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http://dx.doi.org/10.5407/JKSV.2011.9.1.029

Visualization and Velocity Measurement of Migrating Cells in Microchannels with Various Width  

Lee, Seung-Youl (한국산업기술대학교 기계공학과)
Kim, Myung-Jun (한국산업기술대학교 기계공학과)
Chae, Sang-Min (한국산업기술대학교 기계공학과)
Jin, Song-Wan (한국산업기술대학교 기계공학과)
Publication Information
Journal of the Korean Society of Visualization / v.9, no.1, 2011 , pp. 29-35 More about this Journal
Abstract
In this paper, we applied various microfluidic chambers to visualize cell migration and measured the migration velocity in microchannels. Migration speed of B16 cell in a large channel was similar with that of wound healing experiment. However, the speed decreased gradually as the cell move inside of the channel. It is expected that this phenomenon is due to the shortage of oxygen or nutrition, whichare essential for cell growth. In the case of cell in the small channel whose size is smaller than the cell, its speed was slower than that in a larger channel.
Keywords
Cell migration; Microchannel; Wound healing experiment; PDMS;
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