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http://dx.doi.org/10.5407/JKSV.2013.11.1.016

Measurement of Diffusion Coefficient in Cell-Laden Agarose Gel with Different Cell Concentrations  

Lee, Byung Ryong (한국산업기술대학교 대학원 기계공학과)
Jin, Songwan (한국산업기술대학교 기계공학과)
Publication Information
Journal of the Korean Society of Visualization / v.11, no.1, 2013 , pp. 16-21 More about this Journal
Abstract
In this study, diffusion coefficients of 20 kDa FITC-dextran in 2% agarose gel with different cell concentrations were measured using fiberoptic-based fluorescence recovery after photobleaching technique. As increasing cell concentration suspended in agarose gel, the diffusion coefficients were decreased. The diffusion coefficient of agarose gel which contains $10{\times}10^6$ cells/ml was decreased to 11% that of in agarose gel without cells. The distribution of fluorescence dye in 3D scaffold was also simulated. The simulation result shows that the diffusion coefficient is more significant factor than the scaffold structure.
Keywords
Diffusion Coefficient; Fiberoptic-base FRAP; Cell-laden Agarose Gel; Scaffold;
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