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Effects of Diluents and Cryoprotectants on Sperm Cryopreservation in Starry Flounder (Platichthys stellatus)  

Lim, Han-Kyu (Aquaculture Management Team, National Fisheries Research and Development Institute)
An, Cheul-Min (Fisheries Resources Restoration Development and Management Center, National Fisheries Research and Development Institute)
Noh, Gyong-Ane (Department of Fisheries Biology, Graduate School, Pukyong National University)
Min, Byung-Hwa (Aquaculture Management Team, National Fisheries Research and Development Institute)
Publication Information
Journal of Aquaculture / v.20, no.3, 2007 , pp. 173-177 More about this Journal
Abstract
An experiment was performed to obtain cryopreservation techniques of starry flounder (Platichthys stellatus) sperm. Milt obtained from 24 males were cryopreserved using two diluents, artificial seminal plasma (ASP) and Stein's solution (SS) with three cryoprotectants, dimethyl sulfoxide (DMSO), methanol, and glycerol concentrated from 5% to 20%. Post-thaw sperm activity (motility and/or speed) revealed the highest in 10% DMSO and 15% methanol in ASP and SS as diluent. Motility and speed of cryopreserved sperm were decreased according to increase of glycerol concentration. To conclude, DMSO was a better cryoprotectant than methanol or glycerol for cryopreservation of starry flounder sperm. Glycerol was incongruent cryoprotectant because of toxic to starry flounder sperm. Most cryopreserved spermatozoa without cryoprotectant showed the enlarged head with granulated chromatin and ruptured plasma membrane by freezing and thawing injuries compared with unfrozen normal spermatozoa.
Keywords
Starry flounder; Platichthys stellatus; Cryopreservation; Cryoprotectant; Diluent;
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Times Cited By KSCI : 2  (Citation Analysis)
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