Browse > Article

Neuroprotective Effects of Ethanol Extract of Ganoderma lucidum L. on murine hippocampal cells  

Lee, Seung Cheol (College of Pharmacy, Keimyung University)
Im, Nam-Kyung (College of Pharmacy, Keimyung University)
Jeong, Hye Young (College of Natural Sciences, Keimyung University)
Choi, Eun Hwa (College of Pharmacy, Keimyung University)
Jeon, Soo Myeong (College of Pharmacy, Keimyung University)
Jeong, Gil-Saeng (College of Pharmacy, Keimyung University)
Publication Information
Korean Journal of Pharmacognosy / v.45, no.2, 2014 , pp. 161-167 More about this Journal
Abstract
Ganoderma lucidum L. (GL) is a traditional oriental medicine that has been widely used as anti-inflammatory, antitumor and anti-oxidant in Korea and other Asian countries. In this study, we investigated the ethanol extract of GL has neuroprotective effects in murine hippocampal HT22 cells. GL ethanol extract has the potent neuroprotective effects on glutamate-intoxicated cells by inducing the expression of heme oxygenase (HO)-1 in HT22 cells. GL ethanol extract increased JNK phosphorylation. Obviously, When we treated the GL extract with c-Jun N-terminal kinase (JNK) inhibitor (SP600125), HO-1 expression was reduced. Moreover, we found that GL treatment caused the nuclear accumulation of Nrf2. In conclusion, the ethanol extract of GL significantly protects glutamate-induced oxidative damage by induction of HO-1 via Nrf2, JNK pathway in mouse hippocampal HT22. These results suggest that GL ethanol extract would be a good source for taking active compounds and may be a potential pharmaceutical products for brain disorder induced by neuronal damage and oxidative stress.
Keywords
Ganoderma lucidum L.; Anti-oxidant; Neuroprotective; HT22; Ethanol extract; Heme oxygenase-1;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Sliva, D., Labarrere, C., Slivova, V., Sedlak, M., Lloyd, Jr. F. P. and Ho, N. W. (2002) Ganoderma lucidum suppresses motility of highly invasive breast and prostate cancer cells. Biochem. Biophys. Res. Commun. 298: 602-612.
2 Stanciu, M., Wang, Y., Kentor, R., Burke, N., Watkins, S., Kress, G., Reynolds, I., Klann, E., Angiolieri, M. R., Johnson, J. W. and DeFranco, D. B. (2000) Persistent activation of ERK contributes to glutamate-induced oxidative toxicity in a neuronal cell line and primary cortical neuron cultures. J. Biol. Chem. 275: 12200-12206.   DOI   ScienceOn
3 Chen, C. B., Ng, J. K., Choo, P. H., Wu, W. and Porter, A. G. (2009) Mammalian sterile 20-like kinase 3 (MST3) mediates oxidative-stress-induced cell death by modulating JNK activation. Biosci. Rep. 29: 405-415.   DOI   ScienceOn
4 Gursoy, E., Cardounel, A. and Kalimi, M. (2001) The environmental estrogenic compound bisphenol A exerts estrogenic effects on mouse hippocampal (HT-22) cells: neuroprotection against glutamate and amyloid beta protein toxicity. Neurochem. Int. 38: 181-186.   DOI   ScienceOn
5 Jeong, G.S., Li, B., Lee, D. S., Choi, H. G. and Kim, Y. C. (2010) Neuroprotective effects of the extract of Zingiberis Rhizoma. Kor. J. Pharmacogn. 41: 190-195.
6 Choi, H. G., Lee, D. S., Li, B., Jun, K. Y., Jeong, G. S. and Kim, Y. C. (2011) Neroprotective effect of the water-insoluble fraction of root barks of Dictamnus dasycarpus 70% ethanol extract on glutamate-induced oxidative damage in mouse hippocampal HT22 cells. Kor. J. Pharmacogn. 42: 175-181.
7 Fukui, M., Song, J. H., Choi, J., Choi, H. J. and Zhu, B. T. (2009) Mechanism of glutamate-induced neurotoxicity in HT22 mouse hippocampal cells. Eur. J. Pharmacol. 617: 1-11.   DOI   ScienceOn
8 Ye, S., Chen, M., Jiang, Y., Chen, M., Zhou, T., Wang, Y., Hou, Z. and Ren, L. (2014) Polyhydroxylated fullerene attenuates oxidative stress-induced apoptosis via a fortifying Nrf2-regulated cellular antioxidant defence system. Int. J. Nanomed. 9: 2073-2087.
9 Tan, M., Ouyang, Y., Jin, M., Chen, M., Liu, P., Chao, X., Chen, Z., Chen, X., Ramassamy, C., Gao, Y. and Pi, R. (2013) Downregulation of Nrf2/HO-1 pathway and activation of JNK/c-Jun pathway are involved in homocysteic acidinduced cytotoxicity in HT-22 cells. Toxicol. Lett. 223: 1-8.   DOI   ScienceOn
10 Itoh, T., Koketsu, M., Yokota, N., Touho, S., Ando, M. and Tsukamasa, Y. (2014) Reduced scytonemin isolated from Nostoc commune suppresses LPS/IFN $\gamma$-induced NO production in murine macrophage RAW264 cells by inducing hemeoxygenase-1 expression via the Nrf2/ARE pathway. Food Chem. Toxicol. 69: 330-338.   DOI   ScienceOn
11 Coyle, J. T. and Puttfarcken, P. (1993) Oxidative stress, glutamate, and neurodegenerative disorders. Science 262: 689-695.   DOI   ScienceOn
12 Mizushina, Y., Takahashi, N., Hanashima, L., Koshino, H., Esumi, Y., Uzawa, J., Sugawara, F. and Sakaguchi, K. (1999) Lucidenic acid O and lactone, new terpene inhibitors of eukaryotic DNA polymerases from a basidiomycete, Ganoderma lucidum. Bioorg. Med. Chem. 9: 2047-2052.
