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http://dx.doi.org/10.22889/KJP.2020.51.3.217

Validation of an HPLC/UV Analysis Method for Cirsimaritin in Cirsium japonicum var. maackii  

Lee, Ju Sung (Department of Plant Science and Technology, Chung-Ang University)
Paje, Leo Adrianne (Department of Plant Science and Technology, Chung-Ang University)
Rodriguez, Joyce P. (Department of Biology, University of San Carlos)
Kang, Ki Sung (College of Korean Medicine, Gachon University)
Hahm, Dae-Hyun (Department of Physiology, College of Medicine, Kyung Hee University)
Shim, Jae Suk (Imsil Herbal Medicine Association)
Choi, Yu-Jin (Imsil Cheese & Food Research Institute)
Lee, Sanghyun (Department of Plant Science and Technology, Chung-Ang University)
Publication Information
Korean Journal of Pharmacognosy / v.51, no.3, 2020 , pp. 217-221 More about this Journal
Abstract
Cirsium japonicum var. maackii (CM) has been used to treat certain medical conditions such as hemorrhage, hepatitis, and hypertension. Cirsimaritin was previously found as the major flavonoid in CM and is said to contribute to its pharmacological effects. There are currently no reports detailing the qualitative and quantitative detection of phytochemical indicators in the aerial parts of CM. Therefore, we developed a method to rapidly identify and quantify cirsimaritin in CM using HPLC/UV, and we optimized and validated this analytical method. The results showed good linearity in the concentration range tested (0.25-0.015 mg/mL, r2 ≥ 0.9999), accuracy (93.9-111.3%), and precision (RSD ≤ 0.59%). The developed method can therefore be used for the rapid evaluation of cirsimaritin in CM.
Keywords
Cirsium japonicum var. maackii; Cirsimaritin; HPLC/UV; Method validation;
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