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http://dx.doi.org/10.11626/KJEB.2021.39.3.259

Quantitative analysis of viral hemorrhagic septicemia virus in tissues of infected olive flounder(Paralichthys olivaceus)  

Jang, Jin Hyeon (Aquatic Disease Control Division, National Fishery Products Quality Management Services)
Hwang, Seong Don (Aquaculture Industry Division, East Sea Fisheries Research Institute)
Jung, Ji Min (Aquatic Disease Control Division, National Fishery Products Quality Management Services)
Kwon, Mun-Gyoung (Aquatic Disease Control Division, National Fishery Products Quality Management Services)
Hwang, Jee Youn (Aquatic Disease Control Division, National Fishery Products Quality Management Services)
Publication Information
Korean Journal of Environmental Biology / v.39, no.3, 2021 , pp. 259-265 More about this Journal
Abstract
A diagnostic test for viral hemorrhagic septicemia virus (VHSV), which infects more than 80 species of freshwater and marine fish at home and abroad, causing mass mortality, was conducted to provide quantitative data on the amount of virus expression in various tissues of flounder in chronological order. The tissues were collected in chronological order after the intraperitoneal injection of 3.0E+07 tissue culture infective dose50 (TCID50) per 0.1mL per fish of VHSV to randomly selected flounder. As a result of relative quantification through real-time PCR, the highest levels of virus expression were found in the spleen, kidney, gill, and liver on day 5. This study proved that the spleen was an appropriate site for the final diagnosis of VHSV in the early stages of infection and will provide important information for the diagnosis of legal infectious diseases in Korea.
Keywords
real-time PCR; viral hemorrhagic septicemia virus (VHSV); olive flounder; diagnosis;
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