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Effects of Salinity Stress on the Composition of Free Amino Acids of the Pacific abalone Haliotis discus discus  

Yang, Hyun-Sung (School of Applied Marine Science, College of Ocean Science, Cheju National University)
Park, Kyung-Il (Department of Aquatic Life Medicine, College of Ocean Science, Kunsan National University)
Hong, Choong-Hee (Marine Affairs and Fisheries Policy Division)
Choi, Kwang-Sik (School of Applied Marine Science, College of Ocean Science, Cheju National University)
Publication Information
Journal of Aquaculture / v.21, no.4, 2008 , pp. 218-225 More about this Journal
Abstract
Changes in 13 free amino acids (FAA) of Haliotis discus discus exposed to various salinities were investigated using a high performance liquid chromatography (HPLC). Taurine, glycine and alanine are three major contributors to the total FAA in the gill tissues of H. discus discus. Concentration of taurine was 114 imol/g dry tissue weights accounting for 76.64% of total FAA in H. discus discus. Levels of most FAAs in H. discus discus exposed to low and high salinities for 24 h decreased dramatically. Taurine concentration was slightly increased in the samples exposed to 20 psu and 25 psu for 48 h, and greatly increased after 120 h exposure. After 48 hrs exposure to 20 psu, 25 psu, 30 psu and 40 psu, methionine in H. discus discus was not detected; no methionine was detected in the sample exposed to 20 psu, 25 psu, 30 psu and 40 psu after 120 h of exposure. Taurine:glycine ratio increased depending upon hyper-hypoosmotic condition as well as period of osmotic stress. These data indicate that taurine, glycine and methionine play important role in regulating osmotic stress in H. discus discus. This study suggesting that FAA analysis is a useful tool to diagnose osmotic stress to H. discus discus.
Keywords
Free amino acid; Haliotis discus discus; Salinity stress; Osmotic stress;
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