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http://dx.doi.org/10.18325/jkmr.2021.31.1.59

Genotoxicity Study of ChondroT  

Kim, Sun-Gil (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University)
Kim, Joo Il (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University)
Kim, Ji-Hoon (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University)
Yoon, Chan Suk (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University)
Jeong, Ji-Won (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University)
Na, Chang-Su (Department of Meridian and Acupoint, College of Korean Medicine, Dongshin University)
Kim, Seon-Jong (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University)
Publication Information
Journal of Korean Medicine Rehabilitation / v.31, no.1, 2021 , pp. 59-79 More about this Journal
Abstract
Objectives This study was performed to observe the genotoxic effect of the ChondroT. Methods To evaluate the genotoxicity of ChondroT, an experiment of bacterial reverse mutation test, in vitro mammalian chromosomal aberration test and mammalian erythrocyte micronucleus test in mouse was conducted. Results TA98, TA100 and TA1537 strains in the absence of metabolic activation system (S9 mix), the number of revertant colonies being greater than 2-fold of the respective negative control value. Both in -S9 mix and +S9 mix, the frequencies of aberration cells with structural aberration and numerical aberrations of chromosome were less than 5%. There was no increase of polychromatic erythrocyte with one or more micronuclei at any dose of test substance compared to the negative control group (p<0.05). Conclusions In TA98, TA100 and TA1537 strains in the absence of metabolic activation system (S9 mix), the number of revertant colonies was greater than 2-fold of the respective negative control value, showing positive results. ChondroT was considered to be non-clastogenic to Chinese hamster lung (CHL/IU) cells under the present experimental condition. and ChondroT was determined not to induce an increased frequency of micronuclei in the bone marrow cells of male ICR mice under the present experimental condition.
Keywords
ChondroT; Mutagenicity tests; Chromosome aberrations; Micronucleus tests;
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