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http://dx.doi.org/10.15429/jkomor.2022.22.1.1

Hesperidin and Hesperetin Protect against Oxidative Stress on Hepatic Toxicity in Rats  

Kim, Ji Hyun (Department of Food Science and Nutrition, Gyeongsang National University)
Li, Li (Department of Food Science and Nutrition, Pusan National University)
Kim, Mi Suk (Department of Food Science, Gyeongsang National University)
Cho, Eun Ju (Department of Food Science and Nutrition, Pusan National University)
Kim, Hyun Young (Department of Food Science and Nutrition, Gyeongsang National University)
Choi, Jine Shang (Department of Food Science and Technology, Gyeongsang National University)
Publication Information
Journal of Korean Medicine for Obesity Research / v.22, no.1, 2022 , pp. 1-10 More about this Journal
Abstract
Objectives: To investigate the protective effect of hesperidin and hesperetin against oxidative stress in 2,2'-azobis (2-aminopropane) dihydrochloride (AAPH)-induced liver toxicity in rats. Methods: Hesperidin or hesperetin (200 mg/kg/day, respectively) was orally administered for 7 days once daily in rats. Subsequently, AAPH (50 mg/kg/day) was administered intraperitoneally. Lipid peroxidation, nitric oxide production, catalase activity, and protein expressions of nuclear factor-kappa B (NF-κB) and inducible nitric oxide synthase (iNOS) in the liver tissues were measured. Results: Administration of hesperidin and hesperetin significantly decreased serum aspartate transaminase and alanine transaminase levels in AAPH-induced oxidative stress liver tissues compared with control group. Lipid peroxidation and nitric oxide (NO) production were also significantly reduced by hesperidin and hesperetin in AAPH-induced oxidative stress liver tissues. In particular, lipid peroxidation levels of hesperetin-administered group significantly decreased to 5.02 nmole/mg protein in oxidative stress rats. Hesperidin and hesperetin significantly increased antioxidant activity, such as that of catalase. Furthermore, administration of hesperidin and hesperetin substantially down-regulated the expression of NF-κB and iNOS in liver tissues. Administration of hesperidin reduced NO levels and iNOS expression more than in the hesperetin-administered group. Conclusions: Administration of hesperidin and hesperetin led to a reduction in AAPH-induced liver toxicity by regulating oxidative stress.
Keywords
Oxidative stress; Hesperidin; Hesperetin; Catalase; Nitric oxide;
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