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http://dx.doi.org/10.5012/bkcs.2007.28.11.2083

Detection of Avidin Based on Rugate-structured Porous Silicon Interferometer  

Koh, Young-Dae (Department of Chemistry, Chosun University)
Kim, Sung-Jin (Department of Chemistry, Chosun University)
Park, Jae-Hyun (Department of Chemistry, Chosun University)
Park, Cheol-Young (Department of Chemistry, Chosun University)
Cho, Sung-Dong (Department of Chemistry, Chosun University)
Woo, Hee-Gweon (Department of Chemistry, Chonnam National University)
Ko, Young-Chun (Department of Nano-Chemical/Environmental Engineering, Daebul University)
Sohn, Hong-Lae (Department of Chemistry, Chosun University)
Publication Information
Bulletin of the Korean Chemical Society / v.28, no.11, 2007 , pp. 2083-2088 More about this Journal
Abstract
Biosensor based on rugate PSi interferometer for the detection of avidin has been described. Rugate PSi fabricated by applying a computer-generated pseudo-sinusoidal current waveform has been prepared for the application as a label-free biosensor based on porous silicon interferometer. The fabrication, optical characterization, and surface derivatization of a rugate PSi has been described. The method to fabricate biotinderivatized rugate PSi has been investigated. The surface and cross sectional morphology of rugate PSi are obtained with SEM. FT-IR spectroscopy is used to characterize the oxidation and functionalization reaction of rugate PSi sample. Binding of the avidin into the biotin-derivatized rugate PSi induces a change in refractive index. A red-shift of reflectivity by 18 nm in the reflectivity spectrum is observed, when the biotin-modified rugate PSi was exposed to a flow of avidin.
Keywords
Rugate structure; Porous silicon; Biotin; Avidin;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 6  (Related Records In Web of Science)
Times Cited By SCOPUS : 4
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