13 Hasnat, A., Pervin, M. and Lim, B. O. (2013) Acetylcholinesterase inhibition and in vitro and in vivo antioxidant activities of Ganoderma lucidum grown on germinated brown rice. Molecules 6: 6663-6678.
14 Gao, J. J., Min, B. S., Ahn, E. M., Nakamura, N., Lee, H. K. and Hattori, M. (2002) New triterpene aldehydes, lucialdehydes A-C, from Ganoderma lucidum and their cytotoxicity against murine and human tumor cells. Chem. Pharm. Bull. 50: 837-840.   DOI   ScienceOn
15 Jin, M. L., Park, S. Y., Kim, Y. H., Oh, J. I., Lee, S. J. and Park, G. (2014) The neuroprotective effects of cordycepin inhibit glutamate-induced oxidative and ER stress-associated apoptosis in hippocampal HT22 cells. Neurotoxicology 41: 102-111.   DOI
16 Butterfield, D. A., Hensley, K., Cole, P., Subramaniam R., Aksenov M., Aksenova M., Bummer P. M., Haley B. E. and Carney J. M. (1997) Oxidatively induced structural alteration of glutamine synthetase assessed by analysis of spin label incorporation kinetics: relevance to Alzheimer's disease. J. Neurochem. 68: 2451-2457.
17 Rssler, O. G., Bauer, I., Chung, H. Y. and Thiel, G. (2004) Glutamate-induced cell death of immortalized murine hippocampal neurons: neuroprotective activity of heme oxygenase-1, heat shock protein 70, and sodium selenite. Neurosci. Lett. 362: 253-257.   DOI   ScienceOn
18 Cheng, P. G., Phan, C. W., Sabaratnam, V., Abdullah, N., Abdulla, M. A. and Kuppusamy, U. R. (2013) Polysaccharides-Rich extract of Ganoderma lucidum (M.A Curtis:Fr.) P. Karst accelerates wound healing in streptozotocin-induced diabetic rats. Evid.-based Complement Altern. Med. 24: 41-50.
19 Yun, S. S., Kim, S. H., Sa, J. H., Jin, C. B., Lim, C. J. and Park, E. H. (2004) Anti-angiogenic and inhibitory activity on inducible nitric oxide production of the mushroom Ganoderma lucidum. J. Ethnopharmacol. 90: 17-20.   DOI   ScienceOn
20 Zhang, J., Tang, Q., Zimmerman-Kordmann, M., Reutter, W. and Fan, H. (2002) Activation of B lymphocytes by GLIS, a bioactive proteoglycan from Ganoderma lucidum. Life Sci. 71: 623-638.   DOI   ScienceOn
21 Zhang, H. N., He, J. H., Yuan, L. and Lin, Z. B. (2003) In vitro and in vivo protective effect of Ganoderma lucidum polysaccharides on alloxan-induced pancreatic islets damage. Life Sci. 73: 2307-2319.   DOI   ScienceOn
22 Wang, S. Q., Li, X. J., Zhou, S., Sun, D. X., Wang, H., Cheng, P. F., Ma, X. R., Liu, L., Liu, J. X., Wang, F. F., Liang, Y. F. and Wu, J. M. (2013) Intervention effects of Ganoderma lucidum spores on epileptiform discharge hippocampal neurons and expression of neurotrophin-4 and N-cadherin. PLoS One 8: 15-22.
23 Bao, X. F, Zhen, Y., Ruan, L. and Fang, J. N. (2002) Purification, characterization, and modification of T lymphocytestimulating polysaccharide from spores of Ganoderma lucidum. Chem. Pharm. Bull. 50: 623-629.   DOI   ScienceOn
24 Eo, S. K., Kim, Y. S., Lee, C. K. and Han, S. S. (2000) Possible mode of antiviral activity of acidic protein bound polysaccharide isolated from Ganoderma lucidum on herpes simplex viruses. J. Ethnopharmacol. 72: 475-481.   DOI   ScienceOn
25 Oh, K. W., Lee, C. K., Kim, Y. S., Eo, S. K. and Han, S. S. (2000) Antiherpetic activities of acidic protein bound polysacchride isolated from Ganoderma lucidum alone and in combinations with acyclovir and vidarabine. J. Ethnopharmacol. 72: 221-227.   DOI   ScienceOn
26 Berger, A., Rein, D., Kratky, E., Monnard, I., Hajjaj, H., Meirim, I., Piguet-Welsch, C., Hauser, J., Mace, K. and Niederberger, P. (2004) Cholesterol-lowering properties of Ganoderma lucidum in vitro, ex vivo, and in hamsters and minipigs. Lipids Health Dis. 3: 311-323.
27 생약학교재편찬위원회 (2007) 생약학, 649-652, 동명사, 서울.
28 한국 약용식물학 연구회 (2001) 종합약용식물학, 86, 학창사, 서울.