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http://dx.doi.org/10.14374/HFS.2018.26.2.103

Antioxidant Effect of Rubi Fructus on TM4 Sertoli Cells  

Kim, Young Joo (Department of Prescriptionology, College of Korean Medicine, Graduate School, Kyung Hee University)
Chang, Mun Seog (Department of Prescriptionology, College of Korean Medicine, Graduate School, Kyung Hee University)
Park, Seong Kyu (Department of Prescriptionology, College of Korean Medicine, Graduate School, Kyung Hee University)
Publication Information
Herbal Formula Science / v.26, no.2, 2018 , pp. 103-111 More about this Journal
Abstract
Objectives : The purpose of this study was to examine the antioxidant effects of the extract of Rubi Fructus on TM4 Sertoli cells. Methods : The extract was studied for diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity and cell viability assays on Sertoli cells. In addition, hydrogen peroxide-induced oxidative stress on Sertoli cells were examined by MTT assay. The antioxidant enzyme of Cu/Zn SOD, Mn SOD, catalase protein expression on Sertoli cells were also measured. Results : The results showed that the extract scavenged DPPH radical dose-dependent manner. The extract showed no cytotoxicity at concentration of 1, 5, 10, 50, $100{\mu}g/ml$. The hydrogen peroxide-induced cytotoxicity of Sertoli cells was protected to 88.3% by the extract at concentration of $100{\mu}g/ml$. Cu/Zn SOD and Mn SOD protein expression were significantly increased on Sertoli cells, but catalase protein expression was not significantly changed. Conclusions : In conclusion, the extract of Rubi Fructus has antioxidant effects on Sertoli cells and protect male reproductive system against oxidative stress.
Keywords
Rubi Fructus; Sertoli cells; diphenyl-picryl-hydrazyl (DPPH); MTT assay; hydrogen peroxide; Cu/Zn SOD; Mn SOD; catalase;
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