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http://dx.doi.org/10.12925/jkocs.2022.39.5.623

Design Optimization of Hydrated Liquid Crystalline Vesicles Containing a High Content of Ceramide Using DOE  

Shin, Juyeong (Division of Chemistry & Cosmetics, Dongduk Women's University)
Jin, Byung-Suk (Division of Chemistry & Cosmetics, Dongduk Women's University)
Publication Information
Journal of the Korean Applied Science and Technology / v.39, no.5, 2022 , pp. 623-631 More about this Journal
Abstract
Using the design of experiment (DOE), factors affecting the particle size of hydrated liquid crystalline vesicles containing a high content of ceramide were analyzed and the mixture composition was optimized. Manufacturing temperature, amount of ethanol, and ultrasonic time were selected as the main variables affecting the droplet size of the vesicles, and the effect of these variables on the droplet size was examined through the signal to noise (S/N) ratios of Taguchi method and ANOVA analysis. In addition, mixture composition experiments of three lipid components constituting the vesicle membrane, hydrogenated phosphatidyl choline (HPC), cholesterol (Chol), and ceramide (Cer), were performed according to the simplex central design matrix of the mixture. Regression analysis was conducted with the experimental data to obtain a model equation, and the optimal mixing composition of the three lipid components to minimize the vesicle droplet size was determined as HPC (0.6), Chol (0.1), and Cer (0.3).
Keywords
Ceramide; Hydrated liquid crystalline vesicle; Design of experiment; Taguchi method; ANOVA analysis;
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