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http://dx.doi.org/10.9799/ksfan.2012.25.4.863

Gene Profile of Mesenchymal Stem Cell Induced by SAC or Hydrogen Peroxide (H2O2)  

Park, Ran-Sook (Dept. of Foods & Nutrition, SoongEui Women's College)
Publication Information
The Korean Journal of Food And Nutrition / v.25, no.4, 2012 , pp. 863-870 More about this Journal
Abstract
Though hydrogen peroxide ($H_2O_2$) causes a deleterious effect to cells with its reactive oxygen species resulting in cell death, S-allyl cysteine (SAC, a bioactive organosulfur compound of aged garlic extract) has been known to have a cytoprotective effect. Few reported profiles of gene expression of $H_2O_2$ and SAC treated human cord blood derived mesenchymal stem cells (MSC). This study revealed changes in the profile of twenty-one genes grouped by oxidative stress, antioxidant, cell death, anti-apoptosis and anti-aging by quantitative real time PCR. A concentration of $100{\mu}M$ of SAC or $50{\mu}M$ of $H_2O_2$ was applied to MSC which show moderate growth and apoptosis pattern. $H_2O_2$ treatment enhanced expression of eleven genes out of twenty-one genes compared with that of control group, on the contrary SAC suppressed expression of eighteen genes out of twenty-one genes except C ros oncogene. SAC decreased expression of oxidative stress genes such as SOD1, CAT and GPX. These results seemed consistent with reports which elucidated over-expression of NF-${\kappa}$B by $H_2O_2$, and suppression of it by SAC. This study will confer basic information for further experiments regarding the effects of SAC on gene levels.
Keywords
S-allyl cysteine; hydrogen peroxide; gene expression; real time PCR;
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