Browse > Article
http://dx.doi.org/10.3347/kjp.2020.58.2.181

Prevalence and Subtypes of Blastocystis in Alpacas, Vicugna pacos in Shanxi Province, China  

Ma, Ye-Ting (State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences)
Liu, Qing (College of Animal Science and Veterinary Medicine, Shanxi Agricultural University)
Xie, Shi-Chen (State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences)
Li, Xiao-Dong (State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences)
Ma, Yuan-Yuan (State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences)
Li, Tao-Shan (State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences)
Gao, Wen-Wei (College of Animal Science and Veterinary Medicine, Shanxi Agricultural University)
Zhu, Xing-Quan (State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences)
Publication Information
Parasites, Hosts and Diseases / v.58, no.2, 2020 , pp. 181-184 More about this Journal
Abstract
Blastocystis, an enteric protist, has been reported to be an important cause of protozoal gastrointestinal manifestations in humans and animals worldwide. Animals harboring certain Blastocystis subtypes (STs) may serve as a potential source of human infection. However, information about the prevalence and genetic diversity of Blastocystis in alpacas is limited. In the present study, a total of 366 fecal samples from alpacas in Shanxi Province, northern China, were examined for Blastocystis by PCR amplification of the small subunit rRNA gene, followed by sequencing and phylogenetic analysis. The prevalence of Blastocystis in alpacas was 23.8%, and gender difference in the prevalence of Blastocystis was observed. The most predominant Blastocystis ST was ST10, followed by ST14 and ST5. The detection of ST5, a potentially zoonotic genotype, indicates that alpacas harboring ST5 could be a potential source of human infection with Blastocystis. These data provide new insight into the prevalence and genetic diversity of Blastocystis in alpacas.
Keywords
Blastocystis; alpaca (Vicugna pacos); prevalence; subtype; Shanx;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Yakoob J, Jafri W, Beg MA, Abbas Z, Naz S, Islam M, Khan R. Irritable bowel syndrome: is it associated with genotypes of Blastocystis hominis. Parasitol Res 2010; 106: 1033-1038.   DOI
2 Casero RD, Mongi F, Sanchez A, Ramirez JD. Blastocystis and urticaria: examination of subtypes and morphotypes in an unusual clinical manifestation. Acta Trop 2015; 148: 156-161.   DOI
3 Poirier P, Wawrzyniak I, Vivares CP, Delbac F, El Alaoui H. New insights into Blastocystis spp.: a potential link with irritable bowel syndrome. PLoS Pathog 2012; 8: e1002545.   DOI
4 Deng L, Chai Y, Zhou Z, Liu H, Zhong Z, Hu Y, Fu H, Yue C, Peng G. Epidemiology of Blastocystis sp. infection in China: a systematic review. Parasite 2019; 26: 41.   DOI
5 Nagel R, Gray C, Bielefeldt-Ohmann H, Traub RJ. Features of Blastocystis spp. in xenic culture revealed by deconvolutional microscopy. Parasitol Res 2015; 114: 3237-3245.   DOI
6 Palasuwan A, Palasuwan D, Mahittikorn A, Chiabchalard R, Combes V, Popruk S. Subtype Distribution of Blastocystis in Communities along the Chao Phraya River, Thailand. Korean J Parasitol 2016; 54: 455-460.   DOI
7 Roberts T, Stark D, Harkness J, Ellis J. Subtype distribution of Blastocystis isolates from a variety of animals from New South Wales, Australia. Vet Parasitol 2013; 196: 85-89.   DOI
