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http://dx.doi.org/10.5407/jksv.2021.19.1.074

Oscillatory behavior of microglial cells  

Park, Eunyoung (Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology)
Cho, Youngbin (Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology)
Ko, Ung Hyun (Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology)
Park, Jin-Sung (Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology)
Shin, Jennifer H. (Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology)
Publication Information
Journal of the Korean Society of Visualization / v.19, no.1, 2021 , pp. 74-80 More about this Journal
Abstract
Cells regulate their shapes and motility by sensing the cues from the internal and external microenvironment. Under different circumstances, microglia, the brain resident immune cells, undergo dynamic phenotypic changes, one of which is a remarkable periodic oscillatory migration in vitro. However, very little is known about the kinematic and dynamic perspectives of this oscillatory behavior. In this study, we tracked the changes in cell morphology and nuclear displacement, and visualized the forces using traction force microscopy (TFM). By correlation analyses, we confirmed that the lamellipodia formation preceded the nuclear translocation. Moreover, traction, developed following lamellipodia formation, was found to be localized and fluctuated at two ends of the oscillating cells. Taken together, our results imply that oscillatory microglial cells feature a viscoelastic migration, which will contribute to the field of cell mechanics.
Keywords
Microglia; Cellular oscillatory migration; Traction force; Viscoelasticity; Correlation analysis;
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