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http://dx.doi.org/10.14406/acu.2013.034

The Anti-Migratory Effect of Cirsium japonicum Pharmacopuncture in C6 Glioma Cell  

Park, Juyeon (Department of Acupoint, College of Korean Medicine, Dongguk University)
Lee, Kangpa (Division of Bio Science, College of Science and Technology, Dongguk University)
Chang, Haeryong (Division of Bio Science, College of Science and Technology, Dongguk University)
Moon, Jinyoung (Department of Acupoint, College of Korean Medicine, Dongguk University)
Publication Information
Korean Journal of Acupuncture / v.30, no.4, 2013 , pp. 212-219 More about this Journal
Abstract
Objectives : Cirsium japonicum is a traditional Korean medicine that has been used in the treatment of inflammatory diseases such as appendicitis, hepatitis, pulmonary abscess and tumor. The aim of study was to elucidate anti-migratory activity of CJP(Cirsium japonicum pharmacopuncture) through regulation of inflammatory mediators in C6 glioma cell. Methods : Nitric oxide(NO) production was determined by using nitrite assay. The cell migration was analyzed by wound-healing assay and Boyden chamber assay. The expression levels of iNOS, and protein kinase C(PKC)-${\alpha}$ were measured by western blotting assay. Results : CJP showed a significant decrease on NO production. Moreover, glioma cell migration was effectively suppressed by CJP. Furthermore, CJP inhibited the expressions of iNOS and PKC-${\alpha}$ in C6 glioma cells. Conclusions : These results suggest that CJP inhibits glioma cell migration and iNOS expression through regulation of PKC-${\alpha}$. Therefore, it is expected that CJP could be an effective agents for blocking malignant progression of glioma.
Keywords
cirsium japonicum; migration; iNOS; PKC-${\alpha}$; C6 glioma cells;
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