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http://dx.doi.org/10.7778/jpkm.2015.29.2.026

Effect of Geumsuyukgunjeon on Airway Mucus Secretion and Mucin Production  

Kim, Eun Jin (Department of Pediatrics, College of Oriental Medicine, Dongguk University)
Min, Sang Yeon (Department of Pediatrics, College of Oriental Medicine, Dongguk University)
Kim, Jang Hyun (Department of Pediatrics, College of Oriental Medicine, Dongguk University)
Publication Information
The Journal of Pediatrics of Korean Medicine / v.29, no.2, 2015 , pp. 26-36 More about this Journal
Abstract
Objectives In this study, effect of Geumsuyukgunjeon (GYJ) on the increase in airway epithelial mucosubstances of rats with acute bronchitis and EGF-induced MUC5AC mucin production from human airway epithelial cells were investigated. Materials and Methods Hypersecretion of airway mucus was induced by exposure of rats to SO2 during 3 weeks. Effect of orally-administered GYJ during 2 weeks on increase in airway epithelial mucosubstances from tracheal goblet cells of rats was assesed using histopathological analysis after staining the epithelial tissue with PAS-alcian blue. Possible cytotoxicity of GYJ was assessed by examining the potential damage of kidney and liver functions by measuring serum GOT/GPT activities and serum BUN and creatinine concentrations of rats and the body weight gain during experiment, after administering GYJ orally. Effect of GYJ on EGF-induced MUC5AC mucin production from human airway epithelial cells (A549) was investigated. Confluent A549 cells were pretreated for 30 min in the presence of GYJ and treated with EGF (25 ng/ml) for 24 hrs, to assess the effect of GYJ on EGF-induced MUC5AC mucin production using enzyme-linked immunosorbent assay (ELISA). Results (1) GYJ decreased the amount of intraepithelial mucosubstances of trachea of rats. (2) GYJ did not show kidney and liver toxicities and did not affect body weight gain of rats during experiment. (3) GYJ significantly inhibited EGF-induced MUC5AC mucin production from A549 cells. Conclusions The result from the present study suggests that GYJ might control both the mucus hypersecretion in vivo and do not show in vivo toxicity to liver and kidney functions after oral administration and the production of pulmonary mucin.
Keywords
Geumsuyukgunjeon (GYJ); Pulmonary mucus; MUC5AC;
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1 Kim KB, Kim DG, Kim YH, Kim JH, Min SY, Park EJ, Baek JH, Yu SA, Lee SY, Lee JY, Lee HJ, Chang GT, Chai JW, Han YJ, Han JK. Hanbangsoacheongsonyeonuihak. Seoul: Ui Sung Dang Publishing Co. 2010:339-40, 357.
2 Ali M, Lillehoj EP, Park Y, Kyo Y, Kim KC. Analysis of the protome of human airway epithelial secretions. Proteome Sci. 2011;9:4.   DOI
3 Sim YS. Clinical respiratiry system. Seoul: Iljogak. 1990:6-8.
4 Lee CJ, Ko KH. Pathophysiologic characteristics of airway mucus-hypersecretory diseases and experimental models. Biomol Ther. 1998;6(1):1-8.
5 Rogers DF. Airway mucus hypersecretion in asthma: an undervalued pathpology. Curr Opin Pharmacol. 2004;4(3):241-50.   DOI
6 Rogers DF, Barnes PJ. Treatment of airway mucus hypersecretion. Annal Med. 2006;38(2):116-25.   DOI
7 Hong WS, Kim YN. Gukyeok kangeokzeonseo. Seoul: Iljungsa. 1992:34.
8 Yan YM. Kangeokzeonseo. Seoul: Hanmiuihak. 2006:1920.
9 Lee HG, Jung ki, Jung HJ, Kim JD. Donguipyegeneagwahak. Seoul: Gukjingihoek. 2004: 114, 168.
10 Lee SY, Park DI. Effects of Gyurnsuyukgunjun on the injured tracheal tissue induced $SO_{2}$ in rats. Korean J Orient Med. 1996;2(1):226-35.
11 Kam CW, Park DI. Experimental study of the effects of Gyumsuyukgunjun on the pulmonary injury caused by exposute of cigarette smoke in rat. Korea Institute of Sci Technol Inf. 1997;3(1):261-78.
12 Kim CS, Choi HY, Kim JD. Effects of Kumsooyukkun-jeon on asthma induced by ovalbumin in mice. J Korean Med. 2002;23(3):104-18.
13 Song JJ, Park YC, Kim BT, Kim SH. Effect of Kamkeumsooyukkunjeon on allergy and lung damage. Korean Soc Orient Med Pathol. 2002;14(2):70-9.
