. NMR 4, 171 (1994)