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HPLC Analysis of Caffeoylquinic Acids in the Extract of Cacalia firma and Peroxynitrite Scavenging Effect  

Park, Hee-Juhn (Department of Pharmaceutical Engineering, Sangji University, Sangji University)
Nugroho, Agung (Department of Pharmaceutical Engineering, Sangji University, Sangji University)
Lee, Jin-Ha (Department of Food Science & Biotechnology, Kangwon National University)
Kim, Jong-Dae (Department of Food Science & Biotechnology, Kangwon National University)
Kim, Won-Bae (Highland Agriculture Research Center, Rural Development Administration)
Lee, Kang-Ro (College of Pharmacy, SungKyunKwan University)
Choi, Jae-Sue (Devision of Food Science and Biotechnology, Pukyong National University)
Publication Information
Korean Journal of Pharmacognosy / v.40, no.4, 2009 , pp. 365-369 More about this Journal
Abstract
Six caffeoylquinic acids of Cacalia firma (Komarov) Nakai (Compositae) leaves were identified using standard compounds by HPLC. Each content of those compounds in dried weight was determined as follows: 3,4-di-O-caffeoylquinic acid (1.44${\pm}$0.04 mg/g of dried weight), 3,5-di-O-caffeoyl-muce-quinic acid (2.47${\pm}$0.12 mg/g), 3,5-di-O-caffeoylquinic acid (3.74${\pm}$0.24 mg/g), 5-caffeoylquinic acid (chlorogenic acid, 5.20${\pm}$0.09 mg/g), 3-caffeoylquinic acid (1.35${\pm}$0.01 mg/g) and 3-Op-coumaroylquinic acid (3.84${\pm}$0.25 mg/g). The total content of six caffeoylquinic acids in the plant material was calculated as 18.05${\pm}$0.69 mg/g while the percentage of the six compounds in the MeOH extract was calculated as 30.85${\pm}$1.18%. The $IC_{50}$ value of the MeOH extract scavenging peroxynitrite ($ONOO^-$) was shown as 3.22${\pm}$0.57 ${\mu}g$/ml.
Keywords
Cacalia firma; Compositae; caffeoylquinic acid; peroxynitrite; HPLC;
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Times Cited By KSCI : 5  (Citation Analysis)
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