14 Hwang DY. Bangyakhappyeon. Seoul: Yonglimsa. 2002:148-9.
15 Pon DJ, Van Staden CJ, Boulet L, Roger IW. Hyperplastic effects of aerosolized sodium metasulfite on rat airway mucus-secretory epithelial cells. Can J Physiol Pharmacol. 1994;72(9):1025-30.   DOI
16 Lee, CJ. Effects of poly-L-arginine on the mucin release from airway goblet cells of hamster and on the mucosubstances of airway goblet cells of rat. J Appl Pharmacol. 2001;9(4):263-9.
17 St George JA, Cranz DL, Zicker S, Etchison JR, Dungworth DL, Plopper CG. An immunohistochemical characterization of Rhesus monkey respiratoty secretions using monoclonal antibodies. Am Rev Resp Dis. 1985;132:556-63.
18 Shao MXG, Ueki IF, Nadel JA. Tumor necrosis factor alpha converting enzyme mediates MUC5AC mucin expression in cultured human airway epithelial cells. Proc Natl Acad Sci USA. 2003;100:11618-23.   DOI
19 Song KS, Lee WJ, Chung KC, Koo JS, Yang EJ, Choi JY, Yoon JH. Interleukin-$1{\beta}$ and tumor necrosis factor-alpha Induce MUC5AC overexpression through a mechanism involving ERK/p38 mitogen-activated protein kinases-MSK1-CREB activation in human airway epithelial cells. J Biol Chem. 2003;278:23243-50.   DOI
20 The Korean Acedemy of Pediatric Allergy and Respiratory Disease. Pediatrics allergy immunology pulmonology. Seoul: Goonjachulpansa. 2005:267.
21 Kang YI, Jang GM. Silyongjongeigwahak. Sanghaigwahakgisulchulpansa. 1995:455-61.
22 The Korean Acedemy of Asthma, Allergy and clinical immunology. Asthma and allergy. Seoul: Gunjachulpansa. 2002:73-6.
23 Korea institute for health and social affairs. Patients survey in 2013[Internet]. Available from: http://stat.mw.go.kr/front/statData/publicationView.jsp?menuId=45&bbsSeq=11&nttSeq=21200&searchKey=&searchWord=&nPage=1
24 Health insurance review and assessment service. Survey of diseases and service: acute bronchitis(J20), chronic bronchitis(J242)[Internet]. Available from: http://www.hira.or.kr/rd/dissdic/infoSickList.do? pgmid=HIRAA020044020100
25 Jany B, Gallup M, Tsuda T, Basbaum CB. Mucin gene expression in rat airways following infectin and irritation. Biocherm Biophys Res Comm. 1991:181.
26 Culpiitt SV, Rogers DF, Traves SL, Barnes PJ, Donnelly LE. Sputum matrix metalloprotease: comparison between chronic obstructive pulmonary disease and asthma. Respir Med. 2005;99(6):703-10.   DOI
27 Kim DJ, Kim KU, Namgung EK. Measurement of mucin amounts using RTO3 in patients with chronic airway disease. Tuberc Respir Dis. 1999;47(6):792-3.
28 Zuhji Alimam M. Muc-5/5ac mucin messenger RNA and protein expression is a marker of goblet cell metaplasia in murine airways. Am J Respir Cell Mol Biol. 2000;22:253-60.   DOI
29 Hoorens PR, Rinaldi M, Li RW, Goddeeris B, Claerebout E, Wercruysse J. Genome wide analysis of the bovine mucin genes and their gastrointestinal transcription profile. BMC Genomics. 2011;12:140.   DOI
30 Rousseau K, Kirkham S, McKane S, Newton R, Clegg P, Thornton DJ. Muc5b and muc5a are the major oligomeric mucins in equine airway mucus. Am J Physiol Lung Cell Mol Physiol. 2007;292:1396-404.   DOI
31 Sin MG. Ilmsangyeongyongbonchuhak. Seoul: Namsandang. 1988:176-7, 219, 221-2, 250-1, 254-5, 380-1, 556-7.
32 Shim JJ, Dabbagh K. Suplatast tosilate inhibits goblet- cell metaplasia of airway epithelium in sensitized mice. J Allergy Clin Immunol. 2000;105:739-45.   DOI
33 Jung HJ, Jung SK, Lee HG. Review of literatures about sputum on west-oriental medicine. J Orient Chronic Dis. 1995;1(1):51-62.
34 Lee SI, An DG, Sin MG. Hanyakilmsangyeongyong. Seoul: Sungbosa. 1986:355-6, 395.
35 Bea BC. Ilmsangbangjahak. Seoul: Sungbosa. 1995: 414.