8 Li WC, Wang K, Gu Y. Occurrence of Blastocystis sp. and Pentatrichomonas hominis in sheep and goats in China. Parasit Vectors 2018; 11: 93.   DOI
9 Ramirez JD, Sanchez A, Hernandez C, Florez C, Bernal MC, Giraldo JC. Geographic distribution of human Blastocystis subtypes in South America. Infect Genet Evol 2016; 41: 32-35.   DOI
10 Maloney JG, Lombard JE, Urie NJ, Shivley CB, Santin M. Zoonotic and genetically diverse subtypes of Blastocystis in US pre-weaned dairy heifer calves. Parasitol Res 2019; 118: 575-582.   DOI
11 Zhang W, Ren G, Zhao W, Yang Z, Shen Y, Sun Y, Liu A, Cao J. Genotyping of Enterocytozoon bieneusi and subtyping of Blastocystis in cancer patients: relationship to diarrhea and assessment of zoonotic transmission. Front Microbiol 2017; 8: 1835.   DOI
12 Yan Y, Su S, Ye J, Lai X, Lai R, Liao H, Chen G, Zhang R, Hou Z, Luo X. Blastocystis sp. subtype 5: a possibly zoonotic genotype. Parasitol Res 2007; 101: 1527-1532.   DOI
13 Moosavi A, Haghighi A, Mojarad EN, Zayeri F, Alebouyeh M, Khazan H, Kazemi B, Zali MR. Genetic variability of Blastocystis sp. isolated from symptomatic and asymptomatic individuals in Iran. Parasitol Res 2012; 111: 2311-2315.   DOI
14 Kadwell M, Fernandez M, Stanley HF, Baldi R, Wheeler JC, Rosadio R, Bruford MW. Genetic analysis reveals the wild ancestors of the llama and the alpaca. Proc Biol Sci 2001; 268: 2575-2584.   DOI
15 Wang W, Owen H, Traub RJ, Cuttell L, Inpankaew T, Bielefeldt-Ohmann H. Molecular epidemiology of Blastocystis in pigs and their in-contact humans in Southeast Queensland, Australia, and Cambodia. Vet Parasitol 2014; 203: 264-269.   DOI
16 Forsell J, Granlund M, Stensvold CR, Clark CG, Evengard B. Subtype analysis of Blastocystis isolates in Swedish patients. Eur J Clin Microbiol Infect Dis 2012; 31: 1689-1696.   DOI
17 Alfellani MA, Taner-Mulla D, Jacob AS, Imeede CA, Yoshikawa H, Stensvold CR, Clark CG. Genetic diversity of Blastocystis in livestock and zoo animals. Protist 2013; 164: 497-509.   DOI
18 Zhao GH, Hu XF, Liu TL, Hu RS, Yu ZQ, Yang WB, Wu YL, Yu SK, Song JK. Molecular characterization of Blastocystis sp. in captive wild animals in Qinling Mountains. Parasitol Res 2017; 116: 2327-2333.   DOI
19 Stensvold CR, Alfellani MA, Norskov-Lauritsen S, Prip K, Victory EL, Maddox C, Nielsen HV, Clark CG. Subtype distribution of Blastocystis isolates from synanthropic and zoo animals and identification of a new subtype. Int J Parasitol 2009; 39: 473-479.   DOI
20 Zhu W, Tao W, Gong B, Yang H, Li Y, Song M, Lu Y, Li W. First report of Blastocystis infections in cattle in China. Vet Parasitol 2017; 246: 38-42.   DOI
21 Stenzel DJ, Cassidy MF, Boreham PF. Morphology of Blastocystis sp. isolated from circus animals. Int J Parasitol 1993; 23: 685-687.   DOI
22 Boreham PF, Stenzel DJ. Blastocystis in humans and animals: morphology, biology, and epizootiology. Adv Parasitol 1993; 32: 1-70.   DOI
23 Amin OM. Seasonal prevalence of intestinal parasites in the United States during 2000. Am J Trop Med Hyg 2002; 66: 799-803.   DOI
24 Abe N. Molecular and phylogenetic analysis of Blastocystis isolates from various hosts. Vet Parasitol 2004; 120: 235-242.   DOI
25 Parkar U, Traub RJ, Vitali S, Elliot A, Levecke B, Robertson I, Geurden T, Steele J, Drake B, Thompson RC. Molecular characterization of Blastocystis isolates from zoo animals and their animal-keepers. Vet Parasitol 2010; 169: 8-17.   DOI